| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,214 | T→C | 57.7% | F276F (TTT→TTC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,214 | 0 | T | C | 57.7% | 55.8 / 124.8 | 97 | F276F (TTT→TTC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (12/29); new base C (19/37); total (31/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAA > W3110S.gb/71146‑71284 | ggTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTc < 1:2214867/70‑1 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGcc > 1:1735555/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGcc > 1:5568315/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc > 1:910648/1‑71 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:3284552/1‑70 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:2801030/1‑70 (MQ=255) cAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgtg > 1:4787991/1‑70 (MQ=255) cAACTCTATTTCTAGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:324965/1‑70 (MQ=255) tctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga > 1:2724489/1‑70 (MQ=255) ctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaagaa > 1:2999250/1‑70 (MQ=255) ctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaagaa > 1:225872/1‑70 (MQ=255) ctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaagaa > 1:409068/1‑70 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:278649/1‑70 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaa < 1:2357573/69‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:2774374/68‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaa < 1:1971557/69‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaa < 1:23106/69‑1 (MQ=255) tctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt > 1:4189318/1‑70 (MQ=255) tctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt > 1:2129432/1‑70 (MQ=255) tctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt > 1:84802/1‑70 (MQ=255) ctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa < 1:990963/64‑1 (MQ=255) ctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGGGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTg < 1:522545/70‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGACCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGaa > 1:5536501/1‑69 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:4550850/71‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:4084637/70‑1 (MQ=255) cgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:4299688/71‑1 (MQ=255) cgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:5332735/71‑1 (MQ=255) cgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:3733854/71‑1 (MQ=255) gAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:1242951/71‑1 (MQ=255) gAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:637450/71‑1 (MQ=255) ggTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgatg < 1:5423305/70‑1 (MQ=255) ggTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgatg < 1:2072828/70‑1 (MQ=255) aGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgatg < 1:5116838/71‑1 (MQ=255) gTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaaga < 1:4819851/56‑1 (MQ=255) tATTT‑AAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:762047/67‑1 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg < 1:4594700/49‑1 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAg < 1:3873461/70‑1 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAg < 1:4374931/70‑1 (MQ=255) ttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGcc > 1:3513432/1‑71 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg > 1:5217672/1‑56 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCg > 1:1877747/1‑71 (MQ=255) aaaaaTGCAGCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc < 1:2628111/71‑1 (MQ=255) aaaTGCACCGGGGCCAGCCGGAGCGAGTTTCGTGCCGGTTGTgaaga < 1:4105228/47‑1 (MQ=255) aaaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc < 1:3401954/36‑1 (MQ=255) aaTGCACCGGGGCCAGCCCTAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg < 1:3978890/71‑1 (MQ=255) aaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg < 1:3741809/71‑1 (MQ=255) aaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg < 1:2102641/71‑1 (MQ=255) aaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg < 1:4850485/71‑1 (MQ=255) aaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg < 1:5136143/71‑1 (MQ=255) tGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt < 1:4210080/71‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:4654814/71‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:4838708/71‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:221994/71‑1 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa < 1:85732/71‑1 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa < 1:4709822/71‑1 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa < 1:1248634‑M1/71‑2 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa < 1:1255106‑M1/71‑2 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa < 1:4616943‑M1/71‑2 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa < 1:1535211‑M1/71‑2 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa < 1:472325‑M1/71‑2 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa < 1:5153104‑M1/71‑2 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa < 1:5052301‑M1/71‑2 (MQ=255) gCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa < 1:1645806‑M1/70‑2 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:783526/44‑1 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:1021945/44‑1 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:5338850/44‑1 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:97348/44‑1 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:1015348/44‑1 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:2826477/44‑1 (MQ=255) cccGAGCGAGTTTCGTGCCGGTCGTGAAGAAAAAGTGAatgat < 1:4644286/43‑1 (MQ=255) cccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa > 1:725552/1‑63 (MQ=255) cccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa > 1:916681‑M1/1‑64 (MQ=255) cccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa > 1:2108528‑M1/1‑64 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:2293474/1‑70 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:5300293/1‑70 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:3107108/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:344277/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:750343/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:2268500/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:5038525/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:5293736/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:3665404/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:1906232/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:4714809/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:1751391/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:3113495/52‑1 (MQ=255) agcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGa > 1:3949424/1‑70 (MQ=255) agcgagTTCCGTGCGGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTGATaa < 1:105474/60‑1 (MQ=255) agcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCa < 1:956542/49‑1 (MQ=255) agcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGt > 1:21277/1‑52 (MQ=255) agcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGt > 1:7675/1‑52 (MQ=255) agcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGt > 1:4439055/1‑52 (MQ=255) gcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg < 1:1140511/50‑1 (MQ=255) cgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg > 1:4655150/1‑49 (MQ=255) cgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg > 1:4911165/1‑49 (MQ=255) cgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccctaagt > 1:473544‑M1/1‑58 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa > 1:5252242/1‑52 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa > 1:4939962/1‑52 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa > 1:4933526/1‑52 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACaa > 1:4302729/1‑71 (MQ=255) | GGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAA > W3110S.gb/71146‑71284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |