Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2176983–2178179 2178181 3–1199 40 [0] [0] 39 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 21769820 (0.000)29 (1.430) 16/88 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?W3110S.gb 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

TCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176910‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  W3110S.gb/2178182‑2178250
                                                                                                                                              
TCGTTATAATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTT                                                                         <  1:528861/71‑1
  GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       >  1:2219292/1‑71
     ATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:2068020/1‑71
     ATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:2169805/1‑71
     ATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:128635/1‑71
     ATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:36176/1‑71
              CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                           <  1:1147585/71‑1
              CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCC                                                            <  1:338896/70‑1
                   GCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:376680/69‑1
                         AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:1818937/63‑1
                           TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:1573687/68‑1
                              ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                           <  1:43184/71‑1
                              ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                           <  1:1188803/71‑1
                                TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTG                                            <  1:539550/68‑1
                                TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTT                                                                         <  1:1583537/39‑1
                                 TTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATG                                                                   <  1:200639/44‑1
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:1491620/1‑69
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                      >  1:2025512/1‑56
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:1046825/1‑46
                                   ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                      >  1:405484/1‑71
                                   ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:813328/60‑1
                                   ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:1034169/60‑1
                                    TTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAAT                                        >  1:601099/1‑68
                                           GCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                           <  1:1334798/58‑1
                                           GCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                           <  1:1145444/58‑1
                                           GCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAAT                                                     <  1:1582717/48‑1
                                           GCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAAT                                                     <  1:333490/48‑1
                                            CCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCT                             >  1:683397/1‑71
                                             CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:1288218/1‑58
                                              TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:628817/49‑1
                                              TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAAT                                                     <  1:404957/45‑1
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                      >  1:1020872/1‑42
                                                   AGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:1159299/37‑1
                                                      TAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTAT                   >  1:1192635/1‑71
                                                           GGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG              >  1:1589811/1‑71
                                                           GGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                  >  1:2145619/1‑51
                                                            GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGT             <  1:1311070/71‑1
                                                                   ACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAAT      <  1:551325/71‑1
                                                                     TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTC    >  1:1973336/1‑71
                                                                       AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:519169/1‑71
                                                                       AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:1044757/1‑71
                                                                        GCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCC                <  1:399202/56‑1
                                                                                                                                              
TCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176910‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  W3110S.gb/2178182‑2178250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.