Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6310CT100.0% 77.1 / NA 27Q246Y (CAG→TAT) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTA  >  W3110S.gb/1303568‑1303685
                                                               |                                                      
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                  <  1:1708736/70‑1 (MQ=255)
  cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                >  1:2067218/1‑70 (MQ=255)
        aaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGc                                         >  1:473948/1‑71 (MQ=255)
         aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                        <  1:2098005/71‑1 (MQ=255)
          tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                        >  1:143570/1‑70 (MQ=255)
          tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                        >  1:1722612/1‑70 (MQ=255)
                 tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                            >  1:1554987/1‑59 (MQ=255)
                    aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                             <  1:1048589/71‑1 (MQ=255)
                          cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                            <  1:763877/66‑1 (MQ=255)
                          cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                       <  1:2137050/71‑1 (MQ=255)
                                 tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                              <  1:1861222/41‑1 (MQ=38)
                                 tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                             <  1:1003242/42‑1 (MQ=38)
                                  aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                            >  1:1485548/1‑42 (MQ=38)
                                  aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                      >  1:795818/1‑48 (MQ=39)
                                  aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                      >  1:1436491/1‑48 (MQ=39)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc               <  1:1947275/70‑1 (MQ=255)
                                     ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                            <  1:1086203/55‑1 (MQ=255)
                                      gAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                            >  1:879724/1‑38 (MQ=37)
                                      gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAcc           >  1:844554/1‑71 (MQ=255)
                                         caacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                      <  1:1281869/57‑1 (MQ=255)
                                           acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAc      >  1:1706278/1‑71 (MQ=255)
                                             gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt                        <  1:1955758/51‑1 (MQ=255)
                                             gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                   >  1:1342637/1‑56 (MQ=255)
                                             gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                   >  1:945593/1‑56 (MQ=255)
                                               gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTa  <  1:759215/71‑1 (MQ=255)
                                                    ccGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa             >  1:767210/1‑55 (MQ=255)
                                                           taACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                     <  1:846836/40‑1 (MQ=38)
                                                               |                                                      
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTA  >  W3110S.gb/1303568‑1303685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: