| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,303,633 | G→T | 100% | Q246Y (CAG→TAT) | oppA → | oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,303,633 | 0 | G | T | 100.0% | 77.2 / NA | 27 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/13); total (14/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTA > W3110S.gb/1303568‑1303685 | aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa < 1:1708736/70‑1 (MQ=255) cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc > 1:2067218/1‑70 (MQ=255) aaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGc > 1:473948/1‑71 (MQ=255) aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:2098005/71‑1 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc > 1:143570/1‑70 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc > 1:1722612/1‑70 (MQ=255) tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt > 1:1554987/1‑59 (MQ=255) aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:1048589/71‑1 (MQ=255) cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:763877/66‑1 (MQ=255) cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa < 1:2137050/71‑1 (MQ=255) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:1861222/41‑1 (MQ=38) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt < 1:1003242/42‑1 (MQ=38) aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt > 1:1485548/1‑42 (MQ=38) aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa > 1:795818/1‑48 (MQ=39) aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa > 1:1436491/1‑48 (MQ=39) cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc < 1:1947275/70‑1 (MQ=255) ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:1086203/55‑1 (MQ=255) gAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt > 1:879724/1‑38 (MQ=37) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAcc > 1:844554/1‑71 (MQ=255) caacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc < 1:1281869/57‑1 (MQ=255) acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAc > 1:1706278/1‑71 (MQ=255) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt < 1:1955758/51‑1 (MQ=255) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa > 1:1342637/1‑56 (MQ=255) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa > 1:945593/1‑56 (MQ=255) gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTa < 1:759215/71‑1 (MQ=255) ccGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa > 1:767210/1‑55 (MQ=255) taACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:846836/40‑1 (MQ=38) | AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTA > W3110S.gb/1303568‑1303685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |