| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,638,314 | G→C | 12.8% | intergenic (‑233/‑156) | nohA ← / → ynfO | predicted packaging protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,638,314 | 0 | G | C | 12.8% | 100.0 / 6.0 | 39 | intergenic (‑233/‑156) | nohA/ynfO | predicted packaging protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/21); new base C (2/3); total (15/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
CGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAATTATGACGCAGG > W3110S.gb/1638247‑1638384 | cGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAtt < 1:1940145/71‑1 (MQ=255) ccccGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgt < 1:2079575/71‑1 (MQ=255) gTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAtt < 1:1182772/46‑1 (MQ=255) gTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTa > 1:1560683/1‑54 (MQ=255) tGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTatc < 1:790595/43‑1 (MQ=255) tGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTatc < 1:1075783/43‑1 (MQ=255) tGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGc > 1:2185634/1‑71 (MQ=255) ggATCACCGGAAAGGACCCGTAAAGTGATAATCATTatc < 1:115147/39‑1 (MQ=255) ggATCACCGGAAAGGACCCGTAAAGTGATAATCATTatc < 1:400487/39‑1 (MQ=255) ggATCACCGGAAAGGACCCGTAAAGTGATAATCATTatc < 1:294776/39‑1 (MQ=255) gATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgtg < 1:64004/61‑1 (MQ=255) gATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgtg < 1:1746997/61‑1 (MQ=255) aTCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCaa < 1:935740/71‑1 (MQ=255) aTCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCaa < 1:1043080/71‑1 (MQ=255) aTCACCGGAAAGGACCCGTAAAGTGATAATCATTatcac > 1:1480717/1‑38 (MQ=21) aTCACCGGAAAGGACCCGTAAAGTGATAATCATTatcac > 1:2168963/1‑38 (MQ=21) aGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtg < 1:322955/46‑1 (MQ=255) aCCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAaata < 1:357363/71‑1 (MQ=255) ccGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAaataat > 1:2217893/1‑71 (MQ=255) gTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGa > 1:1090500/1‑45 (MQ=255) gTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATc > 1:352683/1‑70 (MQ=255) tAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgtg < 1:1442690/42‑1 (MQ=255) tAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAaataa > 1:550147/1‑67 (MQ=255) tAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATc < 1:1023078/69‑1 (MQ=255) tAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATc < 1:521531/69‑1 (MQ=255) aaGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCTAATAATCAAt > 1:1925870/1‑70 (MQ=255) aaGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCaa > 1:843472/1‑69 (MQ=255) aaGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAAt < 1:314334/70‑1 (MQ=255) aaGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAAt > 1:1531243/1‑70 (MQ=255) aaGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAAt > 1:1513725/1‑70 (MQ=255) aaGTGAAAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAAt > 1:1284/1‑70 (MQ=255) gTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAATTAt < 1:189866/71‑1 (MQ=255) aTAATGATTATCATCTACATATCACAACGTGCGTGGa < 1:1524722/37‑1 (MQ=255) aTAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATc < 1:1450558/62‑1 (MQ=255) tAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAATTATGAc > 1:1915941/1‑70 (MQ=255) aaTGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAATTATGACg > 1:144566/1‑70 (MQ=255) tGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCaa < 1:481728/42‑1 (MQ=255) tGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCaa < 1:521715/42‑1 (MQ=255) tGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCaa < 1:553816/42‑1 (MQ=255) gATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAATTATGACGCAgg > 1:1663349/1‑71 (MQ=255) | CGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAATTATGACGCAGG > W3110S.gb/1638247‑1638384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |