Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,326 T→C 100% pseudogene (459/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3260TC100.0% 78.6 / NA 24pseudogene (459/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCTGCGTGGCGCTGGTAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATC  >  W3110S.gb/3824265‑3824390
                                                             |                                                                
ccTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGa                                                           >  1:1669843/1‑69 (MQ=255)
    cGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAggg                                                      <  1:304977/70‑1 (MQ=255)
        gcgcTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATgg                                                  >  1:1257381/1‑70 (MQ=255)
          gcTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAg                                               <  1:211996/71‑1 (MQ=255)
            tGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGcc                                             >  1:942051/1‑71 (MQ=255)
               caGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATg                                                   >  1:990270/2‑62 (MQ=255)
                   tGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGaaa                                                         >  1:1805877/1‑52 (MQ=255)
                         aCAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATg                                                            >  1:1824603/1‑43 (MQ=255)
                         aCAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAggg                                                      >  1:267461/1‑49 (MQ=255)
                          cAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGc                                              >  1:2196579/1‑56 (MQ=255)
                          cAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGt                                  >  1:793614/1‑68 (MQ=255)
                            gAAGCTGGTGGAAAACGGCAAGCTGAATACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCggta                             <  1:199279/71‑3 (MQ=255)
                            gAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGc                                           <  1:1812442/57‑1 (MQ=255)
                              aGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAgg                                                       <  1:1264752/43‑1 (MQ=255)
                                       aaaaCGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggt                  <  1:1499352/71‑2 (MQ=255)
                                           cGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAg              >  1:1081814/1‑71 (MQ=255)
                                              cAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa           <  1:2038869/71‑3 (MQ=255)
                                                 gCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTg                                   <  1:1053373/44‑1 (MQ=255)
                                                 gCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTg                                   <  1:2139580/44‑1 (MQ=255)
                                                  cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTaaa                      >  1:1405512/1‑56 (MQ=255)
                                                  cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg       <  1:616804/71‑1 (MQ=255)
                                                   tGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg       <  1:961848/70‑1 (MQ=255)
                                                   tGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg       <  1:1046261/70‑1 (MQ=255)
                                                     aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACgg                   >  1:1190845/1‑56 (MQ=255)
                                                       aaccaaccCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATc  <  1:1924906/71‑1 (MQ=255)
                                                             |                                                                
CCTGCGTGGCGCTGGTAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATC  >  W3110S.gb/3824265‑3824390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: