| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,214 | T→C | 52.3% | F276F (TTT→TTC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,214 | 0 | T | C | 52.3% | 4.9 / 61.8 | 44 | F276F (TTT→TTC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (8/13); new base C (14/9); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
GACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACA > W3110S.gb/71152‑71283 | gACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCg < 1:1894593/70‑1 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:2045439/1‑70 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:2012485/1‑70 (MQ=255) ctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa > 1:167348/1‑70 (MQ=255) tATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa > 1:638666/1‑70 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaa < 1:1859851/69‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg > 1:2089516/1‑71 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAg > 1:787833/1‑71 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAg > 1:416690/1‑71 (MQ=255) tctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt > 1:1395384/1‑70 (MQ=255) tcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa < 1:821612/63‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:1428615/70‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:1113662/71‑1 (MQ=255) cgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGaa < 1:247495/68‑1 (MQ=255) cgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:691794/71‑1 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt < 1:1197724/59‑1 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCg > 1:995211/1‑71 (MQ=255) aaaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGt > 1:1625566/1‑71 (MQ=255) aaaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGt > 1:978200/1‑71 (MQ=255) aaaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGt > 1:975652/1‑71 (MQ=255) aaaaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc < 1:1036340/70‑1 (MQ=255) aaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCa < 1:22270/71‑1 (MQ=255) aTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt > 1:1472485/1‑38 (MQ=255) cACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa > 1:440616/1‑46 (MQ=255) cGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:1664750/49‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgat > 1:722400/1‑51 (MQ=255) gggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa < 1:1210756/39‑1 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg > 1:82324/1‑44 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:1244434/49‑1 (MQ=255) ccAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTg < 1:1652905/41‑1 (MQ=255) ccAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTg < 1:181349/41‑1 (MQ=255) ccAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCAt < 1:275695/66‑1 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGt > 1:1439918/1‑70 (MQ=255) gCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:1210284/64‑1 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatga > 1:1973492/1‑41 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:714527/1‑70 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACg < 1:302803/71‑1 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACg < 1:931039/71‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:1705134/41‑1 (MQ=255) agcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:1589859/37‑1 (MQ=255) agcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGaa < 1:1793914/71‑1 (MQ=255) agcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccct > 1:1268716‑M1/1‑60 (MQ=255) gcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAAt > 1:157624/1‑71 (MQ=255) agTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa > 1:873477/1‑56 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACa > 1:1232899/1‑70 (MQ=255) | GACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACA > W3110S.gb/71152‑71283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |