| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,892,019:1 | +GC | 100% | intergenic (‑437/+54) | gudP ← / ← ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,892,019 | 1 | . | G | 100.0% | 209.3 / NA | 60 | intergenic (‑437/+54) | gudP/ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (33/27); total (33/27) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 1,892,019 | 2 | . | C | 100.0% | 208.5 / NA | 60 | intergenic (‑437/+54) | gudP/ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (33/27); total (33/27) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAA > minE/1891951‑1892076 || cTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCag < 1:789688/70‑2 (MQ=255) gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct < 1:1225496/69‑4 (MQ=255) ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAg > 1:414005/1‑70 (MQ=255) tGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc > 1:2487044/1‑71 (MQ=255) tGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc > 1:2364049/1‑71 (MQ=255) gAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc > 1:913381/1‑71 (MQ=255) gAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc > 1:3827292/1‑71 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc < 1:3916246/70‑1 (MQ=255) aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg > 1:335679/1‑71 (MQ=255) aGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGt < 1:285135/71‑1 (MQ=255) gattcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc > 1:1044333/1‑71 (MQ=255) gattcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc > 1:122424/1‑71 (MQ=255) gattcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc > 1:2337461/1‑71 (MQ=255) ttcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCgg < 1:1180183/71‑1 (MQ=255) ttcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCgg < 1:3999776/71‑1 (MQ=255) ttcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg > 1:42657/1‑70 (MQ=255) tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa > 1:220326/1‑71 (MQ=255) gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt < 1:2595183/70‑1 (MQ=255) gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt > 1:1632627/1‑70 (MQ=255) gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa < 1:1289629/71‑1 (MQ=255) gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa < 1:4113041/71‑1 (MQ=255) attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca > 1:3111225/1‑55 (MQ=255) attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca > 1:1596669/1‑55 (MQ=255) attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa < 1:64744/71‑1 (MQ=255) ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt > 1:308400/1‑68 (MQ=255) ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATAAGCTAAGCACAGAACCGGGTCGGATaaa > 1:3507146/1‑70 (MQ=255) tcAGCACCTCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa > 1:836157/1‑71 (MQ=255) tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg > 1:2323771/1‑70 (MQ=255) cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa < 1:3947748/70‑1 (MQ=255) cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc > 1:907608/1‑71 (MQ=255) cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc > 1:1707263/1‑71 (MQ=255) cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt > 1:640098/1‑71 (MQ=255) cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc < 1:1336674/71‑1 (MQ=255) gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa > 1:2442540/1‑61 (MQ=255) cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc < 1:460337/54‑1 (MQ=255) cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg < 1:2883854/55‑1 (MQ=255) tAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt < 1:2452528/57‑1 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct > 1:2008661/1‑71 (MQ=255) aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAg < 1:4094262/42‑1 (MQ=255) aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAg < 1:150945/42‑1 (MQ=255) aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc < 1:4092920/71‑1 (MQ=255) aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct > 1:1143293/1‑70 (MQ=255) aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg > 1:486456/1‑46 (MQ=255) aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt < 1:1200719/53‑1 (MQ=255) aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt < 1:487517/53‑1 (MQ=255) aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc > 1:2580096/1‑64 (MQ=255) aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAt < 1:1076995/71‑1 (MQ=255) aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAt < 1:1657670/71‑1 (MQ=255) cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc > 1:1708637/1‑71 (MQ=255) cATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCggg < 1:1326638/43‑1 (MQ=255) aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg < 1:1061905/69‑1 (MQ=255) aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGAc < 1:745883/71‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGGTa > 1:3461465/1‑48 (MQ=255) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGCTa > 1:2983141/1‑46 (MQ=255) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt < 1:1835633/70‑1 (MQ=255) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACAGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt > 1:833959/1‑70 (MQ=255) cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa > 1:3084193/1‑69 (MQ=255) gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTg < 1:2490622/71‑1 (MQ=39) tcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGtt > 1:2917436/1‑71 (MQ=255) gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt < 1:3200323/70‑1 (MQ=38) gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGtt < 1:1473672/69‑1 (MQ=255) ctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTaa > 1:2653013/1‑71 (MQ=255) || CTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAA > minE/1891951‑1892076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |