| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 573,576 | A→G | 20.7% | G135G (GGA→GGG) | ybjD → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 573,576 | 0 | A | G | 20.7% | 226.8 / 57.7 | 106 | G135G (GGA→GGG) | ybjD | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base A (38/46); new base G (5/17); total (43/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
CTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGG > minE/573511‑573637 | cTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATcc > 1:3662240/1‑71 (MQ=255) gAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGGCATcc < 1:1811338/68‑1 (MQ=255) gAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGAt > 1:2407953/1‑71 (MQ=255) aGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTg < 1:3484633/71‑1 (MQ=255) agagTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTc > 1:2877887/1‑71 (MQ=255) agagTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTc > 1:1352184/1‑71 (MQ=255) gagTGCGGAAGACGGCAGGGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGCTTGATGTCg < 1:221604/71‑1 (MQ=255) gagTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCg < 1:1346331/71‑1 (MQ=255) gagTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCg < 1:63869/71‑1 (MQ=255) gagTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCg < 1:2338866/71‑1 (MQ=255) gagTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCg < 1:3849279/71‑1 (MQ=255) agTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGCCGa < 1:3734750/71‑1 (MQ=255) gTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAg < 1:722185/71‑1 (MQ=255) tGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGAt < 1:2149560/60‑1 (MQ=255) tGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGAt < 1:2386634/60‑1 (MQ=255) cGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGa < 1:3715114/70‑1 (MQ=255) aaGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTCGAGGatat > 1:2104044/1‑70 (MQ=255) aaGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGatat > 1:378705/1‑70 (MQ=255) aaGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGatat > 1:3400898/1‑70 (MQ=255) aGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTa < 1:1734768/71‑1 (MQ=255) aGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTa < 1:840138/71‑1 (MQ=255) aCGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAAc < 1:1347896/71‑1 (MQ=255) cGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTCGAGGATATTAACg < 1:2883912/71‑1 (MQ=255) cGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTCGAGGATATTAACg < 1:2776619/71‑1 (MQ=255) cGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAAc > 1:2637270/1‑70 (MQ=255) ggCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGa > 1:3398891/1‑71 (MQ=255) gCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGAt > 1:210652/1‑71 (MQ=255) gCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGAt > 1:3223848/1‑71 (MQ=255) gCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGAt < 1:1364521/71‑1 (MQ=255) gCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGAt < 1:2527020/71‑1 (MQ=255) aGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTCGAGGat < 1:1463232/60‑1 (MQ=255) aGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTCGAGGat < 1:490906/60‑1 (MQ=255) aGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCa < 1:2769241/71‑1 (MQ=255) aGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCa < 1:3304066/71‑1 (MQ=255) gCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTCGAGGat < 1:2971255/59‑1 (MQ=255) gCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAg < 1:3108405/71‑1 (MQ=255) gCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAg > 1:2933774/1‑71 (MQ=255) cGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACAT‑CAATTGATGTCGAGGATATTaa < 1:3912786/62‑1 (MQ=255) gTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAgg > 1:1112228/1‑70 (MQ=255) tgatgaCACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGAtt < 1:1211445/44‑1 (MQ=255) tgatgaCACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGAtt < 1:1881657/44‑1 (MQ=255) tgatgaCACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGAtt < 1:4121505/44‑1 (MQ=255) tgatgaCACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGAtt < 1:487206/44‑1 (MQ=255) gatgaCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGa > 1:3472624/1‑54 (MQ=255) gatgaCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCAc > 1:1867063/1‑71 (MQ=255) atgaCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGa > 1:937613/1‑40 (MQ=255) atgaAACTGCGCAGTTTTCTCGATAAAGACGGACATCCGa > 1:1052164/1‑40 (MQ=255) tgaCACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGAtt < 1:3100915/41‑1 (MQ=255) tgaCACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGAtt < 1:3065666/41‑1 (MQ=255) tgaCACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGAtt < 1:2971298/41‑1 (MQ=255) tgaCACTGCGCAGTTTTCTCGATAAAGACGGGCATCCGAtt < 1:543631/41‑1 (MQ=255) tgaCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGc > 1:2018564/1‑71 (MQ=255) acacTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGcc < 1:1496171/70‑1 (MQ=255) cacTGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCAt < 1:521971/71‑1 (MQ=255) cacTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGata > 1:2905704/1‑51 (MQ=255) cacTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGata > 1:885867/1‑51 (MQ=255) cacTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGata > 1:3504220/1‑51 (MQ=255) cacTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCAt > 1:1082237/1‑71 (MQ=255) acTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGAtct > 1:586721/1‑40 (MQ=255) tGCGCAGTTTTCTCGATAAAGACGGGCATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATc < 1:3620141/69‑1 (MQ=255) tGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTg > 1:3814083/1‑71 (MQ=255) gcgcAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTgg > 1:1602490/1‑71 (MQ=255) gcgcAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTgg > 1:2870535/1‑71 (MQ=255) cgcAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATc > 1:1125548/1‑55 (MQ=255) cgcAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTg < 1:2673406/69‑1 (MQ=255) cAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGc < 1:3158800/71‑1 (MQ=255) ttttCTCGATAAAGACGGACATCCGATTGATGTCGAGGa < 1:1836661/39‑1 (MQ=255) ttttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAgg < 1:880918/53‑1 (MQ=255) ttttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGc < 1:75646/54‑1 (MQ=255) ttttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGc < 1:3173645/54‑1 (MQ=255) ttttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGtt > 1:3207297/1‑71 (MQ=255) tttCTCGATAAAGACGGGCATCCGATTGATGTCGAGGATATTAACGAt > 1:1877092/1‑48 (MQ=255) tttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGtt > 1:785292/1‑70 (MQ=255) tttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGtt > 1:123023/1‑70 (MQ=255) tttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGtt > 1:1307848/1‑70 (MQ=255) ttCTCGATAAAGACGGGCATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGttt < 1:3296513/70‑1 (MQ=255) ttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATc < 1:745234/60‑1 (MQ=255) ttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATc < 1:711930/60‑1 (MQ=255) ttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGttt < 1:2189917/70‑1 (MQ=255) ttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGttt > 1:10607/1‑70 (MQ=255) ctcGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGc < 1:2162895/50‑1 (MQ=255) ctcGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGc < 1:1356063/50‑1 (MQ=255) tcGATAAAGACGGACATCCGATTGATGTCGAGGATAtt < 1:2394780/38‑1 (MQ=255) tcGATAAAGACGGACATCCGATTGATGTCGAGGATAtt < 1:2437491/38‑1 (MQ=255) tcGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGttt < 1:222621/67‑1 (MQ=255) tcGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAAt > 1:2291869/1‑70 (MQ=255) tcGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAAt > 1:1081895/1‑70 (MQ=255) tcGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATg > 1:2921238/1‑71 (MQ=255) gATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGcc < 1:3879224/71‑1 (MQ=255) aTAAAGACGGGCATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCg > 1:1728188/1‑71 (MQ=255) aTAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAgg < 1:1752018/45‑1 (MQ=255) aTAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAgg < 1:3584624/45‑1 (MQ=255) aTAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTg < 1:594980/56‑1 (MQ=255) tAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCgg < 1:1029845/71‑1 (MQ=255) tAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCgg < 1:3228505/71‑1 (MQ=255) aaaGACGGACATCCGATTGATGTCGAGGATATTAAGGATCAGGCACGcc > 1:3249167/1‑49 (MQ=255) aaaGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGcc > 1:2122945/1‑49 (MQ=255) aaaGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGt > 1:3677792/1‑56 (MQ=255) aGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTgg > 1:3067642/1‑53 (MQ=255) cGGACATCCGATTGATGTCGAGGATATTAAGGATCAGGCACGCCATCTGGTg > 1:2128629/1‑52 (MQ=255) | CTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGG > minE/573511‑573637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |