| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 826,990 | G→A | 14.9% | N173N (AAC→AAT) | narL ← | DNA‑binding response regulator in two‑component regulatory system with NarX (or NarQ) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 826,990 | 0 | G | A | 14.9% | 178.7 / 20.8 | 67 | N173N (AAC→AAT) | narL | DNA‑binding response regulator in two‑component regulatory system with NarX (or NarQ) |
| Reads supporting (aligned to +/- strand): ref base G (38/19); new base A (3/7); total (41/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.85e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCG > minE/826920‑827058 | tcttcAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTg > 1:3958990/1‑71 (MQ=255) cttcAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTg < 1:177533/70‑1 (MQ=255) cttcAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTg < 1:1445105/70‑1 (MQ=255) cAGCATGTGCTTTACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGt < 1:111606/68‑1 (MQ=255) cAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGt < 1:1147690/68‑1 (MQ=255) cAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGt < 1:414506/68‑1 (MQ=255) cAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCg > 1:3375510/1‑71 (MQ=255) aGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCgg > 1:1021884/1‑71 (MQ=255) tgCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAAcc > 1:146018/1‑71 (MQ=255) cGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCa < 1:2031980/71‑1 (MQ=255) cACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAAt > 1:2545881/1‑69 (MQ=255) aCTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAg > 1:1215267/1‑71 (MQ=255) cTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCaa < 1:2462129/66‑1 (MQ=255) ttACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCt > 1:2992064/1‑57 (MQ=255) cTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTgag < 1:3644285/70‑1 (MQ=255) tgtgCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAAccc < 1:2149272/52‑1 (MQ=255) tgtgCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAAccc < 1:3029747/52‑1 (MQ=255) gtgCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGc > 1:1809819/1‑45 (MQ=255) tgCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAata > 1:3982976/1‑71 (MQ=255) cTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATc > 1:2925188/1‑71 (MQ=255) tCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATcgc < 1:3040549/70‑1 (MQ=255) tCGGTGATATCCAGGCGGCGGGCAATCATCTTATTCGGCAAACCCTGGGCAATCAGCTTGATAATATcgcg > 1:139631/1‑71 (MQ=255) cGGTGATATCCAGGCGGCGGGCAATCATCTTATTCGGCAAACCCTGGGCAATCAGCTTgag > 1:318033/1‑61 (MQ=255) cGGTGATATCCAGGCGGCGGGCAATCATCTTATTCGGCAAACCCTGGGCAATCAGCTTGAGAATATcgcg < 1:2128160/70‑1 (MQ=255) ggTGATATCCAGGCGGCGGGCAATCATCTTGTTCgg > 1:3292379/1‑36 (MQ=255) ggTGATATCCAGGCGGCGGGCAATCATCTTATTCGGCAAACCCTGCGCAATCAGCTTGAGAATATCgcgc < 1:3425557/70‑1 (MQ=255) gTGATATCCAGGCGGCGGGCAATCATCTTATTCGGCAAACCCTGGGCAATCAGc < 1:1777104/54‑1 (MQ=255) tatCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGaa > 1:818911/1‑57 (MQ=255) tatCCAGGCGGCGGGCAATCATCTTGTTAGGCAAACCCTGGGCAATCAGCTTGAGaa > 1:172436/1‑57 (MQ=255) tCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGaa < 1:4113244/55‑1 (MQ=255) tCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTgg < 1:47960/71‑1 (MQ=255) cAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTg > 1:1038910/1‑36 (MQ=255) cAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAata > 1:1283258/1‑55 (MQ=255) cAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTgggg > 1:4059526/1‑71 (MQ=255) cAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTgggg > 1:2553406/1‑71 (MQ=255) gcggcgGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGaa > 1:2409763/1‑50 (MQ=255) gcggcgGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGtt > 1:3946863/1‑70 (MQ=255) gcggcgGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTa < 1:1919354/71‑1 (MQ=255) gcggcgGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTa < 1:2890090/71‑1 (MQ=255) gcggcgGGCAATCATCTTATTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTa < 1:2134456/71‑1 (MQ=255) cggcggGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTaa > 1:2452850/1‑71 (MQ=255) gcggGCAATCATCTTATTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTgggg < 1:2428141/65‑1 (MQ=255) gcggGCAATCATCTTATTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTgggg < 1:2764155/65‑1 (MQ=255) gggCAATCATCTTATTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTaa > 1:1497627/1‑67 (MQ=255) ggCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGt > 1:2982234/1‑71 (MQ=255) ggCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGt < 1:2144187/71‑1 (MQ=255) ggCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGt > 1:1885130/1‑71 (MQ=255) ggCAATCATCTTATTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTgg < 1:1281114/60‑1 (MQ=255) cAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGtt > 1:1920157/1‑70 (MQ=255) aaTCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTaa < 1:3274092/71‑1 (MQ=255) atcatcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAAc < 1:3632495/71‑1 (MQ=255) tcatcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTc < 1:2660929/49‑1 (MQ=255) tcatcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTaa > 1:1748097/1‑61 (MQ=255) tcatcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTaa > 1:1083874/1‑61 (MQ=255) tcatcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTaa > 1:3112414/1‑61 (MQ=255) catcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACAt > 1:3893112/1‑71 (MQ=255) atcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGaa > 1:1314338/1‑37 (MQ=255) atcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAata > 1:2932235/1‑39 (MQ=255) atcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAata > 1:2450057/1‑39 (MQ=255) atcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAata > 1:354609/1‑39 (MQ=255) atcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTaa > 1:2146109/1‑59 (MQ=255) atcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTaa > 1:1497749/1‑59 (MQ=255) atcTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGGTAACTGGTTAACATc < 1:3306192/71‑1 (MQ=255) cTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATc < 1:3603459/39‑1 (MQ=255) ttGTTCGGCAAACCCTGGGCAATCAGCTTGAGAata > 1:3102834/1‑36 (MQ=255) ttGTTCGGCAAACCCTGGGCAATCAGCTTGAGAata > 1:1462625/1‑36 (MQ=255) ttGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATc > 1:2211201/1‑38 (MQ=255) ttGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATcgcg > 1:2626996/1‑71 (MQ=255) ttGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATcgcg > 1:1191117/1‑71 (MQ=255) ttGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATcgcg > 1:3292245/1‑71 (MQ=255) gTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACa < 1:2190546/64‑1 (MQ=255) | TCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCG > minE/826920‑827058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |