| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 857,256 | A→C | 10.9% | D38E (GAT→GAG) | trpB ← | tryptophan synthase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 857,256 | 0 | A | C | 10.9% | 139.9 / 17.8 | 64 | D38E (GAT→GAG) | trpB | tryptophan synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (24/33); new base C (3/4); total (27/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTA > minE/857189‑857324 | tGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATc < 1:52274/70‑1 (MQ=255) gTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTGGTTGAACTGAGCCTGAAATTCAGGATCttt < 1:1353749/71‑1 (MQ=255) gTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCttt < 1:718769/71‑1 (MQ=255) gTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCtt < 1:3134973/70‑1 (MQ=255) aGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTg > 1:2809623/1‑71 (MQ=255) aGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGCATCTTTTTg < 1:1697111/71‑1 (MQ=255) cgcgGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTgcg > 1:3380647/1‑71 (MQ=255) cgcgGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTgcg > 1:1386844/1‑71 (MQ=255) gcgGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTgcg < 1:3858878/70‑1 (MQ=255) ggACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCtttt > 1:3325947/1‑60 (MQ=255) gACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACa < 1:73897/71‑1 (MQ=255) gCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGcttc > 1:67241/1‑71 (MQ=255) gCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGcttc > 1:3604625/1‑71 (MQ=255) aTAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGcttctt < 1:2860671/71‑1 (MQ=255) tAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTg < 1:3623178/50‑1 (MQ=255) gTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCttcttc < 1:2916301/69‑1 (MQ=255) gTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCttcttc > 1:3279430/1‑69 (MQ=255) gTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCttcttc > 1:13672/1‑69 (MQ=255) gTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCttcttc < 1:1316591/69‑1 (MQ=255) tttttCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCt < 1:1125253/42‑1 (MQ=255) tttttCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCt < 1:2901507/42‑1 (MQ=255) tttttCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCt < 1:263118/42‑1 (MQ=255) tttttCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCt < 1:667592/42‑1 (MQ=255) tttCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCt > 1:2377339/1‑71 (MQ=255) agGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCag < 1:1907357/71‑1 (MQ=255) tCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGc < 1:3680917/71‑1 (MQ=255) ttGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCag < 1:2433706/65‑1 (MQ=255) ttGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAgg > 1:274662/1‑71 (MQ=255) ttGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAgg > 1:1953785/1‑71 (MQ=255) tGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAgg > 1:830004/1‑70 (MQ=255) gAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGc > 1:2893877/1‑70 (MQ=255) gAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCa > 1:3625438/1‑71 (MQ=255) gAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCa > 1:3403320/1‑71 (MQ=255) aaCTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCAt < 1:2909932/71‑1 (MQ=255) aaCTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCAt < 1:1329657/71‑1 (MQ=255) aCTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCAt > 1:3189439/1‑70 (MQ=255) aCTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCAt > 1:390938/1‑70 (MQ=255) cTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCa > 1:2810026/1‑64 (MQ=255) aGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAg < 1:327363/69‑1 (MQ=255) gCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGAt < 1:2843103/71‑1 (MQ=255) ccTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGAtt < 1:2851977/71‑1 (MQ=255) ccTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGAtt < 1:614543/71‑1 (MQ=255) tGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCAt < 1:2431834/62‑1 (MQ=255) tGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCAt < 1:1920950/62‑1 (MQ=255) gAATTTCAGGCTCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAg < 1:1252039/64‑1 (MQ=255) gAATTTCAGGCTCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAg < 1:1819378/64‑1 (MQ=255) gAATTTCAGGCTCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAg < 1:703896/64‑1 (MQ=255) gAATTTCAGGCTCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAg < 1:2057816/64‑1 (MQ=255) gAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTtg < 1:1915203/70‑1 (MQ=255) gAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCAAGCTGGCGCAGAGCAGGCATCAGGATTtg < 1:3708969/70‑1 (MQ=255) aaaTTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTtgtg > 1:2678976/1‑71 (MQ=255) aaaTTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTtgtg > 1:3153576/1‑71 (MQ=255) aaaTTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTtg < 1:689077/69‑1 (MQ=255) atttCAGGCTCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGa > 1:3731702/3‑64 (MQ=255) atttCAGGCTCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGa > 1:2061563/3‑64 (MQ=255) atttCAGGCTCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGa > 1:335390/3‑64 (MQ=255) aaTTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTtgtg > 1:2417554/1‑70 (MQ=255) aTTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTgg < 1:3497246/70‑1 (MQ=255) aTTCAGGATCTTTTTGCGCACTGACAAAAGCTTATTCCAGCTGGCGCaga > 1:2929733/1‑50 (MQ=255) ttCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCa > 1:3594557/1‑71 (MQ=255) cAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTtgt > 1:3542889/1‑65 (MQ=255) aGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGt < 1:2182044/71‑1 (MQ=255) aGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGt < 1:3223019/71‑1 (MQ=255) ggATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTa < 1:2510068/71‑1 (MQ=255) | TGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTA > minE/857189‑857324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |