| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,047,174 | G→A | 24.1% | I40I (ATC→ATT) | ydhH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,047,174 | 0 | G | A | 24.1% | 113.3 / 34.9 | 58 | I40I (ATC→ATT) | ydhH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (28/16); new base A (8/6); total (36/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
CAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACA > minE/1047107‑1047243 | cAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGAATc > 1:1780844/1‑71 (MQ=255) aCTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGc > 1:1326641/1‑71 (MQ=255) cTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCgg > 1:276077/1‑69 (MQ=255) cTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGc > 1:2007939/1‑70 (MQ=255) cTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGcc < 1:1214565/71‑1 (MQ=255) aaGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCa < 1:2842943/71‑1 (MQ=255) aaGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCa < 1:3460200/71‑1 (MQ=255) tgtAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCggg < 1:3907244/55‑1 (MQ=255) tgtAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAAc < 1:2813595/71‑1 (MQ=255) tgtAAGCTGCTGGCCCTGACAAATATCCAGTACAGCCTGTTTCAGAGATACCggg < 1:1530173/55‑1 (MQ=255) gtAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCgg < 1:1982997/59‑1 (MQ=255) gtAAGCTGCTGGCCCTGGCAAATATCCAGTACAGACTGTTTCAGAGATACCGGGATCgg < 1:2511669/59‑1 (MQ=255) tAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACtt < 1:794279/71‑1 (MQ=255) aaGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGcc > 1:341170/1‑59 (MQ=255) aGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACt < 1:2711617/68‑1 (MQ=255) aGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGc > 1:3291858/1‑71 (MQ=255) aGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGc > 1:27738/1‑71 (MQ=255) aGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGc > 1:2288798/1‑71 (MQ=255) aGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGc > 1:818605/1‑71 (MQ=255) gctgctGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGc > 1:2464201/1‑70 (MQ=255) gctgctGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGc > 1:1881296/1‑70 (MQ=255) ctgGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCaa > 1:2268431/1‑55 (MQ=255) ctgGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAg < 1:158209/69‑1 (MQ=255) ctgGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAg < 1:1203/69‑1 (MQ=255) tgGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCaaa > 1:2471228/1‑60 (MQ=255) tGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCggg < 1:2826918/39‑1 (MQ=255) tGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCggg < 1:3868437/39‑1 (MQ=255) gCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGc > 1:892638/1‑57 (MQ=255) gCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGc > 1:2856720/1‑57 (MQ=255) aaaTATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCaaa > 1:3053385/1‑50 (MQ=255) aaTATCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGgt < 1:3245072/71‑1 (MQ=255) aaTATCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGgt < 1:2589934/71‑1 (MQ=255) atatCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAAc > 1:2008863/1‑49 (MQ=255) atatCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGgtg < 1:1330399/71‑1 (MQ=255) atatCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGgtg > 1:1553009/1‑71 (MQ=255) atatCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGgt > 1:2137634/1‑70 (MQ=255) tatCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGgtgt < 1:1946532/71‑1 (MQ=255) tatCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGgtgt < 1:2361683/71‑1 (MQ=255) tatCCAGTACAGCCTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGgtgt < 1:2263040/71‑1 (MQ=255) tCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGtt > 1:3055477/1‑70 (MQ=255) tCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGtt > 1:1410306/1‑70 (MQ=255) ccAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGgtgt > 1:1030699/1‑68 (MQ=255) ccAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTtca > 1:2546778/1‑71 (MQ=255) ccAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTtca > 1:2715944/1‑71 (MQ=255) cAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTtcat > 1:2784734/1‑71 (MQ=255) cAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTtcat > 1:1134923/1‑71 (MQ=255) gTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTtcatca < 1:3228269/71‑1 (MQ=255) tACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCaaa < 1:3676942/40‑1 (MQ=255) cAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAACTGTGCGACCCGGTGTTCATCAAtt > 1:1116639/1‑71 (MQ=255) tttcTGTTTCAGAGATACCGGAATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTg < 1:2864966/68‑1 (MQ=255) aGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTg > 1:2554519/1‑71 (MQ=255) aGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTg > 1:2029668/1‑71 (MQ=255) aGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCAAGCTGTGCGACCCGGTGTTAATCAATTg > 1:3597045/1‑71 (MQ=255) cTGTTTCAGAGATACCGGGATCGGCCAACTCAAACtt < 1:2397033/37‑1 (MQ=255) cTGTTTCAGAGATACCGGGATCGGCCAACTCAAACtt < 1:3352316/37‑1 (MQ=255) cTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCg > 1:883677/1‑71 (MQ=255) tGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGc > 1:1280520/1‑71 (MQ=255) cAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCcaaca > 1:1341258/1‑71 (MQ=255) cAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCcaaca > 1:3895271/1‑71 (MQ=255) agagATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCcaaca > 1:1512651/1‑70 (MQ=255) aTACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCcaacacaaca > 1:3708977/1‑71 (MQ=255) aCCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCcaacacaa < 1:816834/67‑1 (MQ=255) gggATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCaa > 1:340242/1‑70 (MQ=255) gggATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCaa > 1:2337352/1‑70 (MQ=255) gggATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCaa > 1:83919/1‑70 (MQ=255) gggATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCaa > 1:18254/1‑70 (MQ=255) gggATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGCTGTTCATCAATTGTCGCCAACACAACATCAac < 1:2789035/71‑1 (MQ=255) ggATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCcaacacaa > 1:117311/1‑63 (MQ=255) ggATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAaca < 1:3889224/71‑1 (MQ=255) ggATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAaca > 1:427661/1‑71 (MQ=255) | CAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACA > minE/1047107‑1047243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |