Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,125,754 | C→T | 8.2% | A294T (GCC→ACC) | pheS ← | phenylalanine tRNA synthetase, alpha subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,125,754 | 0 | C | T | 8.2% | 229.8 / 8.9 | 73 | A294T (GCC→ACC) | pheS | phenylalanine tRNA synthetase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (34/33); new base T (2/4); total (36/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 |
CGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCATCCCG > minE/1125685‑1125818 | cGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGc < 1:237916/70‑1 (MQ=255) aTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGTGAAACCa < 1:3456110/71‑1 (MQ=255) aTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:3769093/71‑1 (MQ=255) aTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:3561657/71‑1 (MQ=255) tGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCag < 1:1023552/71‑1 (MQ=255) tGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCag < 1:796821/71‑1 (MQ=255) aCGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCTAAACCagag > 1:3263031/1‑70 (MQ=255) aCGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAAGCAGAGt < 1:727/71‑1 (MQ=255) aCGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCagag > 1:757022/1‑70 (MQ=255) cGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGACTa < 1:2350776/71‑1 (MQ=255) aGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTaa > 1:781505/1‑69 (MQ=255) tCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTc < 1:3333122/71‑1 (MQ=255) cGGTCACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaaa > 1:3507934/1‑56 (MQ=255) ggTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCTTCCCGAAGGCGAAACCAGAGTAAACTTcc < 1:647437/70‑1 (MQ=255) ggTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTcc < 1:3048488/70‑1 (MQ=255) ggTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCg > 1:1547924/1‑71 (MQ=255) ggTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCg > 1:1356695/1‑71 (MQ=255) gTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCgg < 1:3039758/71‑1 (MQ=255) aCGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGTGAAACCAGAGTAAACTTCCGGGTc < 1:2386924/71‑1 (MQ=255) aCGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTc < 1:1996570/71‑1 (MQ=255) gCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGa < 1:1000120/71‑1 (MQ=255) gCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGa < 1:1070330/71‑1 (MQ=255) gCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGa < 1:2227245/71‑1 (MQ=255) ccGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTa < 1:2017155/56‑1 (MQ=255) cGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:127502/50‑1 (MQ=255) cGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATg > 1:2359973/1‑71 (MQ=255) gCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACg > 1:3588549/1‑71 (MQ=255) cAACATAGTCAGACGCTCCATCCCCATCCCGAAGTCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGt < 1:2924199/71‑1 (MQ=255) cAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGt < 1:2497240/71‑1 (MQ=255) aTAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACg > 1:2525025/1‑71 (MQ=255) tAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:3408405/38‑1 (MQ=255) gTCAGACGCTCCATCCCCATCCCGACGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCa < 1:2688737/70‑1 (MQ=255) gTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCa < 1:2328360/70‑1 (MQ=255) aGACGCTCCATCCCCATCCCGAAGGTGAAACCAGAGTAAACTTCCggg > 1:2307966/1‑48 (MQ=255) aGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAacac > 1:3876163/1‑71 (MQ=255) aGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAacac > 1:2939741/1‑71 (MQ=255) gACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACg < 1:1042582/71‑1 (MQ=255) cGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCa > 1:2540988/1‑64 (MQ=255) cTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCg < 1:724606/71‑1 (MQ=255) ccatccccatccCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCaa > 1:3127384/1‑51 (MQ=255) catccccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCTATGCCAACGTTACGCAACACGTTCGGAt < 1:1794574/71‑1 (MQ=255) catccccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGa < 1:3488152/70‑1 (MQ=255) catccccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGa < 1:760300/70‑1 (MQ=255) catccccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGAt > 1:3664721/1‑71 (MQ=255) tccccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGc < 1:1514064/56‑1 (MQ=255) ccccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCa > 1:2401950/1‑71 (MQ=255) ccccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCa > 1:3990082/1‑71 (MQ=255) ccccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCa > 1:491151/1‑71 (MQ=255) ccccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCa > 1:2570021/1‑71 (MQ=255) cccatcccGAAGGTGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTAGGATGCAc > 1:3465058/1‑71 (MQ=255) cccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTGGGAt > 1:3651304/1‑67 (MQ=255) cccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCa < 1:1950097/70‑1 (MQ=255) cccatcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCa < 1:3880197/70‑1 (MQ=255) atcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCAt > 1:656985/1‑71 (MQ=255) atcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCAt > 1:3166429/1‑71 (MQ=255) atcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCAt > 1:2171650/1‑71 (MQ=255) ataccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCAt > 1:3463903/4‑71 (MQ=255) tcccGAAGGTGAAACCAGAGTAAACTTCCGGGTCGATGCCAAc < 1:3286892/43‑1 (MQ=255) tcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATga > 1:787099/1‑38 (MQ=255) tcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCAt < 1:2002800/70‑1 (MQ=255) tcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCAt < 1:2007551/70‑1 (MQ=255) tcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCATc > 1:2240198/1‑71 (MQ=255) tcccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCATc > 1:567011/1‑71 (MQ=255) ccGAAGGTGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCATccc < 1:2681396/71‑1 (MQ=255) ccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGtt > 1:2665135/1‑56 (MQ=255) ccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCATcc > 1:3578573/1‑70 (MQ=255) ccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCATcc > 1:2905231/1‑70 (MQ=255) ccGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCATc > 1:2324052/1‑69 (MQ=255) acGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGc > 1:1474624/2‑48 (MQ=255) gAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCaa < 1:237353/48‑1 (MQ=255) aaGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCaa > 1:1756843/1‑47 (MQ=255) aaGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCaa > 1:2951798/1‑47 (MQ=255) aaGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCg < 1:1973378/55‑1 (MQ=255) aaGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCATCCCg > 1:2152374/1‑69 (MQ=255) | CGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGTTACGCAACACGTTCGGATGCACCATCCCG > minE/1125685‑1125818 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |