| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 29,160 | T→C | 6.7% | R222R (CGT→CGC) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 29,160 | 0 | T | C | 6.7% | 239.1 / 9.2 | 89 | R222R (CGT→CGC) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (29/54); new base C (4/2); total (33/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01 | |||||||||||
CGACCAAAGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGG > minE/29093‑29228 | cGACCAAAGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGa > 1:437485/1‑70 (MQ=255)cGACCAAAGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGa > 1:361581/1‑70 (MQ=255)cGACCAAAGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGAt < 1:3390540/71‑1 (MQ=255)cGACCAAAGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGAt < 1:1854292/71‑1 (MQ=255)cGACCAAAGAGTGGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGa > 1:281821/1‑70 (MQ=255) cAAAGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATaaaa < 1:2083262/71‑1 (MQ=255) cAAAGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATaaaa < 1:3348318/71‑1 (MQ=255) cAAAGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATaaa > 1:3231441/1‑70 (MQ=255) aaGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATaaaa < 1:566721/69‑1 (MQ=255) aaGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAAc < 1:1340825/71‑1 (MQ=255) aaGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAAc < 1:1370709/71‑1 (MQ=255) gagTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGa > 1:6795/1‑71 (MQ=255) gTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACa < 1:1411020/71‑1 (MQ=255) cTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGAc < 1:3319799/66‑1 (MQ=255) tgattgatGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACa < 1:2229300/66‑1 (MQ=255) tgattgatGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACa < 1:163292/66‑1 (MQ=255) attgatGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTTATAAAAACGACAACTGc < 1:3742618/69‑1 (MQ=255) attgatGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACaa > 1:1420460/1‑65 (MQ=255) tgatgaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAAc < 1:3317612/58‑1 (MQ=255) tgatgaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGAc > 1:2471959/1‑61 (MQ=255) tgatgaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGGGGTGCGTGATAAAAAc < 1:1353721/58‑1 (MQ=255) gatgaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGcatcat > 1:2476285/1‑71 (MQ=255) gatgaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGcatcat > 1:2212175/1‑71 (MQ=255) gatgaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGcatcat > 1:3982518/1‑71 (MQ=255) gaagaGTCGCTGCTGGGCTGGAAAGAGTACGAGATGGAAGTCGTTCGCGACAAGAACGACAACTGca < 1:1682420/64‑1 (MQ=255) gaagaGTCGCTGCTGGGCTGGAAAGAGTACGAGATGGAAGTCGTTCGCGACAAGAACGACAAATGca < 1:3391729/64‑1 (MQ=255) atgaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGa > 1:657205/1‑58 (MQ=255) tgaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCg < 1:1808405/71‑1 (MQ=255) agaGTCGCTGCTGGGCTGGAAAGAGTACGAGATGGAAGTCGTTCGCGACAAGAACGACAACTGcatc > 1:384156/2‑67 (MQ=255) agaGTCGCTGCTGGGCTGGAAAGAGTACGAGATGGAAGTCGTTCGCGACAAGAACGACAACTGcatc > 1:1019702/2‑67 (MQ=255) gaGTCGCTGATCGGCTGGAACGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCg < 1:1504168/70‑1 (MQ=255) gTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCt > 1:2103628/1‑71 (MQ=255) gTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCt > 1:1069807/1‑71 (MQ=255) gTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCt > 1:1875249/1‑71 (MQ=255) gCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATaaa < 1:3196388/46‑1 (MQ=255) gCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACaa > 1:1969373/1‑54 (MQ=255) aTCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGAc < 1:192789/48‑1 (MQ=255) aTCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTa < 1:702284/70‑1 (MQ=255) aTCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTAt > 1:2058862/1‑71 (MQ=255) ggCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATaaaaa > 1:2972137/1‑41 (MQ=255) ggCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATaaaaa > 1:422497/1‑41 (MQ=255) ggCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCg < 1:3962498/70‑1 (MQ=255) ggCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCg < 1:1222667/70‑1 (MQ=255) ggCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCg < 1:2626890/70‑1 (MQ=255) gCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGaa < 1:706362/71‑1 (MQ=255) cTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTc > 1:939821/1‑58 (MQ=255) tGGAAAGAGTACGAGATGGAAGTGGTGCGTGATaaa < 1:1245005/36‑1 (MQ=255) ggAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGct < 1:2608360/60‑1 (MQ=255) aaGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGt > 1:3152400/1‑52 (MQ=255) aaGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTc > 1:213725/1‑53 (MQ=255) aaGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTc < 1:280784/71‑1 (MQ=255) aaGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTc < 1:2481305/71‑1 (MQ=255) aaGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTc < 1:893095/71‑1 (MQ=255) agagTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcg < 1:1946519/71‑1 (MQ=255) agagTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTc > 1:3073476/1‑70 (MQ=255) agTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgat > 1:1266611/1‑71 (MQ=255) agTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgat > 1:2310295/1‑71 (MQ=255) tccGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgc < 1:1511192/69‑1 (MQ=255) aCGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgc < 1:983501/70‑1 (MQ=255) aCGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgc < 1:2066933/70‑1 (MQ=255) cGAGATTGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcga < 1:1265662/71‑1 (MQ=255) cGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcga < 1:570091/71‑1 (MQ=255) gagaTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgat < 1:1538002/71‑1 (MQ=255) gagaTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgat < 1:3920045/71‑1 (MQ=255) gagaTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgat < 1:3349105/71‑1 (MQ=255) agaTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgatg > 1:1206870/1‑71 (MQ=255) gaTTGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgat < 1:194416/69‑1 (MQ=255) gaTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATgg < 1:3567857/71‑1 (MQ=255) gaTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATgg < 1:2166276/71‑1 (MQ=255) gaTGGAAGTCGTTCGCGACAAGAACGACAACTGCATCATCATCTGCTCGATCGAGAACCTggatccga > 1:2434487/1‑68 (MQ=255) gaTGGAAGTCGTTCGCGACAAGAACGACAACTGCATCATCATCTGCTCGATCAAGAACCTggatccga > 1:792767/1‑68 (MQ=255) aTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATgg < 1:2171051/70‑1 (MQ=255) ggAAGTGGTGCTTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgat < 1:560068/66‑1 (MQ=255) ggAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCa > 1:2935347/1‑71 (MQ=255) aaGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATc > 1:3774596/1‑71 (MQ=255) aaGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATc < 1:1451196/71‑1 (MQ=255) aGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTc < 1:2943821/55‑1 (MQ=255) tggtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgat < 1:3791995/61‑1 (MQ=255) tggtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgat < 1:3520752/61‑1 (MQ=255) tggtgCGTGATAAAAACCACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgat < 1:1179256/61‑1 (MQ=255) ggtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgat < 1:2983477/60‑1 (MQ=255) gtgCGTGATAAAAACGACAACTGCATCATCGTCTTCTCTATCGAAAACTTcgatgcgat < 1:659271/59‑1 (MQ=255) gtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCacac < 1:1055805/71‑1 (MQ=255) gtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCacac < 1:1955758/71‑1 (MQ=255) gtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCacac < 1:2972940/71‑1 (MQ=255) cGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACt < 1:3742816/46‑1 (MQ=255) cGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACt < 1:2589048/46‑1 (MQ=255) cGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTcgatgcgatg > 1:4003132/1‑57 (MQ=255) cGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATgg > 1:1295749/1‑58 (MQ=255) tGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTctatgc < 1:2934962/51‑1 (MQ=255) tGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCgg < 1:3371505/69‑1 (MQ=255) | CGACCAAAGAGTTGCTGATTGATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGG > minE/29093‑29228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |