| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,150,016 | G→A | 12.5% | intergenic (‑69/‑133) | osmE ← / → nadE | DNA‑binding transcriptional regulator/NAD synthetase, NH3/glutamine‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,150,016 | 0 | G | A | 12.5% | 206.9 / 17.7 | 72 | intergenic (‑69/‑133) | osmE/nadE | DNA‑binding transcriptional regulator/NAD synthetase, NH3/glutamine‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (29/34); new base A (4/5); total (33/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
ATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCCGCTGTCGC > minE/1149946‑1150083 | atatTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTg < 1:1650394/71‑1 (MQ=255) tatTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGa < 1:4035270/71‑1 (MQ=255) tatTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGa < 1:1958026/71‑1 (MQ=255) tatTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGa < 1:1906565/71‑1 (MQ=255) cGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGa < 1:826042/70‑1 (MQ=255) cGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGa < 1:800124/70‑1 (MQ=255) cGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGa < 1:3056412/70‑1 (MQ=255) cGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTAAACGa < 1:768539/70‑1 (MQ=255) cGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTAAACGa < 1:1805650/70‑1 (MQ=255) ttGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTc > 1:3500672/1‑71 (MQ=255) ttGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTc > 1:1598951/1‑71 (MQ=255) ttGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTc > 1:2848332/1‑71 (MQ=255) ttGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTc > 1:4121582/1‑71 (MQ=255) ttGTTTATCAGCGTGTTAGATAAGACTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTc > 1:3452094/1‑71 (MQ=255) gTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCt < 1:2950792/68‑1 (MQ=255) gTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCt < 1:2230518/68‑1 (MQ=255) gTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTAAACGAAACGGCTcc > 1:2269093/1‑70 (MQ=255) aTCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCtt < 1:1741849/70‑1 (MQ=255) aTCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTAAACGAAACGGCTCCGCtt < 1:3322190/70‑1 (MQ=255) tCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTc < 1:2105183/71‑1 (MQ=255) aGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTAAACGAAACGGCTCCGCTTTCag < 1:3321537/71‑1 (MQ=255) cGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCagag < 1:14268/71‑1 (MQ=255) cGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCagag < 1:1100035/71‑1 (MQ=255) gtgtTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCagag < 1:3155620/70‑1 (MQ=255) tgtTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGa > 1:3880746/1‑71 (MQ=255) tgtTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGa > 1:2751335/1‑71 (MQ=255) ttAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGAtt > 1:3388296/1‑71 (MQ=255) ttAGATAAGCCTGGAATACATTGGGAGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGAt < 1:588243/70‑1 (MQ=255) aGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTcc > 1:1683322/1‑71 (MQ=255) aGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTAAACGAAACGGCTCCGCTTTCAGAGGATTcc > 1:2033397/1‑71 (MQ=255) aGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTAAACGAAACGGCTCCGCTTTCAGAGGATTcc > 1:1931634/1‑71 (MQ=255) gATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTcc < 1:3646437/70‑1 (MQ=255) tAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGa < 1:436191/43‑1 (MQ=255) tAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCagag > 1:1448937/1‑62 (MQ=255) tAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTg < 1:1645037/70‑1 (MQ=255) aaGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGaaa > 1:3387741/1‑44 (MQ=255) aGCCTGGACTACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTa < 1:761777/70‑1 (MQ=255) aGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGaaa > 1:1058952/1‑43 (MQ=255) aGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGaaa > 1:476593/1‑43 (MQ=255) aGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGaa > 1:1601678/1‑42 (MQ=255) aGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGt > 1:2798072/1‑69 (MQ=255) aGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTAt > 1:2802723/1‑71 (MQ=255) aGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTAt > 1:2331211/1‑71 (MQ=255) cTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGAc > 1:1373791/1‑71 (MQ=255) cTGGAATACATTGGGCGCTTTTTCAAGCCCGTAAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGa < 1:4107948/70‑1 (MQ=255) tGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCaga > 1:3652661/1‑55 (MQ=255) aaTACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGttt > 1:2648958/1‑71 (MQ=255) tACATTGGGCGCTTTTTCAAGCCCGTAAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGtttt > 1:3816861/1‑70 (MQ=255) cATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGAt < 1:1134811/52‑1 (MQ=255) cATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTaa < 1:2371755/70‑1 (MQ=255) ttGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCt < 1:1308857/54‑1 (MQ=255) ttGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTATATGACg > 1:2103543/1‑62 (MQ=255) gcTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTc < 1:2617879/71‑1 (MQ=255) gcTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTc < 1:1296087/71‑1 (MQ=255) cTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCt < 1:3459444/47‑1 (MQ=255) tttttCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTg < 1:3763264/47‑1 (MQ=255) cttttCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAg < 1:16824/70‑1 (MQ=255) ttttCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTg > 1:941649/1‑46 (MQ=255) ttttCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGt > 1:2851489/1‑47 (MQ=255) tttCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCt < 1:1230409/44‑1 (MQ=255) tttCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGc < 1:3626994/70‑1 (MQ=255) tttCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGc < 1:151137/70‑1 (MQ=255) tCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGccc < 1:3924459/70‑1 (MQ=255) tCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCCg > 1:3042423/1‑71 (MQ=255) tCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCCg > 1:1101593/1‑71 (MQ=255) aaGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCAt > 1:2766189/1‑61 (MQ=255) aGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCCGCTg > 1:2293589/1‑71 (MQ=255) gCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCAGCTGt > 1:761604/1‑71 (MQ=255) cccGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCCGCTGTc < 1:1310523/71‑1 (MQ=255) ccGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCCGCTGTc < 1:1282833/70‑1 (MQ=255) cGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCCGCTGTCg > 1:2747054/1‑70 (MQ=255) cGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCCGCTGTCGc < 1:2079487/71‑1 (MQ=255) tGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCAtt < 1:3657891/55‑1 (MQ=255) | ATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATACATTGGGCGCTTTTTCAAGCCCGTGAACGAAACGGCTCCGCTTTCAGAGGATTCCTGTATGACGTTTTAACCACCATTCAGCCCGCTGTCGC > minE/1149946‑1150083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |