| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,380,514 | T→C | 6.2% | I62V (ATT→GTT) | galS ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,380,514 | 0 | T | C | 6.2% | 271.7 / 9.0 | 96 | I62V (ATT→GTT) | galS | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (56/34); new base C (4/2); total (60/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
CGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGGC > minE/1380445‑1380583 | cGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATg < 1:1529438/71‑1 (MQ=255) aCCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGgt < 1:3323079/71‑1 (MQ=255) ccAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGgtg > 1:2861972/1‑71 (MQ=255) cAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGgtgt < 1:3876222/71‑1 (MQ=255) aTCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGc < 1:144450/71‑1 (MQ=255) tCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCt < 1:78155/71‑1 (MQ=255) tCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCt < 1:340511/71‑1 (MQ=255) ccACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCt > 1:3082106/1‑70 (MQ=255) ccACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCt > 1:1345755/1‑70 (MQ=255) cACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTa < 1:1118771/70‑1 (MQ=255) aCCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAAt < 1:1538942/59‑1 (MQ=255) aCCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAAt < 1:1493268/59‑1 (MQ=255) aCCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAAt < 1:819156/59‑1 (MQ=255) aCCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAAc > 1:2510953/1‑71 (MQ=255) aCCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAAc > 1:451468/1‑71 (MQ=255) aCCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAACGGTGTCGCTAAc > 1:1449831/1‑71 (MQ=255) ccGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAAt < 1:509703/58‑1 (MQ=255) ccGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAAcc > 1:2277808/1‑71 (MQ=255) cGCTTTTACCAGCGCGCCGAAAACCGCATCAGAAACGTCCATCACCACCACGCCAATgg > 1:1415747/1‑59 (MQ=255) cGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAAt < 1:2119658/57‑1 (MQ=255) cGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATgg > 1:3734788/1‑59 (MQ=255) cGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAAcc > 1:3678842/1‑70 (MQ=255) cGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCt > 1:1652953/1‑71 (MQ=255) gCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGc > 1:3162143/1‑64 (MQ=255) gCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGc > 1:2534827/1‑64 (MQ=255) gCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCt > 1:3188286/1‑70 (MQ=255) gCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCt > 1:2415583/1‑70 (MQ=255) cTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAACGGTGTCGCTAACCt > 1:1547811/1‑69 (MQ=255) ttttACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGa > 1:3647957/1‑70 (MQ=255) ttttACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAg > 1:3450248/1‑71 (MQ=255) ttACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAg < 1:293368/69‑1 (MQ=255) ttACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGtt > 1:3261967/1‑71 (MQ=255) aCCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTg < 1:3338812/70‑1 (MQ=255) aCCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTg < 1:418751/70‑1 (MQ=255) aCCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTg < 1:1321105/70‑1 (MQ=255) aCCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTg < 1:3667928/70‑1 (MQ=255) ccAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTg < 1:2795531/69‑1 (MQ=255) ccAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTg < 1:1113560/69‑1 (MQ=255) cAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCa < 1:122323/71‑1 (MQ=255) aGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCa < 1:3878764/70‑1 (MQ=255) gcgcCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAgcgc > 1:2085139/1‑71 (MQ=255) gcCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCt < 1:1631918/51‑1 (MQ=255) cGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTgg > 1:3524864/1‑71 (MQ=255) cGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTgg > 1:1239339/1‑71 (MQ=255) cGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTgg < 1:3134130/71‑1 (MQ=255) gAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGc > 1:2914784/1‑60 (MQ=255) gAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGc > 1:1306459/1‑60 (MQ=255) gAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTggg < 1:1867420/71‑1 (MQ=255) gAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTggg < 1:2563011/71‑1 (MQ=255) aaaaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGa < 1:1353311/54‑1 (MQ=255) aaaaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGa < 1:3363976/54‑1 (MQ=255) aaaaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGa < 1:1417634/54‑1 (MQ=255) aaaaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTggg > 1:3861442/1‑70 (MQ=255) aaaaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTggg > 1:2243334/1‑70 (MQ=255) aaaaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTgg > 1:2228278/1‑69 (MQ=255) aaaaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTgg > 1:645921/1‑69 (MQ=255) aaaaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGc < 1:1284333/71‑1 (MQ=255) aaaaaCGCATAAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTgg > 1:1757978/1‑69 (MQ=255) aaaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGc < 1:2712998/70‑1 (MQ=255) aaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGt > 1:1452194/1‑54 (MQ=255) aaaCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGt > 1:1281762/1‑54 (MQ=255) aaaCGCATCAGAAACGTCCATCACCACCACGCCAACGGTGTCGCTAACCTGAGt > 1:1539711/1‑54 (MQ=255) aaaCGCATCAGAAACGTCCATCACCACCACGCCAACGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCAt < 1:3279358/71‑1 (MQ=255) tCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGtt < 1:3082334/48‑1 (MQ=255) tCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGtt < 1:1978126/48‑1 (MQ=255) tCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGtt > 1:1197879/1‑71 (MQ=255) tCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGtt > 1:2880400/1‑71 (MQ=255) cAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCTTGGGCATTGGCGttt > 1:3300657/1‑71 (MQ=255) aGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGttt > 1:447064/1‑70 (MQ=255) aGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGttt > 1:2029887/1‑70 (MQ=255) aGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTg > 1:3239803/1‑71 (MQ=255) aGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTg > 1:776098/1‑71 (MQ=255) aGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTg > 1:1375566/1‑71 (MQ=255) aGAAACGTCCATCACCACCACGCCAATGGTGACGCTAACCTGAGTTGCCAgcg > 1:917456/1‑53 (MQ=255) aGAAACGTCCATCACCACCACGCCAACGGTGTCGCTAACCTg < 1:1554834/42‑1 (MQ=255) aaaCGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCg < 1:3611713/65‑1 (MQ=255) aCGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGcc > 1:1420653/1‑70 (MQ=255) gTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGa < 1:1618188/70‑1 (MQ=255) tCCATCACCACCACGCCAATGGTGTCGCTAACCTGAg < 1:1246214/37‑1 (MQ=255) ccATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATaa > 1:3513826/1‑71 (MQ=255) aTCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATc > 1:3327962/1‑71 (MQ=255) aTCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATc < 1:1971869/71‑1 (MQ=255) tCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATc > 1:4060601/1‑70 (MQ=255) caccaccacGCCAACGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATc > 1:1514450/1‑69 (MQ=255) accaccacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTg > 1:1861122/1‑58 (MQ=255) accaccacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCa > 1:3731088/1‑70 (MQ=255) accaccacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAg > 1:1007836/1‑71 (MQ=255) accaccacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAg > 1:1329298/1‑71 (MQ=255) accaccacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAg > 1:749601/1‑71 (MQ=255) accaccacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAg > 1:1376920/1‑71 (MQ=255) ccaccacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATc > 1:2631229/1‑67 (MQ=255) caccacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGct < 1:384904/71‑1 (MQ=255) accacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGc > 1:2042626/1‑69 (MQ=255) cacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCa < 1:2668495/65‑1 (MQ=255) cGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGctcact > 1:227239/1‑70 (MQ=255) cGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGctcact > 1:2022779/1‑70 (MQ=255) ccAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGctcactca > 1:2786718/1‑70 (MQ=255) aaTGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGAAAATCCAg > 1:2389229/1‑60 (MQ=255) aaTGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGAGTTTGGCCGATAATCCAGCTCACTCACgg > 1:1441046/1‑71 (MQ=255) aTGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGGc < 1:3750986/71‑1 (MQ=255) | CGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGGC > minE/1380445‑1380583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |