| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,970,940 | C→G | 8.0% | E656Q (GAA→CAA) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,970,940 | 0 | C | G | 8.0% | 330.5 / 12.6 | 111 | E656Q (GAA→CAA) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base C (59/43); new base G (3/6); total (62/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
ACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAG > minE/1970871‑1971009 | acaATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc > 1:341898/1‑70 (MQ=255) aaTAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc > 1:3803090/1‑68 (MQ=255) tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGa > 1:17758/1‑70 (MQ=255) tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGAt < 1:3122103/71‑1 (MQ=255) tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGAt < 1:200200/71‑1 (MQ=255) ttGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGt < 1:1362328/71‑1 (MQ=255) ttGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGt < 1:534074/71‑1 (MQ=255) gtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATaa > 1:1588477/1‑67 (MQ=255) gtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGta > 1:3676112/1‑70 (MQ=255) gtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGta > 1:44823/1‑70 (MQ=255) gtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGta > 1:627246/1‑70 (MQ=255) tgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGtat < 1:1750548/70‑1 (MQ=255) gCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATaa < 1:2773499/70‑1 (MQ=255) cGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCaa < 1:738448/58‑1 (MQ=255) ggATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAAcc < 1:529796/70‑1 (MQ=255) taTTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCg > 1:852155/2‑70 (MQ=255) gATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGAt < 1:3535888/59‑1 (MQ=255) gATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAAcc > 1:52161/1‑69 (MQ=255) gATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAAc > 1:3070273/1‑68 (MQ=255) gATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCg > 1:2934206/1‑70 (MQ=255) aTTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGAt < 1:2480331/58‑1 (MQ=255) ttATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTa > 1:2197282/1‑70 (MQ=255) ttATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTa > 1:2137229/1‑70 (MQ=255) ttATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTa > 1:2143892/1‑70 (MQ=255) ttATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTa > 1:1169877/1‑70 (MQ=255) ttATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTa > 1:862892/1‑70 (MQ=255) ttATCACCGCTAAATTAAGCGGATTCAAGCAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTa > 1:2856310/1‑70 (MQ=255) tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTGAAGATAAGTATAACCGTTCaa < 1:2714076/70‑1 (MQ=255) tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGtat < 1:1468841/60‑1 (MQ=255) tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACaa < 1:969366/70‑1 (MQ=255) tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACaa < 1:1400826/70‑1 (MQ=255) tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACaa < 1:3733195/70‑1 (MQ=255) cACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAAc > 1:283978/1‑71 (MQ=255) aCCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAAcc < 1:3911257/71‑1 (MQ=255) aCCGCTAAATTAAGCGGATTCAAGTAACAC‑‑GACTTACTCATCTTCAAGATaa > 1:2213445/1‑52 (MQ=255) ccGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCt < 1:871155/71‑1 (MQ=255) ccGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCt < 1:1579716/71‑1 (MQ=255) cGCTAAATTAAGCGGATTCAAGTACCACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCt > 1:3735059/1‑70 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGAtga < 1:1070362/51‑3 (MQ=38) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGAtga < 1:1762797/51‑3 (MQ=38) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACa < 1:3246306/64‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGc > 1:1408354/1‑71 (MQ=255) cTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGcc < 1:205824/71‑1 (MQ=255) aaaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTc > 1:1951648/1‑71 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAAc > 1:2150572/1‑54 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCg < 1:2694898/71‑1 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCg < 1:1519248/71‑1 (MQ=255) aTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTc < 1:2864922/69‑1 (MQ=255) aTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCg < 1:2711706/70‑1 (MQ=255) aTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCg < 1:620801/70‑1 (MQ=255) ttAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACaaa > 1:3868773/1‑60 (MQ=255) ttAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACaaa > 1:1892324/1‑60 (MQ=255) aaGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAct > 1:456728/1‑71 (MQ=255) gCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct < 1:2822201/71‑1 (MQ=255) cGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCtt < 1:2958140/71‑1 (MQ=255) cGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCtt < 1:1013174/71‑1 (MQ=255) gATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc < 1:3810191/70‑1 (MQ=255) gATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc < 1:3081021/70‑1 (MQ=255) gATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc > 1:814379/1‑70 (MQ=255) gATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTCCCTCGAACTCTTc > 1:1184730/1‑70 (MQ=255) aTTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActc > 1:2655417/1‑66 (MQ=255) aTTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc > 1:575903/1‑69 (MQ=255) ttCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAg < 1:1106049/71‑1 (MQ=255) ttCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAg < 1:3620488/71‑1 (MQ=255) tCAAGTAACACAGGACTTACTTATCTTGAAGATGAg > 1:4089161/1‑36 (MQ=18) tCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAg > 1:521913/1‑70 (MQ=255) aGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGcc > 1:38910/1‑53 (MQ=255) tAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtg > 1:1962623/1‑71 (MQ=255) aaCACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAAcc > 1:3282876/1‑46 (MQ=255) acacAGGACTTACTCATCTTCAAGATAAGTATAAc > 1:352935/1‑35 (MQ=255) acacAGGACTTACTCATCTTCAAGATAAGTATAAc > 1:953380/1‑35 (MQ=255) acacAGAACTTACTCATCTTCAAGATAAGTATAAc > 1:9717/1‑35 (MQ=255) aGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGttgttg < 1:700902/71‑1 (MQ=255) aGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGttgttg < 1:2521966/71‑1 (MQ=255) ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGc < 1:1967598/71‑1 (MQ=255) gACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGttgttg > 1:3588070/1‑69 (MQ=255) gACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGttgttg > 1:520884/1‑69 (MQ=255) aCTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAg < 1:592192/71‑1 (MQ=255) aCTTATCTTGAAGATGAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGa < 1:31171/54‑1 (MQ=37) aCTTATCTTGAAGATGAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGa < 1:402953/54‑1 (MQ=37) aCTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgttg > 1:2883665/1‑61 (MQ=255) aCTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgttg > 1:2583895/1‑61 (MQ=255) aCTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGtt > 1:280679/1‑69 (MQ=255) aCTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGtt > 1:4072493/1‑69 (MQ=255) aCTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGtt < 1:2499053/69‑1 (MQ=255) aCTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGtt > 1:1574777/1‑69 (MQ=255) ttaTCTTGAAGATGAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAg > 1:891039/3‑70 (MQ=255) aTCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGaa > 1:2336435/1‑39 (MQ=255) aTCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGaa > 1:3996891/1‑39 (MQ=255) tCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCAt > 1:390647/1‑70 (MQ=255) ttCAAGATAAGTATAACCGTACAAACCTGCCTCGaa > 1:651379/1‑36 (MQ=255) ttCAAGATAAGTATAACCGTACAAACCTGCCTCGaa > 1:1385854/1‑36 (MQ=255) ttCAAGATAAGTATAACCGTACAAACCTGCCTCGaa > 1:2854373/1‑36 (MQ=255) ttCAAGATAAGTATAACCGTACAAACCTGCCTCGaa > 1:2138852/1‑36 (MQ=255) ttCAAGATAAGTATAACCGTACAAACCTGCCTCGaa > 1:2747716/1‑36 (MQ=255) cAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATc > 1:1805667/1‑67 (MQ=255) cAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATc > 1:1396130/1‑67 (MQ=255) cAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATc > 1:3247943/1‑67 (MQ=255) cAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAg < 1:3490540/70‑1 (MQ=255) cAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAg < 1:3394697/70‑1 (MQ=255) | ACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAG > minE/1970871‑1971009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |