| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 204,556 | A→G | 100% | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 204,556 | 0 | A | G | 100.0% | 254.3 / NA | 83 | intergenic (+156/‑28) | alaV/rrlH | tRNA‑Ala/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (40/43); total (40/43) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG > minE/204493‑204622 | aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc > 1:3304023/1‑69 (MQ=255) aTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc > 1:1526498/1‑68 (MQ=255) ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:2422923/71‑1 (MQ=255) tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa > 1:4046475/1‑70 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:1273457/70‑1 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa > 1:1039198/1‑69 (MQ=255) cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc > 1:172190/1‑71 (MQ=255) acTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt < 1:4026390/71‑1 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt < 1:2420792/71‑1 (MQ=255) tGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc > 1:1873355/1‑71 (MQ=255) tGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc > 1:3695661/1‑71 (MQ=255) gAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa < 1:538700/59‑1 (MQ=255) gAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa < 1:2986390/59‑1 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATTATGGATCGCAAGAAAc < 1:1521121/63‑1 (MQ=11) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATGGCAAGAAACATCTTCgg > 1:2045965/1‑71 (MQ=11) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc < 1:1206004/63‑1 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc > 1:98533/1‑69 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg > 1:3168840/1‑71 (MQ=255) acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc < 1:3071554/62‑1 (MQ=255) acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg > 1:2779770/1‑71 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg < 1:2446768/69‑1 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:3461114/3‑71 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:1094180/3‑71 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:1373374/3‑71 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:1835302/3‑71 (MQ=255) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt < 1:1260366/70‑1 (MQ=19) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:792665/2‑71 (MQ=19) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:4067665/1‑57 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:3867685/1‑57 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:487031/1‑57 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:210797/1‑57 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:3455852/1‑57 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:5967/1‑57 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:2712540/1‑57 (MQ=255) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg < 1:1420817/70‑1 (MQ=25) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg < 1:4000367/70‑1 (MQ=25) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg < 1:529846/70‑1 (MQ=25) aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa > 1:1981068/1‑54 (MQ=255) aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa > 1:637433/1‑54 (MQ=255) aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg < 1:3219862/71‑1 (MQ=21) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTa < 1:1647447/70‑1 (MQ=25) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAg < 1:1481574/71‑1 (MQ=21) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAg < 1:792485/71‑1 (MQ=21) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc > 1:2519128/1‑70 (MQ=25) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:3492054/70‑1 (MQ=25) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2316487/70‑1 (MQ=25) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2321356/70‑1 (MQ=25) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc < 1:2110155/48‑1 (MQ=255) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt < 1:452493/64‑1 (MQ=25) gAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc > 1:3232530/1‑70 (MQ=25) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt < 1:3393357/70‑1 (MQ=25) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt < 1:3880628/70‑1 (MQ=25) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt < 1:1891204/70‑1 (MQ=25) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTa < 1:3914009/71‑1 (MQ=21) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:1245486/39‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa < 1:2076676/56‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTa < 1:26412/59‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:260124/1‑71 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:1196250/1‑71 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:805654/1‑71 (MQ=21) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTa < 1:3456961/57‑1 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTa < 1:1450729/57‑1 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:2551494/69‑1 (MQ=25) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:684981/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:95949/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca < 1:3868999/71‑1 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3470387/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca < 1:180224/71‑1 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa < 1:194062/69‑1 (MQ=25) aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacact < 1:3549354/71‑2 (MQ=19) tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:3182648/1‑43 (MQ=255) ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg > 1:1927650/1‑71 (MQ=21) cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:4093562/69‑1 (MQ=25) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:1376215/66‑1 (MQ=25) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc > 1:2051226/1‑71 (MQ=21) acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt < 1:2017200/43‑1 (MQ=255) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc > 1:3399533/1‑70 (MQ=25) acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt < 1:1254822/71‑1 (MQ=21) acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt < 1:1369746/71‑1 (MQ=21) acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt > 1:531396/1‑71 (MQ=21) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGa < 1:2246815/35‑1 (MQ=255) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg > 1:811992/1‑71 (MQ=21) atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg > 1:2973316/1‑70 (MQ=25) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAg < 1:120247/70‑1 (MQ=25) | AATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG > minE/204493‑204622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |