| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,107,580 | Δ1 bp | 8.6% | coding (518/1155 nt) | agaS → | tagatose‑6‑phosphate ketose/aldose isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,107,580 | 0 | C | . | 8.6% | 228.2 / 12.1 | 70 | coding (518/1155 nt) | agaS | tagatose‑6‑phosphate ketose/aldose isomerase |
| Reads supporting (aligned to +/- strand): ref base C (28/36); new base . (2/4); total (30/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01 | |||||||||||
CAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAAC > minE/2107515‑2107649 | cAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACcag > 1:2743016/1‑69 (MQ=255) aaCAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACcagcag < 1:3902207/71‑1 (MQ=255) aCAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCagcagc < 1:3885543/71‑1 (MQ=255) aCAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCagcagc < 1:1816684/71‑1 (MQ=255) cAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAgcagca > 1:2112192/1‑71 (MQ=255) cAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAgcagca > 1:352058/1‑71 (MQ=255) gATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTac < 1:866249/71‑1 (MQ=255) tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTacca > 1:1087611/1‑71 (MQ=255) tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGA‑CAGCAGCATTaccac < 1:3712311/71‑1 (MQ=255) aCGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTaccacc < 1:1757361/71‑1 (MQ=255) aCGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTaccacc < 1:1538669/71‑1 (MQ=255) gcgTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:4024413/71‑1 (MQ=255) gcgTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:1911042/71‑1 (MQ=255) gTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:3964107/69‑1 (MQ=255) tttGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCatga > 1:2889463/1‑70 (MQ=255) tGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATgg > 1:1358065/1‑71 (MQ=255) tGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGA‑CAgcagca > 1:3203737/1‑55 (MQ=255) ccTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTaccac > 1:542018/1‑60 (MQ=255) ccTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:3729958/63‑1 (MQ=255) tgctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:841718/61‑1 (MQ=255) tgctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGc > 1:2354246/1‑71 (MQ=255) gctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCt > 1:282291/1‑71 (MQ=255) gctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCt > 1:2029299/1‑71 (MQ=255) ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGAc > 1:2840326/1‑41 (MQ=255) ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCatga > 1:1582890/1‑61 (MQ=255) ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCt > 1:4061356/1‑70 (MQ=255) tGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:2080994/55‑1 (MQ=255) gCCCGCAGAAACGGACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCatga > 1:548341/1‑56 (MQ=255) gCCCGCAGAAACGCACGATCGCGGCTTTGCGATGA‑CAGCAGCATTACCACCATGATGGCCAGCTGCCTcgc > 1:793527/1‑71 (MQ=255) cccGCAGAAACGCACGATCGCGGCTTTGCGATGACCagcagc < 1:1027772/42‑1 (MQ=255) cGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:396581/51‑1 (MQ=255) gCAGAAACGCACGATCGCGGCTTTGCGATGA‑CAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGtt < 1:2611147/71‑1 (MQ=255) cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTacca > 1:3614039/1‑45 (MQ=255) cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTacca > 1:3749513/1‑45 (MQ=255) cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGtt < 1:2723828/71‑1 (MQ=255) cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGtt > 1:3776482/1‑71 (MQ=255) aGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGttt > 1:2779330/1‑71 (MQ=255) gAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCatg < 1:4098732/48‑1 (MQ=255) aaaCGCACGATCGCGGCTTTGCGATGA‑CAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCg < 1:3206515/71‑1 (MQ=255) aaCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGc < 1:3211708/51‑1 (MQ=255) aaCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCg > 1:1086344/1‑71 (MQ=255) aaCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCg < 1:1739897/71‑1 (MQ=255) aCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCatga > 1:829153/1‑46 (MQ=255) aCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGc > 1:298750/1‑54 (MQ=255) aCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGc > 1:3830217/1‑54 (MQ=255) aCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGtt < 1:224720/66‑1 (MQ=255) aCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGtt > 1:1176014/1‑66 (MQ=255) aCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGtt > 1:3389671/1‑66 (MQ=255) aCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGc < 1:1983767/71‑1 (MQ=255) cGCACGATCGCGGCTTTGCGATGACCAGCAGCAtt < 1:812596/35‑1 (MQ=255) cACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:2484184/41‑1 (MQ=255) aCGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCt < 1:2867371/71‑1 (MQ=255) aCGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCt < 1:1272960/71‑1 (MQ=255) aCGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCt < 1:1276449/71‑1 (MQ=255) cGATCGCGGCTTTGCGATGA‑CAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTga < 1:2511651/71‑1 (MQ=255) gATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCt > 1:2020251/1‑69 (MQ=255) gATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTga < 1:867055/71‑1 (MQ=255) aTCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:3308578/37‑1 (MQ=255) aTCGCGGCTTTGCGATGACCAGCAGCATTACCACCat < 1:2749906/37‑1 (MQ=255) aTCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTgag < 1:2539405/71‑1 (MQ=255) aTCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTgag < 1:2095942/71‑1 (MQ=255) tCGCGGCTTTGCGATGACCAGCAGCATTACCACCatga > 1:2917036/1‑38 (MQ=255) tCGCGGCTTTGCGATGACCAGCAGCATTACCACCatga > 1:1586726/1‑38 (MQ=255) tCGCGGCTTTGCGATGACCAGCAGCATTACCACCatga > 1:767564/1‑38 (MQ=255) ggCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGAt < 1:2382148/71‑1 (MQ=255) gCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATc < 1:649323/71‑1 (MQ=255) tGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCaa < 1:3159680/69‑1 (MQ=255) gATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTgag < 1:2406790/58‑1 (MQ=255) tGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCaa < 1:3489366/71‑1 (MQ=255) tGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCaa < 1:3484750/71‑1 (MQ=255) aCCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAAc > 1:2245116/1‑71 (MQ=255) | CAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAAC > minE/2107515‑2107649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |