Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
JC | minE | 2,018,222 | Δ3 bp | 10.0% | coding (108‑110/1164 nt) | yqhD → | alcohol dehydrogenase, NAD(P)‑dependent |
New junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | minE | 2018225 = | 43 (0.720) | 4 (0.080) | 3/84 | NT | 10.0% | coding (111/1164 nt) | yqhD | alcohol dehydrogenase, NAD(P)‑dependent |
? | minE | = 2018221 | 35 (0.680) | coding (107/1164 nt) | yqhD | alcohol dehydrogenase, NAD(P)‑dependent |
AATTCCAGCACGTCCATGCCTTTCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < minE/2018299‑2018225 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccgccgTAGGTAATCAATACGCGAGCATCGTGAGGAATTTGTTCGCGTAAACCAGCGATTGCGCCTTTACCA < minE/2018221‑2018156 AATTCCAGCACGTCCATGCCTTTCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCC > 1:1737215/1‑71 AATTCCAGCACGTCCATGCCTTTCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCC > 1:929961/1‑71 TCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGC > 1:1988300/1‑51 TCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGC < 1:870453/43‑1 GAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCGTAGGTAATCAA > 1:615561/1‑52 ATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCGTAGGTAATCAATACGC < 1:3412024/50‑1 ATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCGTAGGTAATCAATACGC < 1:64856/50‑1 TTTTTTTCACGCCGCCGCCGTAGGTAATCAATACGCGAGCATCGTGAGGAATTTG < 1:1465268/55‑1 CGCCGTAGGTAATCAATACGCGAGCATCGTGAGGAATTTGTTCGCGTAAACCAGCGATTGCGCCTTTACCA < 1:3917629/71‑1 CCGTAGGTAATCAATACGCGAGCATCGTGAGGAATTTGTTCGCGTAAACCAGCGATTGCGCCTTTAC < 1:2130389/67‑1 AATTCCAGCACGTCCATGCCTTTCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < minE/2018299‑2018225 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccgccgTAGGTAATCAATACGCGAGCATCGTGAGGAATTTGTTCGCGTAAACCAGCGATTGCGCCTTTACCA < minE/2018221‑2018156 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |