Predicted mutation
evidence seq id position mutation freq annotation gene description
JC minE 2,018,222 Δ3 bp 10.0% coding (108‑110/1164 nt) yqhD → alcohol dehydrogenase, NAD(P)‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE 2018225 =43 (0.720)4 (0.080) 3/84 NT 10.0% coding (111/1164 nt) yqhD alcohol dehydrogenase, NAD(P)‑dependent
?minE = 2018221 35 (0.680)coding (107/1164 nt) yqhD alcohol dehydrogenase, NAD(P)‑dependent

AATTCCAGCACGTCCATGCCTTTCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  minE/2018299‑2018225
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccgccgTAGGTAATCAATACGCGAGCATCGTGAGGAATTTGTTCGCGTAAACCAGCGATTGCGCCTTTACCA  <  minE/2018221‑2018156
                                                                                                                                             
AATTCCAGCACGTCCATGCCTTTCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCC                                                                        >  1:1737215/1‑71
AATTCCAGCACGTCCATGCCTTTCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCC                                                                        >  1:929961/1‑71
                      TCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGC                                                                      >  1:1988300/1‑51
                              TCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGC                                                                      <  1:870453/43‑1
                                  GAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCGTAGGTAATCAA                                                         >  1:615561/1‑52
                                         ATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCGTAGGTAATCAATACGC                                                    <  1:3412024/50‑1
                                         ATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCGTAGGTAATCAATACGC                                                    <  1:64856/50‑1
                                                       TTTTTTTCACGCCGCCGCCGTAGGTAATCAATACGCGAGCATCGTGAGGAATTTG                                 <  1:1465268/55‑1
                                                                      CGCCGTAGGTAATCAATACGCGAGCATCGTGAGGAATTTGTTCGCGTAAACCAGCGATTGCGCCTTTACCA  <  1:3917629/71‑1
                                                                        CCGTAGGTAATCAATACGCGAGCATCGTGAGGAATTTGTTCGCGTAAACCAGCGATTGCGCCTTTAC    <  1:2130389/67‑1
                                                                                                                                             
AATTCCAGCACGTCCATGCCTTTCAGGGCATCCAGAACTTGATCGAGAACGCCGGTTTTTTTCACGCTGCCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  minE/2018299‑2018225
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccgccgTAGGTAATCAATACGCGAGCATCGTGAGGAATTTGTTCGCGTAAACCAGCGATTGCGCCTTTACCA  <  minE/2018221‑2018156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.