New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE 2548248 =93 (1.550)3 (0.060) 3/86 NT 3.8% coding (457/858 nt) htrL hypothetical protein
?minE 2548278 = 71 (1.350)coding (487/858 nt) htrL hypothetical protein
Rejected: Frequency below/above cutoff threshold.

TTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  minE/2548323‑2548248
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atttggGATTTCCCATTTGATGAAAGTAAGATGCATCTTTTCACTATAAAAAAAGGGCTTACTGTCACATCTC  >  minE/2548278‑2548344
                                                                                                                                               
TTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACA                                                                          >  1:3689130/1‑71
TTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACA                                                                          >  1:353650/1‑71
 TTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACA                                                                          <  1:1712979/70‑1
 TTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACA                                                                          <  1:3185502/70‑1
 TTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACA                                                                          <  1:2146456/70‑1
  TTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACAT                                                                         <  1:891277/70‑1
                        TACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTG                                                                      <  1:3457324/51‑1
                         ACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATT                                                                        >  1:2632214/1‑48
                            TTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGG                                                                     >  1:3218707/1‑48
                             TCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGG                                                                     >  1:1959799/1‑47
                             TCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACA                                                                          >  1:219210/1‑42
                                     TGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTT                                                                       >  1:2497903/1‑37
                                     TGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTT                                                                       >  1:2886922/1‑37
                                     TGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTT                                                                       >  1:1940261/1‑37
                                              CCAAATTTTCAATCCTCGAGTTACATTTGGGATTTCCCATTTGATGA                                                    <  1:3007095/47‑1
                                                       CAATCCTCGAGTTACATTTGGGATTTCCCATTTGATGAAAGTAAGATGCATCTTTTCACTATAAAAAAAGG                   >  1:506297/1‑71
                                                            CTCGAGTTACATTTGGGATTTCCCATTTGATGAAAGTAAGATGCATCTTTTCACTATAAAAAAAGGGCTTA              <  1:3375979/71‑1
                                                                      ATTTGTGATTTCCCATTTGATGAAAGTAAGATGCATCTTTTCACTATAAAAAAAGGGCTTA              <  1:288293/61‑1
                                                                         TGGGATTTCCCATTTGATGAAAGTAAGATGCATCTTTTCACTATAAAAAAAGGGCTTACTGTCACATCTC  >  1:233239/1‑70
                                                                           GGATTTCCCATTTGATGAAAGTAAGATGCATCTTTTCACTATAAAAA                       <  1:1988365/47‑1
                                                                           GGATTTCCCATTTGATGAAAGTAAGATGCATCTTTTCACTATAAAAA                       <  1:1650507/47‑1
                                                                                                                                               
TTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  minE/2548323‑2548248
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atttggGATTTCCCATTTGATGAAAGTAAGATGCATCTTTTCACTATAAAAAAAGGGCTTACTGTCACATCTC  >  minE/2548278‑2548344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.