| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,154 | C→T | 15.2% | D606D (GAC→GAT) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,154 | 0 | C | T | 15.2% | 152.8 / 16.5 | 59 | D606D (GAC→GAT) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base C (24/26); new base T (5/4); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTG > minE/2091‑2223 | gATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAAc < 1:3592978/70‑1 (MQ=255)gATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAAc < 1:2485792/70‑1 (MQ=255)gATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAAc < 1:3832260/70‑1 (MQ=255)gATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACg > 1:1953850/1‑71 (MQ=255) tGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGtt > 1:3787763/1‑71 (MQ=255) ggATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTg > 1:2091643/1‑71 (MQ=255) gATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTgg > 1:823320/1‑71 (MQ=255) aTTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTggg > 1:3871754/1‑71 (MQ=255) ttACTAACATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTggc > 1:1169609/1‑69 (MQ=255) actacCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGc < 1:3518061/70‑1 (MQ=255) ctacCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGtt > 1:770098/1‑64 (MQ=255) cATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGAtt < 1:3508382/71‑1 (MQ=255) aTCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTa < 1:3560165/71‑1 (MQ=255) tCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTAc > 1:3164118/1‑71 (MQ=255) tCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTAc > 1:157310/1‑71 (MQ=255) aGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAAc > 1:3645946/1‑53 (MQ=255) aGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTgg > 1:187416/1‑58 (MQ=255) ttGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGt < 1:1375647/71‑1 (MQ=255) ttGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGt < 1:1786112/71‑1 (MQ=255) ttGCGTTATGCGGCGGAAAAATCGCGGAGTAAATTCCTCTATGATACCAACGtt < 1:310234/54‑1 (MQ=255) gCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTg > 1:645579/1‑53 (MQ=255) gCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGtt < 1:370552/70‑1 (MQ=255) cGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTa < 1:1074582/70‑1 (MQ=255) cGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTa < 1:3158151/70‑1 (MQ=255) cGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTa < 1:718565/70‑1 (MQ=255) cGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTa < 1:4107384/70‑1 (MQ=255) cGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTa < 1:2217498/70‑1 (MQ=255) gTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTg < 1:1430542/57‑1 (MQ=255) aTGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGt > 1:1422376/1‑46 (MQ=255) aTGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAtt > 1:2782438/1‑68 (MQ=255) cggcggAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAAc < 1:3395948/71‑1 (MQ=255) gAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTggg < 1:2741405/42‑1 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTg > 1:118466/1‑44 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAt > 1:3406229/1‑57 (MQ=255) aTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGAt > 1:2389549/1‑44 (MQ=255) aTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTAcc > 1:1517953/1‑48 (MQ=255) aTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTg < 1:1808120/56‑1 (MQ=255) aTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATc > 1:1937706/1‑71 (MQ=255) cgcgGCGTAAATTCCTCTATGACACCAACGTTGAGGCTGGATTa < 1:1292493/44‑1 (MQ=255) gcggcgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTg < 1:101289/70‑1 (MQ=255) gcggcgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTg < 1:1404568/70‑1 (MQ=255) gcggcgTAAATTCCTATATGATACCAACGTTGGGGCTGGATTACCGGTTATTgaga < 1:1323130/56‑1 (MQ=255) ggcgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCt > 1:1762078/1‑70 (MQ=255) cgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaa < 1:2565751/61‑1 (MQ=255) cgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCa < 1:3726896/70‑1 (MQ=255) aaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaaa > 1:1944064/1‑58 (MQ=255) aaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCt > 1:1438985/1‑61 (MQ=255) aaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAAt > 1:2350764/1‑68 (MQ=255) aaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATg > 1:1719154/1‑69 (MQ=255) aaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCa < 1:587608/71‑1 (MQ=255) ttCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaa < 1:1888937/55‑1 (MQ=255) tCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCaa > 1:1533789/1‑64 (MQ=255) tCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGt > 1:2874432/1‑71 (MQ=255) tCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGt > 1:2295963/1‑71 (MQ=255) ctctATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCa > 1:745267/1‑61 (MQ=255) tATGACACCAACGTTGGGGCTGGATTACCGGTTATTgag < 1:1952936/39‑1 (MQ=255) tATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAAc > 1:1039317/1‑42 (MQ=255) tGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGtgatg < 1:624053/68‑1 (MQ=255) tGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGtgatg < 1:1259292/68‑1 (MQ=255) acacCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAAtt > 1:3912392/1‑70 (MQ=255) acacCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAAtt < 1:1740662/70‑1 (MQ=255) acacCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAAtt > 1:1062668/1‑70 (MQ=255) acacCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAAtt < 1:2580172/70‑1 (MQ=255) acacCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATtg < 1:1738052/71‑1 (MQ=255) cacCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGtgatg < 1:512690/65‑1 (MQ=255) | GATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTG > minE/2091‑2223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |