| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 183,899 | G→A | 45.3% | L194L (CTG→CTA) | rnhB → | ribonuclease HII, degrades RNA of DNA‑RNA hybrids |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 183,899 | 0 | G | A | 45.3% | 18.4 / 82.8 | 64 | L194L (CTG→CTA) | rnhB | ribonuclease HII, degrades RNA of DNA‑RNA hybrids |
| Reads supporting (aligned to +/- strand): ref base G (19/16); new base A (19/10); total (38/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
ACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTT > minE/183836‑183967 | aCTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTg > 1:2108922/1‑71 (MQ=255)aCTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTg > 1:1971166/1‑71 (MQ=255) cTGGCTGAACACGGCGCGCCCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGc < 1:2367234/71‑1 (MQ=255) gCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTc > 1:4026787/1‑71 (MQ=255) gCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGa > 1:3905470/1‑63 (MQ=255) aaCACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGa < 1:2716480/71‑1 (MQ=255) aaCACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGa < 1:2523056/71‑1 (MQ=255) aaCACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCt < 1:2697777/69‑1 (MQ=255) aaCACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGAGTcc > 1:3570250/1‑68 (MQ=255) cacGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGAtt > 1:1736530/1‑71 (MQ=255) cacGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGa > 1:3137124/1‑69 (MQ=255) cacGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGAtt > 1:410574/1‑71 (MQ=255) cacGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGAtt > 1:1114019/1‑71 (MQ=255) gcgACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTtgtg > 1:2557607/1‑71 (MQ=255) ccGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCg < 1:1689227/70‑1 (MQ=255) cGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGc < 1:1538382/51‑1 (MQ=255) gAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGc > 1:3315717/1‑50 (MQ=255) acacCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGAtt < 1:1238201/71‑1 (MQ=255) acCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTc < 1:173403/49‑1 (MQ=255) ccATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAg > 1:2847782/1‑71 (MQ=255) aTCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTa < 1:1331563/71‑1 (MQ=255) cGGCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCg < 1:274066/59‑1 (MQ=255) ggCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAAc > 1:452787/1‑71 (MQ=255) ggCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACATGCGTCCTGATTCTtg > 1:3848578/1‑53 (MQ=255) ggCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACATGCGTCCTGATTCTTGTGTCGAGATTAAGTAAAc > 1:1624471/1‑71 (MQ=255) ggCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACATGCGTCCTGATTCTTGTGTCGAGATTAAGTAAAc > 1:439155/1‑71 (MQ=255) ggCGCAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTCTGTCGAGAt < 1:4076781/62‑1 (MQ=255) gcAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCg > 1:3994332/1‑70 (MQ=255) cAGCTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGa < 1:304314/71‑1 (MQ=255) gCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTc > 1:160583/1‑37 (MQ=255) gCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTc > 1:442989/1‑51 (MQ=255) gCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAAt < 1:232336/71‑1 (MQ=255) cTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATc < 1:4013391/71‑1 (MQ=255) cTTTGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCt < 1:2745678/38‑1 (MQ=255) ttGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCt > 1:2597466/1‑70 (MQ=255) ttGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCt > 1:2558302/1‑70 (MQ=255) ttGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCt > 1:3282564/1‑70 (MQ=255) ttGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCt > 1:2996187/1‑70 (MQ=255) tGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTg > 1:763912/1‑70 (MQ=255) tGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTg > 1:2953477/1‑70 (MQ=255) tGGGCCTGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTg < 1:2547031/70‑1 (MQ=255) ggCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTaaa > 1:2893262/1‑58 (MQ=255) ggCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAg < 1:1014229/71‑1 (MQ=255) cTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCga > 1:1512263/1‑44 (MQ=255) tGTCAAACGCGCACTGGGACTTGCGTCCTGATTCtt > 1:2207368/1‑36 (MQ=255) tGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCgg < 1:2748144/58‑1 (MQ=255) tGTCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTaa < 1:1994631/49‑1 (MQ=255) tCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCga < 1:3602035/41‑1 (MQ=255) tCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTaaa < 1:2155456/52‑1 (MQ=255) tCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGTAATCTGAAGATg < 1:325561/68‑1 (MQ=255) tCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCt > 1:2902220/1‑71 (MQ=255) tCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCgaga > 1:3110429/1‑43 (MQ=255) tCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTa < 1:3010122/46‑1 (MQ=255) tCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTa > 1:2925016/1‑50 (MQ=255) tCAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTg < 1:2189479/62‑1 (MQ=255) cAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTaaa > 1:2244278/1‑51 (MQ=255) cAAACGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAAc > 1:3454213/1‑52 (MQ=255) aaaCGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGAt > 1:3936647/1‑65 (MQ=255) aaaCGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGAt > 1:1077376/1‑65 (MQ=255) aaaCGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTaaa > 1:2528988/1‑50 (MQ=255) aaaCGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCt > 1:1269547/1‑69 (MQ=255) aaaCGCGCACTAGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTg > 1:2653398/1‑70 (MQ=255) aCGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAAc < 1:3514454/71‑1 (MQ=255) cTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGttt < 1:2063254/71‑1 (MQ=255) | ACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTT > minE/183836‑183967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |