| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,811 | A→C | 7.8% | V354V (GTT→GTG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,811 | 0 | A | C | 7.8% | 242.7 / 14.3 | 90 | V354V (GTT→GTG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (42/41); new base C (2/5); total (44/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACG > minE/311741‑311881 | cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:918311/71‑1 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc > 1:3695114/1‑71 (MQ=255) gCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAcc < 1:171374/71‑1 (MQ=255) acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc < 1:613047/69‑1 (MQ=2) aTACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:2620909/71‑1 (MQ=255) tACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:1574379/1‑65 (MQ=255) tACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:3207598/1‑65 (MQ=255) accacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGaa > 1:3657808/1‑71 (MQ=2) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTGTGCAGGAACAGATACATAACCa > 1:1858654/1‑66 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:1292228/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:3087128/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:22376/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:3295309/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:75649/1‑70 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:2716727/71‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:2975405/71‑1 (MQ=255) ccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAaca > 1:2776294/1‑69 (MQ=255) ccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg < 1:3403257/71‑1 (MQ=255) gTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAgg < 1:2146543/71‑1 (MQ=255) aCGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAg > 1:1099032/1‑59 (MQ=255) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCa > 1:130830/1‑57 (MQ=255) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:3088395/71‑1 (MQ=255) cAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:935959/1‑51 (MQ=255) cAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:4087146/1‑51 (MQ=255) cAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:1229588/1‑51 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:998136/53‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAgg < 1:2365597/62‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:2181292/65‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:1804984/65‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCt < 1:1241613/71‑1 (MQ=255) ggTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGc > 1:546188/1‑69 (MQ=255) gTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAaca > 1:3097006/1‑55 (MQ=255) gTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAaca > 1:229335/1‑55 (MQ=255) tCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTc > 1:3433327/1‑70 (MQ=255) ggAATCAACGTCTCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGAc > 1:3416269/1‑71 (MQ=255) ggAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa < 1:352620/70‑1 (MQ=255) ggAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa < 1:3150179/70‑1 (MQ=255) ggAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa < 1:2647656/70‑1 (MQ=255) aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa < 1:3400413/48‑1 (MQ=255) aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:2835448/1‑54 (MQ=255) aTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAgg < 1:2680220/54‑1 (MQ=255) aTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:4032425/57‑1 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:289060/38‑1 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAg > 1:4103127/1‑43 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:711709/56‑1 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:784997/56‑1 (MQ=255) aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:524253/1‑50 (MQ=255) aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat > 1:3307076/1‑56 (MQ=255) aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATc > 1:2900565/1‑57 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCa > 1:3309955/1‑36 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:3841210/50‑1 (MQ=255) cgcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAgg > 1:2766538/1‑46 (MQ=255) cgcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:706391/1‑71 (MQ=255) cgcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:3432227/1‑71 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGga > 1:622806/1‑46 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:3644790/1‑70 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc > 1:3917369/1‑71 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc > 1:2901924/1‑71 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc < 1:2472535/71‑1 (MQ=255) cgGAAGTTCTGCAGGAACAGATACATAACCCGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc < 1:3786173/71‑1 (MQ=255) ggAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACt > 1:2842000/1‑71 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCt < 1:2356451/49‑1 (MQ=255) atattCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTc < 1:1257570/68‑1 (MQ=16) atattCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTc < 1:4132534/68‑1 (MQ=16) attCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGt > 1:3806065/2‑71 (MQ=18) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:3151805/71‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:3051962/71‑1 (MQ=255) cTGCAGGAACAGAAACATAACCAGGAACACGAGGAAGATCGCTTCGACCAGCGTTTTAACCACt > 1:2082536/1‑64 (MQ=255) caagAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGaa < 1:2376885/65‑1 (MQ=21) cAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGaa > 1:3926964/1‑68 (MQ=255) aagAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGaa < 1:3641581/65‑1 (MQ=21) aGGAACAGATACATAACCAGGAACACGAGgatgat > 1:2525127/1‑35 (MQ=255) ggAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATagag < 1:3727808/71‑1 (MQ=255) aCAGATACATAACCAGGAACACGAGGATGATCGCt < 1:709071/35‑1 (MQ=255) aCAGATACATAACCAGGAACACGAGGATGATCGCt < 1:2457115/35‑1 (MQ=255) cAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCG‑TTTAACCACTTCGTGAATAGAGAtttt < 1:282269/71‑1 (MQ=255) aGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:1906737/71‑1 (MQ=255) aGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAttt > 1:3224490/1‑70 (MQ=255) gATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:3809363/1‑45 (MQ=255) gATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTc < 1:1770351/71‑1 (MQ=255) aTACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATa > 1:3316468/1‑61 (MQ=255) aTACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:360248/69‑1 (MQ=255) tACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc < 1:3823302/71‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTc < 1:1019695/52‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTc < 1:2960730/52‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTc < 1:2870579/52‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGt < 1:3108121/54‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGt < 1:683765/54‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:2335233/55‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt > 1:2972066/1‑67 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc > 1:4121586/1‑70 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:2657994/53‑1 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:66945/1‑71 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:442460/1‑71 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:906935/1‑71 (MQ=255) tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAc > 1:2195688/1‑71 (MQ=255) tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAc > 1:2112705/1‑71 (MQ=255) aaCCAGGAACACGTGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg > 1:1061898/1‑71 (MQ=255) | CGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACG > minE/311741‑311881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |