| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,493 | T→A | 13.0% | Y68F (TAT→TTT) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,493 | 0 | T | A | 13.0% | 203.6 / 21.5 | 77 | Y68F (TAT→TTT) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base T (31/36); new base A (5/5); total (36/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
TAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAC > minE/2919425‑2919562 | tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGATATAAGTAACGCGAGCACCCTGGTCatat > 1:1293599/1‑71 (MQ=255)tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCatat > 1:1165112/1‑71 (MQ=255)tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCata > 1:150592/1‑70 (MQ=255) aaTTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat < 1:2411471/70‑1 (MQ=255) aaTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATg > 1:968957/1‑71 (MQ=255) ctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCgg > 1:2365575/1‑66 (MQ=255) ctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACt > 1:1606068/1‑71 (MQ=255) tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACtt > 1:2953914/1‑71 (MQ=255) ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc > 1:1942956/1‑71 (MQ=255) tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc > 1:4092910/1‑70 (MQ=255) tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:1186988/70‑1 (MQ=255) tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:1920390/70‑1 (MQ=255) cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAagag > 1:767605/1‑71 (MQ=255) cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAagag > 1:3267248/1‑71 (MQ=255) cAATCTGGCTGCCGCTCGGCCCTAAATAGGTACCGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAaga > 1:891979/1‑70 (MQ=255) aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCat > 1:2647817/1‑49 (MQ=255) aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa > 1:95545/1‑66 (MQ=255) aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa < 1:48623/71‑1 (MQ=255) aTCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGa < 1:3847780/64‑1 (MQ=255) tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc < 1:3973899/55‑1 (MQ=255) cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGc > 1:1688406/1‑51 (MQ=255) gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCAt < 1:318720/67‑1 (MQ=255) gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc < 1:356214/68‑1 (MQ=255) gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:3869213/71‑1 (MQ=255) gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:1261065/71‑1 (MQ=255) tGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa > 1:4116215/1‑71 (MQ=255) gCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc > 1:581768/1‑71 (MQ=255) ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCata > 1:1537967/1‑41 (MQ=255) ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCata > 1:2400577/1‑41 (MQ=255) ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:1212922/70‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATg > 1:3879582/1‑42 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAATAGCATCGGGTAcga < 1:1028387/71‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa > 1:1882358/1‑61 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:3570112/71‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:683439/71‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:504669/71‑1 (MQ=255) tGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc > 1:724116/1‑70 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:2970995/69‑1 (MQ=255) gCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:1637446/59‑1 (MQ=255) cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:3624535/1‑67 (MQ=255) gagaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtt > 1:1292363/1‑71 (MQ=255) gagaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtt > 1:3634090/1‑71 (MQ=255) agaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:728890/71‑1 (MQ=255) agaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:3711207/71‑1 (MQ=255) gaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt > 1:2520862/1‑71 (MQ=255) aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCtttt > 1:3865966/2‑71 (MQ=255) aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:964656/61‑1 (MQ=255) tAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:1678567/57‑1 (MQ=255) aaGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGc > 1:2792783/1‑45 (MQ=255) aaGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:2968530/1‑61 (MQ=255) gTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGaa < 1:676591/71‑1 (MQ=255) gTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGaa < 1:2659332/71‑1 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:1808338/1‑59 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:2809018/71‑1 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:3770557/71‑1 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:2317538/71‑1 (MQ=255) tAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGGCTTTTTCGAAg < 1:1855672/71‑1 (MQ=255) aaCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCAt > 1:3342155/1‑43 (MQ=255) aaCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCg > 1:2955093/1‑67 (MQ=255) aaCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAg < 1:100459/70‑1 (MQ=255) aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:3656974/68‑1 (MQ=255) aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:403243/68‑1 (MQ=255) cgcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATg < 1:2924942/71‑1 (MQ=255) cgcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATg < 1:1830995/71‑1 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAt > 1:2283473/1‑68 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa > 1:2698401/1‑70 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAg > 1:2666823/1‑71 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTAGAAGATg > 1:688405/1‑69 (MQ=255) gCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:555993/1‑50 (MQ=255) cgcccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGcgcg < 1:1884764/69‑1 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:1242539/39‑1 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:30519/39‑1 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt < 1:2121779/53‑1 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATg < 1:2486895/62‑1 (MQ=255) ccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATg < 1:1777323/71‑1 (MQ=255) cTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:3336708/35‑1 (MQ=255) cTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGa > 1:2282761/1‑59 (MQ=255) cTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATg < 1:329397/61‑1 (MQ=255) tGGTCATATGCGGCAACTTCGAAAGAGCATCGCGTACGAGTCGAGTCt > 1:2178340/1‑48 (MQ=255) tGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtt > 1:1681307/1‑71 (MQ=255) ggTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAcgagt > 1:3059741/1‑40 (MQ=255) ggTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGttt > 1:3268068/1‑71 (MQ=255) gTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtt < 1:2059077/69‑1 (MQ=255) tCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGttttt < 1:2276910/71‑1 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:1994963/51‑1 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGa > 1:143603/1‑62 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc > 1:2623331/1‑71 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc < 1:3429740/71‑1 (MQ=255) tatGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:956578/36‑1 (MQ=255) tatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGttttt < 1:3025664/68‑1 (MQ=255) | TAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAC > minE/2919425‑2919562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |