| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,516 | T→A | 14.6% | R60R (CGA→CGT) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,516 | 0 | T | A | 14.6% | 249.7 / 32.2 | 96 | R60R (CGA→CGT) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base T (28/54); new base A (7/7); total (35/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTT > minE/2919450‑2919584 | gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCAt < 1:318720/67‑1 (MQ=255)gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc < 1:356214/68‑1 (MQ=255)gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:3869213/71‑1 (MQ=255)gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:1261065/71‑1 (MQ=255) tGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa > 1:4116215/1‑71 (MQ=255) gCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc > 1:581768/1‑71 (MQ=255) ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:1212922/70‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAATAGCATCGGGTAcga < 1:1028387/71‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:3570112/71‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:504669/71‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:683439/71‑1 (MQ=255) tGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc > 1:724116/1‑70 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:2970995/69‑1 (MQ=255) gCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:1637446/59‑1 (MQ=255) cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:3624535/1‑67 (MQ=255) gagaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtt > 1:1292363/1‑71 (MQ=255) gagaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtt > 1:3634090/1‑71 (MQ=255) agaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:728890/71‑1 (MQ=255) agaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:3711207/71‑1 (MQ=255) gaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt > 1:2520862/1‑71 (MQ=255) aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCtttt > 1:3865966/2‑71 (MQ=255) aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:964656/61‑1 (MQ=255) tAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:1678567/57‑1 (MQ=255) aaGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:2968530/1‑61 (MQ=255) gTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGaa < 1:676591/71‑1 (MQ=255) gTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGaa < 1:2659332/71‑1 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:1808338/1‑59 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:3770557/71‑1 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:2809018/71‑1 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:2317538/71‑1 (MQ=255) tAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGGCTTTTTCGAAg < 1:1855672/71‑1 (MQ=255) aaCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCAt > 1:3342155/1‑43 (MQ=255) aaCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCg > 1:2955093/1‑67 (MQ=255) aaCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAg < 1:100459/70‑1 (MQ=255) aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:403243/68‑1 (MQ=255) aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:3656974/68‑1 (MQ=255) cgcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATg < 1:1830995/71‑1 (MQ=255) cgcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATg < 1:2924942/71‑1 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAt > 1:2283473/1‑68 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa > 1:2698401/1‑70 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAg > 1:2666823/1‑71 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTAGAAGATg > 1:688405/1‑69 (MQ=255) gCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:555993/1‑50 (MQ=255) cgcccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGcgcg < 1:1884764/69‑1 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:1242539/39‑1 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:30519/39‑1 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt < 1:2121779/53‑1 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATg < 1:2486895/62‑1 (MQ=255) ccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATg < 1:1777323/71‑1 (MQ=255) cTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:3336708/35‑1 (MQ=255) cTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGa > 1:2282761/1‑59 (MQ=255) cTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATg < 1:329397/61‑1 (MQ=255) tGGTCATATGCGGCAACTTCGAAAGAGCATCGCGTACGAGTCGAGTCt > 1:2178340/1‑48 (MQ=255) tGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtt > 1:1681307/1‑71 (MQ=255) ggTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAcgagt > 1:3059741/1‑40 (MQ=255) ggTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGttt > 1:3268068/1‑71 (MQ=255) gTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtt < 1:2059077/69‑1 (MQ=255) tCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGttttt < 1:2276910/71‑1 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:1994963/51‑1 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGa > 1:143603/1‑62 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc < 1:3429740/71‑1 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc > 1:2623331/1‑71 (MQ=255) tatGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:956578/36‑1 (MQ=255) tatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGttttt < 1:3025664/68‑1 (MQ=255) tGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:3419397/1‑36 (MQ=255) tGCGGCAACTTCGAAAGAGCATCGGGTACgagtcgagtc > 1:3520141/1‑39 (MQ=255) tGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa > 1:1185471/1‑48 (MQ=255) gCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAccgg > 1:3511448/1‑68 (MQ=255) cGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtt < 1:4107318/61‑1 (MQ=255) ggCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAAGTTTTTACCAGt < 1:3453768/69‑1 (MQ=255) gCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtttt < 1:892166/61‑1 (MQ=255) aaCTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:3286013/36‑1 (MQ=255) aaCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTc > 1:1690998/1‑39 (MQ=255) aaCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa > 1:815364/1‑42 (MQ=255) cTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTg < 1:457845/71‑1 (MQ=255) cTTCGAAAGAGCAACGGGTACGATTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttc < 1:2919292/71‑2 (MQ=255) ttCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAg < 1:2729500/66‑1 (MQ=255) ttCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTg > 1:3770343/1‑70 (MQ=255) tCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAcc > 1:1375800/1‑59 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttctg > 1:1068263/1‑67 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttctg > 1:392637/1‑67 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttct < 1:1149638/69‑3 (MQ=255) gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAg < 1:2062205/37‑1 (MQ=255) gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAg < 1:130122/37‑1 (MQ=255) gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGctt < 1:952633/71‑1 (MQ=255) gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGct < 1:1519431/70‑1 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttct < 1:467653/71‑1 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttct < 1:3823534/71‑1 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttc < 1:3578458/70‑1 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGACTTTGGcttct > 1:3374360/1‑71 (MQ=255) agagCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGt < 1:1031158/47‑1 (MQ=255) agagCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAgc < 1:905995/61‑2 (MQ=255) gagCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTa > 1:131459/1‑51 (MQ=255) gagCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc < 1:330008/52‑1 (MQ=255) gagCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGttt < 1:100857/62‑1 (MQ=255) gCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGttt < 1:2674380/60‑1 (MQ=255) gCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTCTACCAGTGAGTTTGGcttcttctt < 1:1939964/71‑1 (MQ=255) cATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCatt < 1:3717555/71‑1 (MQ=255) tCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTa < 1:2780335/46‑1 (MQ=255) | GCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTT > minE/2919450‑2919584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |