| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,567 | G→A | 8.3% | L43L (CTC→CTT) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,567 | 0 | G | A | 8.3% | 226.2 / 7.2 | 72 | L43L (CTC→CTT) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base G (25/41); new base A (2/4); total (27/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTT > minE/2919503‑2919635 | cTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTg < 1:457845/71‑1 (MQ=255)cTTCGAAAGAGCAACGGGTACGATTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttc < 1:2919292/71‑2 (MQ=255) ttCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAg < 1:2729500/66‑1 (MQ=255) ttCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTg > 1:3770343/1‑70 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttctg > 1:1068263/1‑67 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttctg > 1:392637/1‑67 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttct < 1:1149638/69‑3 (MQ=255) gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGctt < 1:952633/71‑1 (MQ=255) gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGct < 1:1519431/70‑1 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttct < 1:467653/71‑1 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttct < 1:3823534/71‑1 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttc < 1:3578458/70‑1 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGACTTTGGcttct > 1:3374360/1‑71 (MQ=255) agagCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAgc < 1:905995/61‑2 (MQ=255) gagCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGttt < 1:100857/62‑1 (MQ=255) gCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGttt < 1:2674380/60‑1 (MQ=255) gCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTCTACCAGTGAGTTTGGcttcttctt < 1:1939964/71‑1 (MQ=255) cATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCatt < 1:3717555/71‑1 (MQ=255) cgagtcgagtCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCttt < 1:3155227/70‑1 (MQ=255) gagtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGt < 1:2125763/47‑1 (MQ=255) gagtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCtt < 1:3854496/68‑1 (MQ=255) gtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCtt < 1:1232633/71‑1 (MQ=255) gtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCt > 1:255071/1‑70 (MQ=255) tcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTa > 1:3780291/1‑71 (MQ=255) gagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATc < 1:501393/71‑1 (MQ=255) gagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATc < 1:4054159/71‑1 (MQ=255) agtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTg < 1:899249/38‑1 (MQ=255) gtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTCTGGCTTCTTCTTTAcc > 1:2137065/1‑57 (MQ=255) tcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAg < 1:814002/38‑1 (MQ=255) tcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTAt > 1:743782/1‑67 (MQ=255) tcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGc > 1:2023203/1‑71 (MQ=255) tttttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttcttctt > 1:246622/1‑50 (MQ=255) tttttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttctt > 1:579742/1‑47 (MQ=255) tttttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCtt > 1:1809560/1‑71 (MQ=255) tttttCGAAGATGAGCACGATGTTTTTACCAGTGAGttt < 1:1955299/39‑1 (MQ=255) tttttCGAAGATGAGAGCGATGTTTTTACCAGTGAGTTTGGc > 1:1444758/1‑42 (MQ=255) tttCGAAGATGAGCGCGATGTTTTTACCGGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTAt > 1:736587/1‑63 (MQ=255) tttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCttt < 1:3601709/70‑1 (MQ=255) tttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCt < 1:246912/68‑1 (MQ=255) tttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCt < 1:2086482/68‑1 (MQ=255) tCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCa < 1:1310845/70‑1 (MQ=255) cGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGc > 1:3073001/1‑71 (MQ=255) aaGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTc > 1:1383695/1‑54 (MQ=255) aaGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTc > 1:1046343/1‑54 (MQ=255) gATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTcg > 1:3248903/1‑71 (MQ=255) gtGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCgg < 1:179334/57‑1 (MQ=255) aTGAGCGCGATGTTTTTACCAGTGAGTTTGGcttcttctt < 1:826621/40‑1 (MQ=255) aTGAGCGCGATGTTTTTACCAGTGAGTTTGGcttcttc < 1:3021790/38‑1 (MQ=255) aTGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTcgc < 1:1640929/71‑1 (MQ=255) aGCGCGATGTTTTTACCAGTGAGTATGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGc > 1:1765994/1‑71 (MQ=255) gcgcGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCt < 1:976836/71‑1 (MQ=255) gcgcGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCt < 1:3638689/71‑1 (MQ=255) cgcgATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTa < 1:3166401/71‑1 (MQ=255) gcgATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTa > 1:343917/1‑36 (MQ=255) gcgATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTa > 1:100231/1‑36 (MQ=255) aTGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTg < 1:95183/71‑1 (MQ=255) aTGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTg < 1:1849651/71‑1 (MQ=255) gTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTaa > 1:3958553/1‑66 (MQ=255) ttttACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcag > 1:1348143/1‑70 (MQ=255) ttttACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcag > 1:498776/1‑70 (MQ=255) tttACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCtt > 1:1958947/1‑54 (MQ=255) tttACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCtt > 1:594043/1‑54 (MQ=255) tttACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcag > 1:3199911/1‑69 (MQ=255) aGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagca < 1:2387988/65‑1 (MQ=255) aGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagca < 1:2509406/65‑1 (MQ=255) aGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagca < 1:1825216/65‑1 (MQ=255) aGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagca < 1:2736414/65‑1 (MQ=255) aGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagca < 1:1192689/65‑1 (MQ=255) gTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGtt < 1:2475526/71‑1 (MQ=255) gTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGtt < 1:1268207/71‑1 (MQ=255) gTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGtc < 1:1120670/71‑2 (MQ=255) tGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCgg < 1:3791958/51‑1 (MQ=255) tGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCt < 1:280414/53‑1 (MQ=255) gAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAg > 1:1109434/1‑42 (MQ=255) | CTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTT > minE/2919503‑2919635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |