| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,936,957 | C→T | 15.4% | A213T (GCC→ACC) | yjgB ← | predicted alcohol dehydrogenase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,936,957 | 0 | C | T | 15.4% | 203.2 / 25.2 | 78 | A213T (GCC→ACC) | yjgB | predicted alcohol dehydrogenase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (33/33); new base T (9/3); total (42/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCC > minE/2936889‑2937020 | aaTGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCAc > 1:3179395/1‑71 (MQ=255) tGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCAccc < 1:1210818/71‑1 (MQ=255) gagaTCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCAccc > 1:1992501/1‑70 (MQ=255) agaTCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCa < 1:3779169/70‑1 (MQ=255) gaTCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATc < 1:1040009/71‑1 (MQ=255) aTCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATc < 1:1738778/70‑1 (MQ=255) tCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGc < 1:3237511/71‑1 (MQ=255) tCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGc > 1:3897164/1‑71 (MQ=255) cAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGc > 1:2952791/1‑70 (MQ=255) cAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGc > 1:779946/1‑70 (MQ=255) aaaCTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCAccc < 1:2956964/64‑1 (MQ=255) aCTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCAccc > 1:1664144/1‑62 (MQ=255) ccccGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACtt > 1:2213866/1‑71 (MQ=255) ccccGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACtt > 1:2086910/1‑71 (MQ=255) ccccGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACt < 1:1278997/70‑1 (MQ=255) ccccGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACt < 1:967081/70‑1 (MQ=255) cGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGTACCCATCGCCAGCACTTCCt > 1:1396548/1‑71 (MQ=255) gCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCa > 1:3767912/1‑64 (MQ=255) gCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTg < 1:2412325/71‑1 (MQ=255) aGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGTACCCATCGCCAGCACTTCCTGctc > 1:3074931/1‑71 (MQ=255) tGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGctct > 1:3443771/1‑70 (MQ=255) tGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCtt > 1:3468681/1‑71 (MQ=255) tGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCtt < 1:2778974/71‑1 (MQ=255) cTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTc > 1:1198503/1‑71 (MQ=255) tCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGTACCCATCGCCAGCACTTCCTGCTCTTTCGcc > 1:2196710/1‑71 (MQ=255) cAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGTACCCATCGCCAGCACTTCCTg > 1:3210578/1‑60 (MQ=255) cAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGTACCCATCGCCAGCACTTCCTg > 1:2703428/1‑60 (MQ=255) cAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGTACCCATCGCCAGCACTTCCTg > 1:241515/1‑60 (MQ=255) aGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGctct < 1:168303/63‑1 (MQ=255) aGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCgg < 1:1232273/71‑1 (MQ=255) tGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGAt < 1:1440359/71‑1 (MQ=255) tGCCTGCGGATCG‑GGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTcc > 1:1820159/1‑54 (MQ=255) cTGCGGATCGCGGCTATTCACCACTTTATCGGTACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAg < 1:878015/71‑1 (MQ=255) cTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAg < 1:3148180/71‑1 (MQ=255) tGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCttt < 1:3164574/59‑1 (MQ=255) cGGATCGCGGCTATTCACCACTTTATCGGTACCCAt > 1:2511599/1‑36 (MQ=255) cGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCAc > 1:2971166/1‑45 (MQ=255) cGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAAc > 1:1350628/1‑71 (MQ=255) cGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAAc > 1:1926342/1‑71 (MQ=255) ggATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACt < 1:2991788/71‑1 (MQ=255) gATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGAtt < 1:2791710/64‑1 (MQ=255) gATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACt > 1:1956965/1‑70 (MQ=255) gATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACt < 1:867041/70‑1 (MQ=255) aTCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGctct < 1:3705593/52‑1 (MQ=255) aTCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACt < 1:3208700/69‑1 (MQ=255) aTCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACt < 1:2763863/69‑1 (MQ=255) tCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACt > 1:3007244/1‑68 (MQ=255) cgcgGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTaa > 1:955112/1‑69 (MQ=255) cgcgGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAg > 1:1631289/1‑71 (MQ=255) gcgGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAg < 1:57985/70‑1 (MQ=255) cTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGt < 1:1953474/71‑1 (MQ=255) aTTCACGACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCg < 1:3302717/50‑1 (MQ=255) ttCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCAc < 1:935810/71‑1 (MQ=255) tCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAg < 1:1360787/62‑1 (MQ=255) caccacTTTATCGGTACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGaa > 1:1113925/1‑55 (MQ=255) caccacTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTc > 1:2784066/1‑43 (MQ=255) caccacTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCt > 1:3071434/1‑71 (MQ=255) caccacTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCt > 1:2212777/1‑71 (MQ=255) caccacTTTATAGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCt > 1:2424314/1‑71 (MQ=255) ccacTTTATCGGCACCCATCGCCAGCACTTCCTGc < 1:755817/35‑1 (MQ=255) ccacTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCAcc < 1:1736579/68‑1 (MQ=255) ccacTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCAcc < 1:1857520/68‑1 (MQ=255) ccacTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCg < 1:768783/71‑1 (MQ=255) cacTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTaaa < 1:2653490/57‑1 (MQ=255) cacTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCg > 1:1403230/1‑70 (MQ=255) cTTTATCGGTACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAg < 1:1619248/48‑1 (MQ=255) cTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAg < 1:292292/48‑1 (MQ=255) cTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCAt > 1:804335/1‑71 (MQ=255) tttATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTg > 1:3992375/1‑59 (MQ=255) ttATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTg > 1:971870/1‑58 (MQ=255) ttATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATcc > 1:1974883/1‑71 (MQ=255) ttATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATcc > 1:2979167/1‑71 (MQ=255) tATCGGTACCCATCGCCAGCACTTCCTGCTCTTTCGc > 1:2529090/1‑37 (MQ=255) tATCGGTACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAg < 1:3915447/53‑1 (MQ=255) tATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTaa > 1:2326516/1‑51 (MQ=255) tATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTaa > 1:2635518/1‑51 (MQ=255) tATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATcc < 1:1547217/70‑1 (MQ=255) tATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATcc > 1:3324370/1‑70 (MQ=255) | AATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCC > minE/2936889‑2937020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |