| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,840 | C→A | 8.1% | V345F (GTC→TTC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,840 | 0 | C | A | 8.1% | 284.6 / 6.4 | 86 | V345F (GTC→TTC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (35/44); new base A (2/5); total (37/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTT > minE/311770‑311909 | ggAATCAACGTCTCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGAc > 1:3416269/1‑71 (MQ=255) cgcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:3432227/1‑71 (MQ=255) cgcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:706391/1‑71 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:3644790/1‑70 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc < 1:2472535/71‑1 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc > 1:3917369/1‑71 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc > 1:2901924/1‑71 (MQ=255) cgGAAGTTCTGCAGGAACAGATACATAACCCGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc < 1:3786173/71‑1 (MQ=255) ggAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACt > 1:2842000/1‑71 (MQ=255) atattCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTc < 1:4132534/68‑1 (MQ=16) atattCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTc < 1:1257570/68‑1 (MQ=16) attCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGt > 1:3806065/2‑71 (MQ=18) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:3051962/71‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:3151805/71‑1 (MQ=255) cTGCAGGAACAGAAACATAACCAGGAACACGAGGAAGATCGCTTCGACCAGCGTTTTAACCACt > 1:2082536/1‑64 (MQ=255) caagAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGaa < 1:2376885/65‑1 (MQ=21) cAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGaa > 1:3926964/1‑68 (MQ=255) aagAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGaa < 1:3641581/65‑1 (MQ=21) ggAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATagag < 1:3727808/71‑1 (MQ=255) cAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCG‑TTTAACCACTTCGTGAATAGAGAtttt < 1:282269/71‑1 (MQ=255) aGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:1906737/71‑1 (MQ=255) aGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAttt > 1:3224490/1‑70 (MQ=255) gATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:3809363/1‑45 (MQ=255) gATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTc < 1:1770351/71‑1 (MQ=255) aTACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATa > 1:3316468/1‑61 (MQ=255) aTACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:360248/69‑1 (MQ=255) tACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc < 1:3823302/71‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTc < 1:2960730/52‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTc < 1:2870579/52‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTc < 1:1019695/52‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGt < 1:3108121/54‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGt < 1:683765/54‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:2335233/55‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt > 1:2972066/1‑67 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc > 1:4121586/1‑70 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:2657994/53‑1 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:906935/1‑71 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:442460/1‑71 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:66945/1‑71 (MQ=255) tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAc > 1:2112705/1‑71 (MQ=255) tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAc > 1:2195688/1‑71 (MQ=255) aaCCAGGAACACGTGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg > 1:1061898/1‑71 (MQ=255) aCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc > 1:217126/1‑41 (MQ=255) aCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt > 1:3868383/1‑62 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc < 1:618998/39‑1 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc < 1:3437539/39‑1 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATa > 1:2920808/1‑52 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg < 1:1361033/64‑1 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCg < 1:2200701/71‑1 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATagag < 1:86175/52‑1 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc > 1:2062484/1‑60 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtgg > 1:2425559/1‑71 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACTACTTCGTGAATAGAGATTTTCACGAACGTCgtgg < 1:1653084/70‑1 (MQ=255) acacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggtg < 1:2719486/71‑1 (MQ=255) cacGAGGATGATCGCTTCGACCAGCGTTTTAACCAc > 1:2161213/1‑36 (MQ=255) cacGAGGATGATCGCTTCGACCAGCGTTTTAACCAc > 1:2148393/1‑36 (MQ=255) cacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGaa > 1:3436375/1‑44 (MQ=255) cacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggtg < 1:770770/70‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGaa < 1:2813876/42‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:3546563/71‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:1569925/71‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:3830764/71‑1 (MQ=255) gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCg < 1:3222918/62‑1 (MQ=255) gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:2843283/70‑1 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa < 1:3447892/71‑1 (MQ=255) gatgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATag < 1:1701625/41‑1 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:1592630/1‑52 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg < 1:3144583/54‑1 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATggg > 1:2882058/1‑71 (MQ=255) gatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg < 1:2270954/58‑1 (MQ=255) atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGt < 1:2409392/67‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg < 1:2593067/57‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGt > 1:72631/1‑70 (MQ=255) ggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa > 1:1326359/2‑69 (MQ=255) cGCTTCGACCAGCGTTTTAACCACTTCGTGAATAgaga > 1:3200662/1‑38 (MQ=255) cGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTGACGAACGGCgtggt > 1:3754235/1‑57 (MQ=255) cGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt > 1:2418844/1‑57 (MQ=255) gCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:3901708/53‑1 (MQ=255) gCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:2217799/53‑1 (MQ=255) cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg < 1:474611/44‑1 (MQ=255) cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg < 1:1477670/53‑1 (MQ=255) ttCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTaaa > 1:437715/1‑68 (MQ=255) cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAAGGCGTGGTGTCGTATGGGTAAACa > 1:2040389/1‑68 (MQ=255) gACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtt < 1:3340499/70‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:1053984/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:3171169/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtt < 1:1015302/69‑1 (MQ=255) | GGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTT > minE/311770‑311909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |