| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,874 | C→G | 5.7% | V333V (GTG→GTC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,874 | 0 | C | G | 5.7% | 276.6 / 3.7 | 87 | V333V (GTG→GTC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (37/45); new base G (3/2); total (40/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCC > minE/311804‑311943 | gATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTc < 1:1770351/71‑1 (MQ=255) tACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc < 1:3823302/71‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc > 1:4121586/1‑70 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:906935/1‑71 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:66945/1‑71 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:442460/1‑71 (MQ=255) tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAc > 1:2195688/1‑71 (MQ=255) tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAc > 1:2112705/1‑71 (MQ=255) aaCCAGGAACACGTGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg > 1:1061898/1‑71 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg < 1:1361033/64‑1 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCg < 1:2200701/71‑1 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc > 1:2062484/1‑60 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtgg > 1:2425559/1‑71 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACTACTTCGTGAATAGAGATTTTCACGAACGTCgtgg < 1:1653084/70‑1 (MQ=255) acacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggtg < 1:2719486/71‑1 (MQ=255) cacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggtg < 1:770770/70‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:1569925/71‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:3546563/71‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:3830764/71‑1 (MQ=255) gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCg < 1:3222918/62‑1 (MQ=255) gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:2843283/70‑1 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa < 1:3447892/71‑1 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:1592630/1‑52 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg < 1:3144583/54‑1 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATggg > 1:2882058/1‑71 (MQ=255) gatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg < 1:2270954/58‑1 (MQ=255) atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGt < 1:2409392/67‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg < 1:2593067/57‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGt > 1:72631/1‑70 (MQ=255) ggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa > 1:1326359/2‑69 (MQ=255) cGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTGACGAACGGCgtggt > 1:3754235/1‑57 (MQ=255) cGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt > 1:2418844/1‑57 (MQ=255) gCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:2217799/53‑1 (MQ=255) gCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:3901708/53‑1 (MQ=255) cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg < 1:474611/44‑1 (MQ=255) cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg < 1:1477670/53‑1 (MQ=255) ttCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTaaa > 1:437715/1‑68 (MQ=255) cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAAGGCGTGGTGTCGTATGGGTAAACa > 1:2040389/1‑68 (MQ=255) gACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtt < 1:3340499/70‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:3171169/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:1053984/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtt < 1:1015302/69‑1 (MQ=255) cAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCa < 1:2790105/71‑1 (MQ=255) aGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg < 1:1484704/45‑1 (MQ=255) aGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTaaa > 1:3857942/1‑61 (MQ=255) gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:327933/43‑1 (MQ=255) gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:1639191/43‑1 (MQ=255) gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa < 1:75179/71‑1 (MQ=255) cGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATgg > 1:3838680/1‑54 (MQ=255) ttttAACCACTTCGTGAATATAGATTTTCACTAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa < 1:3202357/68‑1 (MQ=255) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:2675163/47‑1 (MQ=255) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAg < 1:1429467/67‑1 (MQ=255) tttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:1285712/63‑1 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt > 1:4012222/1‑41 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt > 1:3785071/1‑41 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTc > 1:2098151/1‑63 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa > 1:187607/1‑71 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa > 1:2175611/1‑71 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa > 1:2213810/1‑71 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa > 1:463632/1‑71 (MQ=255) aCTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:2766196/1‑41 (MQ=255) aCTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAc < 1:420580/61‑1 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag < 1:646312/71‑1 (MQ=255) cTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTAGAGAACCTTCATTCCCTGCGGgaag < 1:2744248/71‑1 (MQ=255) ttCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:888904/54‑1 (MQ=255) tCGTGAATAGAGATTTTCACGATCGGCGTGGTGTCGTa > 1:61539/1‑38 (MQ=255) tCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa > 1:651076/1‑49 (MQ=255) tCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTGGAGAACCTTCATTCCCTGCGGgaagaa > 1:3759953/1‑71 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:1043407/1‑37 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:1077709/1‑37 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:3795534/1‑37 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTc > 1:1099397/1‑53 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag < 1:3793707/68‑1 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAAc > 1:110222/1‑71 (MQ=255) taaaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAAc < 1:1177759/67‑1 (MQ=255) tattAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACgg < 1:4104252/67‑1 (MQ=255) aaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAt < 1:3500427/45‑1 (MQ=255) aaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaagaa > 1:2007828/1‑66 (MQ=255) tAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt > 1:2761428/1‑45 (MQ=255) tAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTcc < 1:467296/71‑1 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa > 1:3675489/1‑40 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa > 1:2458169/1‑40 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt < 1:985052/71‑1 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt < 1:1438278/71‑1 (MQ=255) agaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:1985641/43‑1 (MQ=255) agaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:2620911/43‑1 (MQ=255) agaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATc < 1:4053385/71‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAA‑AAtttt > 1:401097/1‑39 (MQ=25) cACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGcc < 1:222910/70‑1 (MQ=255) | GATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCC > minE/311804‑311943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |