| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,895 | T→A | 6.4% | P326P (CCA→CCT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,895 | 0 | T | A | 6.4% | 169.6 / 2.1 | 60 | P326P (CCA→CCT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (25/31); new base A (2/2); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCG > minE/311828‑311962 | tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATggg > 1:2882058/1‑71 (MQ=255) atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGt < 1:2409392/67‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGt > 1:72631/1‑70 (MQ=255) ggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa > 1:1326359/2‑69 (MQ=255) ttCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTaaa > 1:437715/1‑68 (MQ=255) cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAAGGCGTGGTGTCGTATGGGTAAACa > 1:2040389/1‑68 (MQ=255) gACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtt < 1:3340499/70‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:1053984/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:3171169/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtt < 1:1015302/69‑1 (MQ=255) cAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCa < 1:2790105/71‑1 (MQ=255) aGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTaaa > 1:3857942/1‑61 (MQ=255) gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa < 1:75179/71‑1 (MQ=255) cGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATgg > 1:3838680/1‑54 (MQ=255) ttttAACCACTTCGTGAATATAGATTTTCACTAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa < 1:3202357/68‑1 (MQ=255) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAg < 1:1429467/67‑1 (MQ=255) tttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:1285712/63‑1 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTc > 1:2098151/1‑63 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa > 1:2213810/1‑71 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa > 1:463632/1‑71 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa > 1:2175611/1‑71 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa > 1:187607/1‑71 (MQ=255) aCTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAc < 1:420580/61‑1 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag < 1:646312/71‑1 (MQ=255) cTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTAGAGAACCTTCATTCCCTGCGGgaag < 1:2744248/71‑1 (MQ=255) ttCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:888904/54‑1 (MQ=255) tCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa > 1:651076/1‑49 (MQ=255) tCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTGGAGAACCTTCATTCCCTGCGGgaagaa > 1:3759953/1‑71 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTc > 1:1099397/1‑53 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag < 1:3793707/68‑1 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAAc > 1:110222/1‑71 (MQ=255) taaaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAAc < 1:1177759/67‑1 (MQ=255) tattAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACgg < 1:4104252/67‑1 (MQ=255) aaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAt < 1:3500427/45‑1 (MQ=255) aaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaagaa > 1:2007828/1‑66 (MQ=255) tAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt > 1:2761428/1‑45 (MQ=255) tAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTcc < 1:467296/71‑1 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa > 1:2458169/1‑40 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa > 1:3675489/1‑40 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt < 1:985052/71‑1 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt < 1:1438278/71‑1 (MQ=255) agaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:1985641/43‑1 (MQ=255) agaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:2620911/43‑1 (MQ=255) agaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATc < 1:4053385/71‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAA‑AAtttt > 1:401097/1‑39 (MQ=25) cACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGcc < 1:222910/70‑1 (MQ=255) aCGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAg < 1:3123314/71‑1 (MQ=255) aCGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAg < 1:2897831/71‑1 (MQ=255) aCGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAg > 1:2083655/1‑71 (MQ=255) cGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGtt > 1:3323121/1‑57 (MQ=255) gtggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACg < 1:2385732/71‑1 (MQ=255) gtggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATATTCGCCAGTTCAGCACg < 1:2857905/71‑1 (MQ=255) tggtgTCGTATGTGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACg < 1:1825211/70‑1 (MQ=255) tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt > 1:1002855/1‑49 (MQ=255) tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGAtt > 1:1493013/1‑71 (MQ=255) tCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGc < 1:4060135/53‑1 (MQ=255) cGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTc < 1:2132783/43‑1 (MQ=255) gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCa < 1:3163284/61‑1 (MQ=255) gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCa > 1:3865418/1‑61 (MQ=255) aTGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCg > 1:2562262/1‑69 (MQ=255) | TGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCG > minE/311828‑311962 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |