| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,534 | C→G | 11.2% | L113L (CTG→CTC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,534 | 0 | C | G | 11.2% | 229.8 / 15.5 | 80 | L113L (CTG→CTC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (37/34); new base G (6/3); total (43/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01 | |||||||||||
AGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGA > minE/312467‑312604 | aGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGccct > 1:2937306/1‑68 (MQ=255) ctgctgTATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTg < 1:2824893/71‑1 (MQ=255) ctgctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTg < 1:1574427/71‑1 (MQ=255) tgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTg < 1:46484/70‑1 (MQ=255) tgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTg < 1:2469988/70‑1 (MQ=255) tgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGt < 1:2443771/71‑1 (MQ=255) tgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGt < 1:263968/71‑1 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGtt < 1:3458726/70‑1 (MQ=255) tctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTg < 1:3633194/70‑1 (MQ=255) ctcAACGCTCACCCCTTGCTGCTGGACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCa > 1:3768052/1‑71 (MQ=255) ctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc < 1:1285477/70‑1 (MQ=255) ctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc < 1:2309462/70‑1 (MQ=255) ctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCa > 1:3244722/1‑71 (MQ=255) aaCGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCt > 1:737035/1‑71 (MQ=255) aCGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTg < 1:2484024/71‑1 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTg > 1:1001038/1‑52 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:3679459/71‑1 (MQ=255) gatccccTGCTGCTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCAcc > 1:2370368/4‑64 (MQ=255) cACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGtt > 1:2061855/1‑57 (MQ=255) ccccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgc < 1:3909276/71‑1 (MQ=255) ccccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgc > 1:3584377/1‑71 (MQ=255) ccccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgc > 1:2161056/1‑71 (MQ=255) cTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:144054/1‑70 (MQ=255) ttGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGttt < 1:2385447/48‑1 (MQ=255) tgctgcTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTg < 1:983296/48‑1 (MQ=255) tgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTg < 1:2691196/48‑1 (MQ=19) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCt > 1:2949376/1‑51 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat < 1:1439362/70‑1 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACATGCGCGata > 1:4121072/1‑71 (MQ=255) gctgctGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGt > 1:4055741/1‑44 (MQ=16) gctgctGAACATCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGt > 1:1610271/1‑44 (MQ=16) ctgctgAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGat < 1:3652408/69‑1 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATcc > 1:3016428/1‑71 (MQ=255) ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:3629191/1‑57 (MQ=255) ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat < 1:882196/66‑1 (MQ=255) ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCg > 1:2171676/1‑71 (MQ=255) gAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg < 1:510482/58‑1 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc < 1:1552607/69‑1 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt < 1:3180559/71‑1 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCAc > 1:3997405/1‑47 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:4026884/1‑70 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat > 1:1272280/1‑61 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat > 1:2182012/1‑61 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat > 1:1338591/1‑61 (MQ=255) ttcttGCGGCAGCAACGGCATCGCCAGCTGCAGtt < 1:3660103/35‑1 (MQ=255) tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg > 1:35390/1‑70 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:1235457/1‑49 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:1240221/1‑49 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa > 1:92574/1‑71 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:2360078/1‑69 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:1210264/1‑69 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:3028572/1‑69 (MQ=255) cGGCATCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg < 1:737645/70‑2 (MQ=12) cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCa < 1:1260947/70‑1 (MQ=255) gcagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc < 1:304415/71‑1 (MQ=255) gcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg > 1:2110872/1‑69 (MQ=12) gcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg > 1:3535914/1‑69 (MQ=12) gcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg > 1:3531102/1‑69 (MQ=12) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGata < 1:1416572/50‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:2788957/71‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:3570354/71‑1 (MQ=255) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:3336376/71‑1 (MQ=255) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:2376829/1‑71 (MQ=255) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:2351372/1‑71 (MQ=255) cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTTAACCTGCGCGATATCCGCAt < 1:791388/53‑1 (MQ=255) gggaTCGACAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:3347239/68‑1 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:2990753/62‑1 (MQ=255) cATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:3728487/1‑69 (MQ=255) cATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:2012322/1‑69 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:2178774/71‑1 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:841145/71‑1 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:1578154/71‑1 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg > 1:1257401/1‑70 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg < 1:3389683/69‑1 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3393173/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3519374/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3957681/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3445840/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:1535932/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:978908/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCACGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:2122566/1‑71 (MQ=255) ccAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:3038001/71‑1 (MQ=255) ccAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:1073045/71‑1 (MQ=255) cAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGatat > 1:1442397/1‑38 (MQ=255) cAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa > 1:2646272/1‑71 (MQ=255) cAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa > 1:3511589/1‑71 (MQ=255) | AGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGA > minE/312467‑312604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |