| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,561 | C→T | 8.2% | Q104Q (CAG→CAA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,561 | 0 | C | T | 8.2% | 360.6 / 14.9 | 110 | Q104Q (CAG→CAA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (59/41); new base T (3/6); total (63/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
CCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGT > minE/312494‑312631 | ccccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgc > 1:2161056/1‑71 (MQ=255)ccccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgc > 1:3584377/1‑71 (MQ=255)ccccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgc < 1:3909276/71‑1 (MQ=255) cTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:144054/1‑70 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat < 1:1439362/70‑1 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACATGCGCGata > 1:4121072/1‑71 (MQ=255) ctgctgAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGat < 1:3652408/69‑1 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATcc > 1:3016428/1‑71 (MQ=255) ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat < 1:882196/66‑1 (MQ=255) ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCg > 1:2171676/1‑71 (MQ=255) gAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg < 1:510482/58‑1 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt < 1:3180559/71‑1 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:4026884/1‑70 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat > 1:2182012/1‑61 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat > 1:1338591/1‑61 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat > 1:1272280/1‑61 (MQ=255) tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg > 1:35390/1‑70 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa > 1:92574/1‑71 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:3028572/1‑69 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:1210264/1‑69 (MQ=255) cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCa < 1:1260947/70‑1 (MQ=255) gcagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc < 1:304415/71‑1 (MQ=255) gcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg > 1:3531102/1‑69 (MQ=12) gcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg > 1:3535914/1‑69 (MQ=12) gcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg > 1:2110872/1‑69 (MQ=12) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGata < 1:1416572/50‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:3570354/71‑1 (MQ=255) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:3336376/71‑1 (MQ=255) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:2351372/1‑71 (MQ=255) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:2376829/1‑71 (MQ=255) cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTTAACCTGCGCGATATCCGCAt < 1:791388/53‑1 (MQ=255) gggaTCGACAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:3347239/68‑1 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:2990753/62‑1 (MQ=255) cATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:3728487/1‑69 (MQ=255) cATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:2012322/1‑69 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:841145/71‑1 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:2178774/71‑1 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:1578154/71‑1 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg > 1:1257401/1‑70 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg < 1:3389683/69‑1 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3519374/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:1535932/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3393173/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3957681/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCACGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:2122566/1‑71 (MQ=255) ccAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:1073045/71‑1 (MQ=255) cAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGatat > 1:1442397/1‑38 (MQ=255) cAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa > 1:3511589/1‑71 (MQ=255) cAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa > 1:2646272/1‑71 (MQ=255) aaGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa > 1:3665785/2‑71 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:2523077/1‑71 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGAATCAGTACCAGACTCAGAGGTCAGGGTGAt > 1:3466175/1‑71 (MQ=255) aGCTGCAGTTTGTTATGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:3491305/1‑71 (MQ=255) cTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa < 1:3258854/51‑1 (MQ=12) cTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa < 1:734976/51‑1 (MQ=12) cTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa < 1:1655155/51‑1 (MQ=12) cTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa < 1:1887770/51‑1 (MQ=12) cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:1203974/67‑1 (MQ=255) cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:3189015/1‑69 (MQ=255) cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGAATCAGTACCAGACTCAAAGGTCAGGGTGATCt > 1:2820966/1‑71 (MQ=255) cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCAGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:318973/1‑69 (MQ=255) tGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAtg < 1:3866301/41‑2 (MQ=255) tGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg > 1:3588475/1‑71 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:3910271/60‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:3822765/69‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:2009352/69‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:2848358/69‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:3802011/69‑1 (MQ=255) aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc < 1:2507194/71‑1 (MQ=255) gTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACg > 1:1432303/1‑71 (MQ=255) gTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACg > 1:3967590/1‑71 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg < 1:2998188/53‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:1632188/54‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:2936538/54‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:633519/54‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:1354870/54‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:978370/61‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACgg < 1:3506615/71‑1 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:509066/1‑50 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:3636047/1‑61 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:674896/1‑61 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa < 1:625446/66‑1 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:1927374/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:2535739/1‑71 (MQ=255) ttCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAcc > 1:3602160/1‑71 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa > 1:507193/1‑43 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg > 1:945818/1‑71 (MQ=255) tGCACCTGAAGCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGt > 1:1220312/1‑71 (MQ=255) gCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg > 1:2641954/1‑71 (MQ=255) cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAg > 1:1042034/1‑70 (MQ=255) cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt > 1:1779245/1‑71 (MQ=255) cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt > 1:1760959/1‑71 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGGACCCGTGGAGt > 1:765945/1‑70 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCaa > 1:138925/1‑36 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:2510550/1‑71 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:3037525/1‑71 (MQ=255) aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg < 1:922801/64‑1 (MQ=255) aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc < 1:4016983/69‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt < 1:2621067/71‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:1798092/1‑71 (MQ=255) | CCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGT > minE/312494‑312631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |