| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,585 | A→G | 5.3% | S96S (TCT→TCC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,585 | 0 | A | G | 5.3% | 390.7 / 10.0 | 132 | S96S (TCT→TCC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (67/58); new base G (3/4); total (70/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
CGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAT > minE/312516‑312655 | cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCa < 1:1260947/70‑1 (MQ=255) gcagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc < 1:304415/71‑1 (MQ=255) gcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg > 1:3535914/1‑69 (MQ=12) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:2351372/1‑71 (MQ=255) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:2376829/1‑71 (MQ=255) gggaTCGACAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:3347239/68‑1 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:2990753/62‑1 (MQ=255) cATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:3728487/1‑69 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:841145/71‑1 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:2178774/71‑1 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:1578154/71‑1 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg > 1:1257401/1‑70 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg < 1:3389683/69‑1 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3957681/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:1535932/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3445840/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:3519374/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCACGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:2122566/1‑71 (MQ=255) ccAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:3038001/71‑1 (MQ=255) ccAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:1073045/71‑1 (MQ=255) cAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa > 1:2646272/1‑71 (MQ=255) cAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa > 1:3511589/1‑71 (MQ=255) aaGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa > 1:3665785/2‑71 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:2523077/1‑71 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGAATCAGTACCAGACTCAGAGGTCAGGGTGAt > 1:3466175/1‑71 (MQ=255) aGCTGCAGTTTGTTATGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:3491305/1‑71 (MQ=255) cTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa < 1:734976/51‑1 (MQ=12) cTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa < 1:1887770/51‑1 (MQ=12) cTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa < 1:1655155/51‑1 (MQ=12) cTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa < 1:3258854/51‑1 (MQ=12) cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:1203974/67‑1 (MQ=255) cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:3189015/1‑69 (MQ=255) cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGAATCAGTACCAGACTCAAAGGTCAGGGTGATCt > 1:2820966/1‑71 (MQ=255) tGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg > 1:3588475/1‑71 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg < 1:3910271/60‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:3822765/69‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:2009352/69‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:2848358/69‑1 (MQ=255) gTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACg > 1:1432303/1‑71 (MQ=255) gTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACg > 1:3967590/1‑71 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg < 1:2998188/53‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:633519/54‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:2936538/54‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:978370/61‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCAGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:3755505/61‑1 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:509066/1‑50 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa > 1:3556662/1‑54 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:674896/1‑61 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa < 1:625446/66‑1 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:1927374/1‑71 (MQ=255) ttCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAcc > 1:3602160/1‑71 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa > 1:507193/1‑43 (MQ=255) cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAg > 1:1042034/1‑70 (MQ=255) cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt > 1:1760959/1‑71 (MQ=255) cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt > 1:1779245/1‑71 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCaa > 1:138925/1‑36 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:2510550/1‑71 (MQ=255) aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg < 1:922801/64‑1 (MQ=255) aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc < 1:4016983/69‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt < 1:2621067/71‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:1798092/1‑71 (MQ=255) tGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCAc < 1:2003998/67‑1 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:813574/1‑69 (MQ=255) cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGATTCACTGTTag < 1:2926808/71‑1 (MQ=255) cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTa < 1:1194559/70‑1 (MQ=255) cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTa < 1:932816/70‑1 (MQ=255) cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGGGATCTGCACGGTACCCGTGGAGTCACTGTTag < 1:2374867/71‑1 (MQ=255) cgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc < 1:1127175/61‑1 (MQ=255) atatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTag < 1:2601741/67‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACgg < 1:2038381/45‑1 (MQ=255) atCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg > 1:3374315/1‑50 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:2764503/1‑35 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt > 1:90525/1‑35 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGa < 1:842902/53‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACa < 1:2803921/70‑1 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg > 1:3645555/1‑70 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg > 1:2737985/1‑70 (MQ=255) gCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa < 1:1712299/71‑1 (MQ=255) cATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTAc < 1:4011723/71‑1 (MQ=255) aTCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACa > 1:3569084/1‑65 (MQ=255) aTCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTAc > 1:3707430/1‑70 (MQ=255) tCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACAt < 1:3984926/71‑1 (MQ=255) tCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACAt < 1:1892127/71‑1 (MQ=255) cAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt < 1:3005516/54‑1 (MQ=255) cAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACAt > 1:2086060/1‑70 (MQ=255) aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc > 1:4122432/1‑48 (MQ=255) aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTa > 1:1674557/1‑55 (MQ=255) aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa > 1:951507/1‑66 (MQ=255) aCCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACt < 1:2519534/48‑1 (MQ=255) aCCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGt > 1:1452009/1‑71 (MQ=255) ccAGACTCAAAGGTCAGGGTGATCTTCACGGTACCCGTGGAGTCACTGTTag < 1:703865/52‑1 (MQ=255) ccAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:3242456/1‑49 (MQ=255) ccAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:3237600/1‑49 (MQ=255) ccAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTag < 1:3831042/52‑1 (MQ=255) ccAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTag < 1:2066498/52‑1 (MQ=255) ccAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGtt < 1:1006709/71‑1 (MQ=255) cAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt < 1:1239490/48‑1 (MQ=255) cAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg < 1:3607766/59‑1 (MQ=255) aGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt < 1:407650/47‑1 (MQ=255) aGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAt > 1:217562/1‑71 (MQ=255) | CGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAT > minE/312516‑312655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |