| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,184 | G→T | 9.2% | V227V (GTG→GTT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,184 | 0 | G | T | 9.2% | 209.6 / 5.0 | 65 | V227V (GTG→GTT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (32/27); new base T (2/4); total (34/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01 | |||||||||||
CGCGCGGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCAT > minE/189115‑189253 | cgcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTgg > 1:2237304/1‑71 (MQ=255) gcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTggg < 1:936190/71‑1 (MQ=255) gcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTggg < 1:351132/71‑1 (MQ=255) gcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTggg > 1:369627/1‑71 (MQ=255) cgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGt < 1:3860261/71‑1 (MQ=255) cgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGt < 1:1815588/71‑1 (MQ=255) ggACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg < 1:3694745/71‑1 (MQ=255) cTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAc > 1:1635857/1‑71 (MQ=255) ttttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcg > 1:427410/1‑71 (MQ=255) ttttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcg > 1:358159/1‑71 (MQ=255) ttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCTACGTCGAACAAAATTGTGGGTATGTAcgcc > 1:3890852/1‑71 (MQ=255) ttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgc > 1:1267283/1‑70 (MQ=255) gcgcGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgccgc < 1:672161/70‑1 (MQ=255) gcgcGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgccgc < 1:2816228/70‑1 (MQ=255) cgGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg < 1:867325/59‑1 (MQ=255) gAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg < 1:2392419/57‑1 (MQ=255) gAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg < 1:2837987/57‑1 (MQ=255) gAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg < 1:1445493/57‑1 (MQ=255) cAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCa > 1:2087194/1‑66 (MQ=255) gTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCAt > 1:767364/1‑63 (MQ=255) gTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCAt > 1:706291/1‑63 (MQ=255) gTTATATCGTTACCAACGGAACATCGACGTCGAACAAAAT‑‑TGGGTATGTACGCCGCGCCAt > 1:2869515/1‑61 (MQ=255) ttATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTgg < 1:2498662/44‑1 (MQ=255) ttATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTa < 1:4077573/47‑1 (MQ=255) tataTCGTTACCAACGGAACATCGACGTCGAACAAAATtgtg > 1:4060671/1‑42 (MQ=255) atatCGTTACCAACGGAACATCGACGTCGAACAAAATTGTgg > 1:747902/1‑42 (MQ=255) atatCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgccgc > 1:3692682/1‑55 (MQ=255) atatCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACg > 1:3513510/1‑71 (MQ=255) tatCGTTACCAACGTAACATCGACGTCGAACAAAATTGTGGGTa < 1:3219928/44‑1 (MQ=255) tatCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGt < 1:2192168/43‑1 (MQ=255) atCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg < 1:401639/45‑1 (MQ=255) cGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGt > 1:2555742/1‑71 (MQ=255) cGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGt > 1:653708/1‑71 (MQ=255) ccAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc < 1:2727966/71‑1 (MQ=255) ccAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc < 1:1946526/71‑1 (MQ=255) cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:164692/71‑1 (MQ=255) aaCGGTACTTCCACTGCGAACAAAATTGTTGGTATGTACTCTGCTCCag < 1:1047279/49‑2 (MQ=255) aCGGAACATCGACGTCGAACAAAATTGTGGGTATGt > 1:395519/1‑36 (MQ=255) gAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCg > 1:1014092/1‑70 (MQ=255) aaCATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGc > 1:1378356/1‑70 (MQ=255) aTCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTg < 1:1098583/58‑1 (MQ=255) tCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCt > 1:3628666/1‑53 (MQ=255) tCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGa < 1:1796754/58‑1 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATc > 1:1086150/1‑37 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCaa > 1:1885878/1‑64 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAt > 1:3197765/1‑65 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAt > 1:797214/1‑65 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCa > 1:3904913/1‑70 (MQ=255) gTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCAt > 1:3034066/1‑70 (MQ=255) tCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa < 1:2037503/40‑1 (MQ=255) tCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaa < 1:2770815/71‑1 (MQ=255) gcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTaac > 1:2405916/2‑62 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCaca < 1:3512941/69‑3 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCaca < 1:273590/69‑3 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaaa > 1:1401832/1‑71 (MQ=255) cGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa > 1:3220448/1‑39 (MQ=255) cGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaaa > 1:3138174/1‑71 (MQ=255) gAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACAAAt < 1:2307331/71‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGt > 1:2458660/1‑40 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTa > 1:2325206/1‑41 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc < 1:2889164/52‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:1743851/1‑71 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:1477894/1‑71 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:1496904/1‑71 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:3339934/1‑71 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt < 1:3273680/70‑1 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:496206/71‑1 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3982843/71‑1 (MQ=255) aaaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:3631015/48‑1 (MQ=255) aTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTgg < 1:1411818/70‑1 (MQ=255) aTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGgc > 1:2122675/1‑71 (MQ=255) aTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGgc < 1:2569125/71‑1 (MQ=255) tgGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCAt > 1:1077841/1‑71 (MQ=255) | CGCGCGGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCAT > minE/189115‑189253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |