| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 313,801 | A→G | 7.2% | I96I (ATT→ATC) | acrA ← | multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 313,801 | 0 | A | G | 7.2% | 238.7 / 8.6 | 84 | I96I (ATT→ATC) | acrA | multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base A (41/36); new base G (3/3); total (44/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCG > minE/313732‑313870 | ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa > 1:3447952/1‑71 (MQ=255)ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa < 1:2953146/70‑1 (MQ=255)ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa < 1:3812138/70‑1 (MQ=255) cAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGa < 1:2514709/71‑1 (MQ=255) ccTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGa < 1:3646586/68‑1 (MQ=255) cTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa > 1:2684716/1‑69 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa < 1:999900/60‑1 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa < 1:430368/60‑1 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCATTCTGATa > 1:1592507/1‑68 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAt > 1:575804/1‑67 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAAt > 1:3178778/1‑60 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAAt > 1:3545509/1‑60 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga > 1:1097008/1‑68 (MQ=255) gCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga < 1:2717014/71‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag < 1:3297119/66‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag < 1:1907769/66‑1 (MQ=255) ttCGCCAGATCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg > 1:1811369/1‑68 (MQ=255) ttCGCCAGATCACCTTTCGCACTGTCGTATGTCCCCTGATAGGTCGCAGGATCAATCTGATAgagagaga > 1:2040572/1‑70 (MQ=255) tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAgagagaga < 1:316824/69‑1 (MQ=255) tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAgagagaga < 1:2229540/69‑1 (MQ=255) tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGac < 1:1184254/70‑1 (MQ=255) tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGac < 1:3670336/70‑1 (MQ=255) tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGac < 1:1322179/70‑1 (MQ=255) gCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:4120862/60‑1 (MQ=255) cAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGa < 1:1232969/55‑1 (MQ=255) cAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAgagagaga > 1:3914454/1‑65 (MQ=255) cAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCt > 1:514151/1‑70 (MQ=255) gATCACCTTTCGCACTGTCGTATGTCGCCTTATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCt < 1:441301/71‑1 (MQ=255) gATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCt < 1:2203957/71‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCt < 1:3090864/50‑1 (MQ=255) tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc < 1:2338972/71‑1 (MQ=255) tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc < 1:536999/71‑1 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag > 1:775562/1‑53 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc > 1:713602/1‑70 (MQ=255) ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCg < 1:2225833/69‑1 (MQ=255) cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATg < 1:2848394/71‑1 (MQ=255) ttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTg < 1:2144366/44‑1 (MQ=255) ttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTg < 1:1586394/44‑1 (MQ=255) ttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTg < 1:3671282/44‑1 (MQ=255) ttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTc > 1:1162506/1‑71 (MQ=255) ttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTc > 1:1552036/1‑71 (MQ=255) tCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTa > 1:1278226/1‑46 (MQ=255) cgcgCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTggt > 1:1986979/1‑42 (MQ=255) cACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa < 1:2013728/36‑1 (MQ=255) cACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa < 1:2159283/36‑1 (MQ=255) aCTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCt > 1:1316272/1‑55 (MQ=255) aCTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCt > 1:3968059/1‑55 (MQ=255) cTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATc > 1:4027660/1‑36 (MQ=255) cTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTAcc > 1:2066732/1‑71 (MQ=255) cTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTAc > 1:3722110/1‑70 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCTATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTa < 1:428906/68‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCg < 1:2301160/59‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTa < 1:1771386/68‑1 (MQ=255) tCGTATGTCGCCTGATAGGTCTCAGGATCAATCTGATAGAGAGAGACACCTGCTTc < 1:1791863/56‑1 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTa > 1:3876393/1‑66 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttc > 1:1580177/1‑71 (MQ=255) gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa > 1:2030076/1‑36 (MQ=255) gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttctt > 1:1450486/1‑71 (MQ=255) tATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCttt > 1:1451079/1‑71 (MQ=255) tATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCttt < 1:2620215/71‑1 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATagagagag > 1:1421552/1‑40 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga > 1:858950/1‑37 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGc > 1:2204003/1‑59 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTg > 1:1325165/1‑70 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa < 1:1259639/71‑1 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa < 1:2436995/71‑1 (MQ=255) tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCttt < 1:434208/67‑1 (MQ=255) cGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGt > 1:4047242/1‑53 (MQ=255) gCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGc > 1:1688524/1‑55 (MQ=255) ccTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGa > 1:2331952/1‑48 (MQ=255) tGATAGGTCGCAGGATCAATCTGATAGAGAGAGac < 1:2989797/35‑1 (MQ=255) gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGc > 1:86362/1‑71 (MQ=255) aTAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa < 1:2817295/62‑1 (MQ=255) aTAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTa > 1:2662533/1‑67 (MQ=255) tAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCt > 1:3936640/1‑70 (MQ=255) ggTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTc < 1:225172/70‑1 (MQ=255) ggTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCa < 1:3618414/71‑1 (MQ=255) gCAGGATCAATCTGATAGAGAGAGACACCTTCTTCGAt < 1:1585523/38‑1 (MQ=255) gCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGAt < 1:2488304/38‑1 (MQ=255) ggATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAAt > 1:3438701/1‑71 (MQ=255) ggATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAAt > 1:272985/1‑71 (MQ=255) gATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa < 1:3633657/51‑1 (MQ=255) gATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa < 1:2715499/51‑1 (MQ=255) gATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCtt < 1:1619768/61‑1 (MQ=255) gATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATc > 1:2637854/1‑71 (MQ=255) gATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATc > 1:2941385/1‑71 (MQ=255) aacAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAAt < 1:338849/51‑1 (MQ=255) aTCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTAc > 1:1366369/1‑41 (MQ=255) aTCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTAc > 1:1320875/1‑41 (MQ=255) tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGAt > 1:218816/1‑65 (MQ=255) tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATccc > 1:913302/1‑71 (MQ=255) aaTCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCg < 1:322994/70‑1 (MQ=255) | GGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCG > minE/313732‑313870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |