| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 337,128 | G→T | 17.0% | intergenic (+100/+216) | ybaQ → / ← copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 337,128 | 0 | G | T | 17.0% | 133.2 / 10.8 | 47 | intergenic (+100/+216) | ybaQ/copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Reads supporting (aligned to +/- strand): ref base G (20/19); new base T (3/5); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01 | |||||||||||
GGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCAT > minE/337058‑337198 | ggATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAg < 1:2520453/71‑1 (MQ=39) gCGTTCACTCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACa < 1:1385624/71‑1 (MQ=255) ttCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACaa < 1:1414843/69‑1 (MQ=40) cACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACtt > 1:208545/1‑71 (MQ=39) cgccgcATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtgt < 1:2774818/71‑1 (MQ=39) gccgcATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtgt < 1:2290489/70‑1 (MQ=40) ccgcATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGc < 1:1908381/71‑1 (MQ=39) gcatccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCg > 1:1304859/1‑70 (MQ=40) ccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGc > 1:1036875/1‑71 (MQ=39) cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtgt > 1:3246959/1‑55 (MQ=255) cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGcc > 1:300237/1‑71 (MQ=255) cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGc < 1:286048/70‑1 (MQ=255) aGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCgg < 1:2826427/71‑1 (MQ=255) gCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAAcc > 1:4136104/1‑61 (MQ=255) gCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCg > 1:1792256/1‑62 (MQ=255) gCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGa < 1:4024984/71‑1 (MQ=255) cACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtg > 1:782387/1‑50 (MQ=255) cACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtg > 1:1598147/1‑50 (MQ=255) cACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGAt < 1:1640671/71‑1 (MQ=255) aCCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGc < 1:3707386/65‑1 (MQ=255) aTTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAgg > 1:3480069/1‑71 (MQ=255) aTTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAg > 1:494883/1‑70 (MQ=255) ttGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTAc > 1:3853705/1‑38 (MQ=40) ttGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGa < 1:2518887/65‑1 (MQ=255) ttccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtgt < 1:3213249/43‑1 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGcc > 1:2589232/1‑47 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGcc > 1:1331733/1‑47 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTa > 1:4001905/1‑56 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATa > 1:2111306/1‑65 (MQ=255) ccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt < 1:2578733/71‑1 (MQ=39) cTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt > 1:123200/1‑70 (MQ=40) gATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtgtg < 1:441281/41‑1 (MQ=255) cGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt < 1:4044008/64‑1 (MQ=40) aCGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgca < 1:2595084/71‑1 (MQ=39) tACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccgg > 1:2480968/1‑71 (MQ=39) tACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccgg > 1:3004984/1‑71 (MQ=39) cGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCgcatccggcagt < 1:3735837/71‑3 (MQ=11) tCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCAcgccg < 1:4045133/58‑1 (MQ=11) tCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCAcgccg < 1:541952/58‑1 (MQ=11) tCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCg < 1:1005731/40‑1 (MQ=18) ttATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATaa > 1:3022205/1‑43 (MQ=14) ttATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGa > 1:129888/1‑40 (MQ=18) ttATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAg > 1:2326016/1‑71 (MQ=255) tATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGttc > 1:2213155/1‑48 (MQ=11) tCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCAcgcc < 1:1979013/52‑1 (MQ=11) tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAc < 1:2318689/71‑1 (MQ=255) cAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGc < 1:2027619/68‑1 (MQ=255) aGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAcc < 1:2605495/70‑1 (MQ=255) ggCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa > 1:2810483/1‑67 (MQ=255) ggCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa > 1:2805773/1‑67 (MQ=255) ggCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCAt < 1:4016339/71‑1 (MQ=255) | GGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCAT > minE/337058‑337198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |