| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 337,146 | T→G | 10.9% | intergenic (+118/+198) | ybaQ → / ← copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 337,146 | 0 | T | G | 10.9% | 126.3 / 14.1 | 55 | intergenic (+118/+198) | ybaQ/copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Reads supporting (aligned to +/- strand): ref base T (26/23); new base G (2/4); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACG > minE/337083‑337212 | gcatccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCg > 1:1304859/1‑70 (MQ=40) ccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGc > 1:1036875/1‑71 (MQ=39) cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGcc > 1:300237/1‑71 (MQ=255) cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGc < 1:286048/70‑1 (MQ=255) aGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCgg < 1:2826427/71‑1 (MQ=255) gCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAAcc > 1:4136104/1‑61 (MQ=255) gCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCg > 1:1792256/1‑62 (MQ=255) gCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGa < 1:4024984/71‑1 (MQ=255) cACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGAt < 1:1640671/71‑1 (MQ=255) aCCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGc < 1:3707386/65‑1 (MQ=255) aTTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAgg > 1:3480069/1‑71 (MQ=255) aTTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAg > 1:494883/1‑70 (MQ=255) ttGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGa < 1:2518887/65‑1 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTa > 1:4001905/1‑56 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATa > 1:2111306/1‑65 (MQ=255) ccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt < 1:2578733/71‑1 (MQ=39) cTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt > 1:123200/1‑70 (MQ=40) cGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt < 1:4044008/64‑1 (MQ=40) aCGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgca < 1:2595084/71‑1 (MQ=39) tACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccgg > 1:3004984/1‑71 (MQ=39) tACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccgg > 1:2480968/1‑71 (MQ=39) cGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCgcatccggcagt < 1:3735837/71‑3 (MQ=11) tCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCAcgccg < 1:4045133/58‑1 (MQ=11) tCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCAcgccg < 1:541952/58‑1 (MQ=11) tCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCg < 1:1005731/40‑1 (MQ=18) ttATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATaa > 1:3022205/1‑43 (MQ=14) ttATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGa > 1:129888/1‑40 (MQ=18) ttATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAg > 1:2326016/1‑71 (MQ=255) tATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGttc > 1:2213155/1‑48 (MQ=11) tCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCAcgcc < 1:1979013/52‑1 (MQ=11) tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAc < 1:2318689/71‑1 (MQ=255) cAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGc < 1:2027619/68‑1 (MQ=255) aGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAcc < 1:2605495/70‑1 (MQ=255) ggCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa > 1:2810483/1‑67 (MQ=255) ggCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa > 1:2805773/1‑67 (MQ=255) ggCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCAt < 1:4016339/71‑1 (MQ=255) gCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCAt > 1:2053727/1‑70 (MQ=255) ccTACAAACTTGTGCCTGAACGGTAGGACGGATAAgg > 1:3945881/1‑37 (MQ=14) ccTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTa > 1:3800090/1‑43 (MQ=14) tACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt < 1:1453513/39‑1 (MQ=40) tACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGtt < 1:1825543/39‑1 (MQ=14) tACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCgca < 1:1788757/48‑1 (MQ=11) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTa > 1:102917/1‑39 (MQ=40) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatc > 1:1808571/1‑48 (MQ=38) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCt > 1:21914/1‑70 (MQ=255) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCt < 1:1752473/70‑1 (MQ=255) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCt > 1:2976338/1‑70 (MQ=255) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCt < 1:1419618/70‑1 (MQ=255) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTCCCTg < 1:3968937/71‑1 (MQ=255) aaaCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGa < 1:107642/71‑1 (MQ=255) aaCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCAt > 1:1870416/1‑63 (MQ=255) aCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAcgccg > 1:2299923/1‑41 (MQ=38) cTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGc < 1:3794814/71‑1 (MQ=255) tgtgCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCCGATGCGa > 1:2492461/1‑71 (MQ=255) tgCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACg < 1:4001086/71‑1 (MQ=255) | GCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACG > minE/337083‑337212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |