| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,804 | G→A | 14.5% | V338V (GTG→GTA) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,804 | 0 | G | A | 14.5% | 143.6 / 15.0 | 55 | V338V (GTG→GTA) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base G (26/21); new base A (4/4); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
ACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTG > minE/385739‑385872 | aCTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTgg < 1:1406235/71‑1 (MQ=255) gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCTGTGTCTGGCTTc < 1:2760385/71‑1 (MQ=255) gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCt < 1:914280/69‑1 (MQ=255) gTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgc > 1:3956390/1‑71 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa > 1:1956166/1‑66 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:1966939/1‑71 (MQ=255) gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg < 1:3891542/70‑1 (MQ=255) gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg < 1:3286345/70‑1 (MQ=255) gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGa > 1:3595653/1‑71 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt < 1:1313880/71‑1 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc > 1:1286010/1‑70 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:777853/1‑67 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct < 1:3176107/71‑1 (MQ=255) tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt < 1:2134460/71‑1 (MQ=255) aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc > 1:2480752/1‑51 (MQ=255) aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc > 1:3307805/1‑51 (MQ=255) aaCCTGTTCACGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTgg > 1:2256463/1‑50 (MQ=255) aCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttct > 1:3546339/1‑71 (MQ=255) aCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc < 1:1236067/70‑1 (MQ=255) cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtgt > 1:2883591/1‑43 (MQ=255) tGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa > 1:324983/1‑52 (MQ=255) tGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg > 1:1779544/1‑70 (MQ=255) tGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg > 1:1109884/1‑70 (MQ=255) tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCtcttcc < 1:2596108/68‑2 (MQ=255) gTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCggta > 1:3889583/1‑69 (MQ=255) ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt > 1:1065722/1‑42 (MQ=255) ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:3576218/1‑65 (MQ=255) cccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga > 1:3531833/1‑69 (MQ=255) cGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATctct > 1:3208517/1‑59 (MQ=255) gTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg < 1:1885310/52‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc < 1:2761013/35‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc < 1:1411244/35‑1 (MQ=255) ccTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:1577627/1‑70 (MQ=255) ccTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:3569403/1‑70 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgc < 1:3088359/41‑1 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAg < 1:3996159/69‑1 (MQ=255) aTCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCt < 1:483455/71‑1 (MQ=255) aTCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc < 1:439657/70‑1 (MQ=255) aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGa > 1:132746/1‑40 (MQ=255) cATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCt < 1:164617/66‑1 (MQ=255) tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat < 1:1062723/71‑2 (MQ=255) tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat < 1:2842094/71‑2 (MQ=255) tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat > 1:2736575/1‑70 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg > 1:3856479/1‑70 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:2907269/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:158737/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacaa < 1:2581713/71‑3 (MQ=255) cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:4100515/1‑39 (MQ=255) cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg > 1:77299/1‑71 (MQ=255) tgtgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:2566111/68‑1 (MQ=255) tgtgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAgg > 1:2097676/1‑71 (MQ=255) gtgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAcc > 1:742290/1‑44 (MQ=255) gtgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAcc > 1:3757692/1‑44 (MQ=255) ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAggg > 1:3465857/1‑69 (MQ=255) ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAggg > 1:2921285/1‑69 (MQ=255) gcgGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGaa < 1:743418/47‑1 (MQ=255) gtgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCg > 1:414362/1‑69 (MQ=255) gtgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGt > 1:4022824/1‑70 (MQ=255) gtgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTg > 1:829044/1‑71 (MQ=255) | ACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTG > minE/385739‑385872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |