| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,834 | G→T | 6.5% | G348G (GGG→GGT) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,834 | 0 | G | T | 6.5% | 253.2 / 2.5 | 76 | G348G (GGG→GGT) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base G (34/37); new base T (2/3); total (36/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
CCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAA > minE/385768‑385903 | cccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga > 1:3531833/1‑69 (MQ=255) ccTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:3569403/1‑70 (MQ=255) ccTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:1577627/1‑70 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAg < 1:3996159/69‑1 (MQ=255) aTCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCt < 1:483455/71‑1 (MQ=255) aTCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc < 1:439657/70‑1 (MQ=255) cATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCt < 1:164617/66‑1 (MQ=255) tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat > 1:2736575/1‑70 (MQ=255) tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat < 1:2842094/71‑2 (MQ=255) tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat < 1:1062723/71‑2 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:2907269/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:158737/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg > 1:3856479/1‑70 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacaa < 1:2581713/71‑3 (MQ=255) cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg > 1:77299/1‑71 (MQ=255) tgtgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:2566111/68‑1 (MQ=255) tgtgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAgg > 1:2097676/1‑71 (MQ=255) gtgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAcc > 1:3757692/1‑44 (MQ=255) gtgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAcc > 1:742290/1‑44 (MQ=255) ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAggg > 1:3465857/1‑69 (MQ=255) ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAggg > 1:2921285/1‑69 (MQ=255) gcgGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGaa < 1:743418/47‑1 (MQ=255) gtgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCg > 1:414362/1‑69 (MQ=255) gtgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGt > 1:4022824/1‑70 (MQ=255) gtgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTg > 1:829044/1‑71 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGaa < 1:2734030/41‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTgg > 1:345771/1‑49 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:3645840/56‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt < 1:2943414/71‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:54322/1‑71 (MQ=255) tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc > 1:3900882/1‑48 (MQ=255) tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa > 1:1125927/1‑71 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt < 1:1556019/68‑1 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt < 1:1100498/68‑1 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa < 1:547433/69‑1 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTAt > 1:2635685/1‑70 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATc > 1:3676676/1‑71 (MQ=255) cTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt < 1:3661634/67‑1 (MQ=255) cTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCg > 1:647106/1‑71 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGc > 1:4145850/1‑70 (MQ=255) cATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTa > 1:2520274/1‑71 (MQ=255) cATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTa < 1:3036159/71‑1 (MQ=255) cATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTa < 1:3602948/71‑1 (MQ=255) cATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTa < 1:1995040/71‑1 (MQ=255) tGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACg < 1:2020927/71‑1 (MQ=255) tGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACg < 1:2023850/71‑1 (MQ=255) cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg < 1:3569800/71‑1 (MQ=255) cTGATCTCTTCTGGGACCACGCCGAAGATGCTCGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg < 1:1793739/71‑1 (MQ=255) tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAc < 1:2580754/65‑1 (MQ=255) tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAc < 1:2372572/65‑1 (MQ=255) gATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa > 1:3324890/1‑71 (MQ=255) gATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa > 1:1037604/1‑71 (MQ=255) gATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa > 1:582615/1‑71 (MQ=255) gATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa > 1:1857763/1‑71 (MQ=255) aTCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:98470/37‑1 (MQ=255) aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATc < 1:686651/57‑1 (MQ=255) aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa < 1:1050874/70‑1 (MQ=255) aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa < 1:2580709/70‑1 (MQ=255) ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGt > 1:1168887/1‑64 (MQ=255) ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg < 1:332704/66‑1 (MQ=255) ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATg > 1:493619/1‑70 (MQ=255) ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt > 1:1090924/1‑71 (MQ=255) tcttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGc < 1:2250624/48‑1 (MQ=255) tcttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGc < 1:2158458/48‑1 (MQ=255) cttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAgg > 1:1055739/1‑42 (MQ=255) cttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt > 1:2907151/1‑70 (MQ=255) ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt < 1:3226865/48‑1 (MQ=255) ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt < 1:3231774/48‑1 (MQ=255) ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTATGGTGGGATGtt < 1:1360947/69‑1 (MQ=255) ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGAt < 1:794879/66‑1 (MQ=255) ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATg < 1:1417964/67‑1 (MQ=255) ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt < 1:502095/69‑1 (MQ=255) ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa > 1:1959911/1‑71 (MQ=255) ctGGGACCACGCCGAAGATGCTGGCGCACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGttta > 1:236578/1‑67 (MQ=255) ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAAt > 1:3802626/1‑70 (MQ=255) ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATg > 1:2662586/1‑71 (MQ=255) ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGaa < 1:184914/71‑1 (MQ=255) | CCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAA > minE/385768‑385903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |