| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 412,492 | C→T | 18.7% | intergenic (‑137/+152) | leuS ← / ← lnt | leucyl‑tRNA synthetase/apolipoprotein N‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 412,492 | 0 | C | T | 18.7% | 146.6 / 26.2 | 64 | intergenic (‑137/+152) | leuS/lnt | leucyl‑tRNA synthetase/apolipoprotein N‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (30/22); new base T (5/7); total (35/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
ATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTA > minE/412422‑412559 | atagcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCTCGCACTCGACGTTGAAATGATGCCCGGATTAc < 1:2388802/71‑1 (MQ=255) tagcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACt > 1:3453857/1‑71 (MQ=255) atagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATa < 1:1404499/71‑1 (MQ=255) gcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACAt > 1:898685/1‑71 (MQ=255) gcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACAt > 1:1677529/1‑71 (MQ=255) ccAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTc > 1:2829458/1‑63 (MQ=255) ccAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTc > 1:317797/1‑63 (MQ=255) aaaCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAAt > 1:2946677/1‑71 (MQ=255) aaaCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAAt > 1:1917871/1‑71 (MQ=255) aaCGTCCGCGTCAAAACAGCCTTTCTCGCACTCGACGTTGAAATGATG‑GCGGATTACTCa > 1:4021438/1‑60 (MQ=255) aaCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCa > 1:763870/1‑61 (MQ=255) aaCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAAtt < 1:529523/71‑1 (MQ=255) aCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTAATACATTAAtt > 1:2155204/1‑70 (MQ=255) cGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTa < 1:1618528/71‑1 (MQ=255) tCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTAc < 1:3053609/54‑1 (MQ=255) cgTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACagag < 1:136302/70‑1 (MQ=255) gTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGtt < 1:36780/71‑1 (MQ=255) tCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGttt < 1:3674367/71‑1 (MQ=255) aaaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACt > 1:711619/1‑47 (MQ=255) aaaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTc > 1:2268428/1‑48 (MQ=255) aaaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACag > 1:1495423/1‑64 (MQ=255) aaaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTtg < 1:648889/70‑1 (MQ=255) aaaCAGCCTTTCGCGCACTCGACGTTGATATGATGCCCGGATTACTCATACATTAATTTACAGAGTTtg < 1:1190062/69‑1 (MQ=255) aaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTtgtg > 1:1312853/1‑71 (MQ=255) aaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATa > 1:2850461/1‑49 (MQ=255) aaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATa > 1:1083890/1‑49 (MQ=255) ccTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTa < 1:3730663/71‑1 (MQ=255) cTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATTCATTAATTTACAGAGTTTGTGGGCGTAt < 1:2070649/71‑1 (MQ=255) cTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAAtt > 1:691051/1‑51 (MQ=255) tttCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTAt > 1:3837424/1‑70 (MQ=255) tttCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTAt > 1:3164205/1‑70 (MQ=255) tCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTtgtg > 1:3797935/1‑61 (MQ=255) tCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTag > 1:402850/1‑71 (MQ=255) gcACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGttt < 1:11162/54‑1 (MQ=255) tCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTAt < 1:1573833/60‑1 (MQ=255) cGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAg > 1:4042868/1‑71 (MQ=255) cGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAg > 1:2296633/1‑71 (MQ=255) gACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTtgtg > 1:1033498/1‑51 (MQ=255) gACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTtgtg > 1:1657858/1‑51 (MQ=255) gACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTtgt > 1:2403935/1‑50 (MQ=255) gACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAg > 1:253562/1‑70 (MQ=255) gTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGaa > 1:722316/1‑70 (MQ=255) gTTGAAATGATGCACGGATTACTCATACATTAATTTACAGAGttt > 1:327981/1‑45 (MQ=255) ttGAAATGATGCCCGGATTACTCATACATTAATTTACaga < 1:2524501/40‑1 (MQ=255) ttGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGttt < 1:1246278/44‑1 (MQ=255) gAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTtg > 1:947702/1‑43 (MQ=255) gAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACa > 1:4017472/1‑69 (MQ=255) aaaTGATGCCCGGATTATTCATACATTAATTTACag < 1:479715/36‑1 (MQ=255) aaaTGATGCCCGGATTATTCATACATTAATTTACag < 1:2701531/36‑1 (MQ=255) aaaTGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACaaa < 1:985994/70‑1 (MQ=255) aaaTGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACaaa < 1:2207209/70‑1 (MQ=255) aaaTGATGCCCGGATTACTCATACATTAATTTACAGAGTTtg < 1:3027604/42‑1 (MQ=255) aaTGATGCCCGGATTACTCATACATTAATTTACAGAGTTtg < 1:667239/41‑1 (MQ=255) aaTGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACaaa > 1:1268263/1‑69 (MQ=255) tgatgCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTagcaaagc < 1:972631/57‑1 (MQ=255) tgatgCCCGGATTACTCATACATTAATTTACAGAGTTtgtg > 1:2095050/1‑41 (MQ=255) gatgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACg > 1:1807049/1‑71 (MQ=255) atgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTagcaaagca < 1:1270661/56‑1 (MQ=255) tgCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTc < 1:1253275/71‑1 (MQ=255) tgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTc < 1:33949/71‑1 (MQ=255) cccGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAATAACGTCt < 1:4008617/70‑1 (MQ=255) ggATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCtatta > 1:1861141/1‑71 (MQ=255) ggATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAg < 1:3067001/52‑1 (MQ=255) aTTACTCATACATTAATTTACAGAGTTTGTGGGCg < 1:2426891/35‑1 (MQ=255) ttACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCtattatta < 1:712463/71‑1 (MQ=255) aCTCATACATTAATTTACAGAGTTTGTGGGCGTATTagca < 1:3681650/40‑1 (MQ=255) | ATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTA > minE/412422‑412559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |