| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 442,284 | G→A | 48.0% | P493L (CCA→CTA) | speF ← | ornithine decarboxylase isozyme, inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 442,284 | 0 | G | A | 48.0% | 11.2 / 144.4 | 100 | P493L (CCA→CTA) | speF | ornithine decarboxylase isozyme, inducible |
| Reads supporting (aligned to +/- strand): ref base G (27/25); new base A (21/27); total (48/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
TACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGG > minE/442217‑442352 | tACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACATGGGGTTGTCAGCAACAGTTTGCATGGAt > 1:1023830/1‑71 (MQ=255) cacCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATc > 1:1735089/1‑70 (MQ=255) cacCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATc > 1:3458637/1‑70 (MQ=255) acCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATgg < 1:1782622/66‑1 (MQ=255) ccGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATgg < 1:3247504/65‑1 (MQ=255) ccGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATgg < 1:1051623/65‑1 (MQ=255) ccGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATgg < 1:589859/65‑1 (MQ=255) cGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACa < 1:1741274/71‑1 (MQ=255) gAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGa > 1:797108/1‑64 (MQ=255) gAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGa > 1:3868195/1‑64 (MQ=255) gAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACaa < 1:3001491/71‑1 (MQ=255) aaCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACaa < 1:2151961/70‑1 (MQ=255) aCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAg < 1:3821715/71‑1 (MQ=255) aCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAg < 1:2606877/71‑1 (MQ=255) cATATTCGCCGTTACGTGCATCAATACCTGGGGTTTTCAGCAACAGTTTGCATAGATCGACAAAGTa < 1:2535895/67‑1 (MQ=255) cATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTAtt < 1:457797/69‑1 (MQ=255) atatTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGa < 1:3139390/70‑1 (MQ=255) atatTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGa < 1:4139131/70‑1 (MQ=255) atatTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGa < 1:2308571/70‑1 (MQ=255) tatTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGAt < 1:1786794/70‑1 (MQ=255) tatTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGa > 1:1603546/1‑54 (MQ=255) ttCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATtc > 1:1959646/1‑70 (MQ=255) ttCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTa > 1:1166360/1‑63 (MQ=255) ttCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTAtt < 1:1045554/65‑1 (MQ=255) ttCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATtct > 1:2123020/1‑71 (MQ=255) ttCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATtct > 1:2113235/1‑71 (MQ=255) tCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATtctc > 1:3355395/1‑71 (MQ=255) tCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATtctc > 1:417822/1‑71 (MQ=255) cGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTctct > 1:3238610/1‑71 (MQ=255) cGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCa < 1:1804806/71‑1 (MQ=255) ttACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTg < 1:3001273/67‑1 (MQ=255) aCGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGa > 1:2736587/1‑58 (MQ=255) aCGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGc > 1:4120998/1‑71 (MQ=255) aCGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACaaa > 1:734040/1‑51 (MQ=255) aCGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTAtt > 1:198435/1‑56 (MQ=255) aCGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTAtt > 1:709346/1‑56 (MQ=255) aCGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTAtt > 1:504198/1‑56 (MQ=255) aCGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAg < 1:793479/70‑1 (MQ=255) cGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGcc < 1:3440367/71‑1 (MQ=255) cGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGcc > 1:3183842/1‑71 (MQ=255) cGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGc < 1:1530662/70‑1 (MQ=255) gTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCt < 1:1550399/71‑1 (MQ=255) tGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCa < 1:1806326/64‑1 (MQ=255) cATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATa > 1:1425455/1‑64 (MQ=255) cATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGAt > 1:3309632/1‑39 (MQ=255) cATCAATACCTGGGGTTGTCAGCAACAGTTTGCATAGAt > 1:712203/1‑39 (MQ=255) tCAATACCTGGGGTTGTCAGCAACAGTTTGCATgg < 1:738009/35‑1 (MQ=255) tCAATACCTGGGGTTGTCAGCAACAGTTTGCATgg < 1:1153156/35‑1 (MQ=255) tCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCaaa < 1:346938/71‑1 (MQ=255) aaTACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACaaa > 1:4006197/1‑42 (MQ=255) aaTACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATa > 1:1248668/1‑60 (MQ=255) aaTACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCa > 1:1910876/1‑67 (MQ=255) aaTACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTa > 1:1175041/1‑45 (MQ=255) aaTACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATtct < 1:4016282/53‑1 (MQ=255) aaTACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCaa > 1:711542/1‑68 (MQ=255) aaTACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAg < 1:1353889/71‑1 (MQ=255) aaTACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAg < 1:2674750/71‑1 (MQ=255) aaTACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAg < 1:804958/71‑1 (MQ=255) aTACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAg > 1:1212003/1‑70 (MQ=255) tACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTg < 1:3164618/46‑1 (MQ=255) tACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATa < 1:512677/58‑1 (MQ=255) tACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATa < 1:965101/58‑1 (MQ=255) tACCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGc > 1:2472745/1‑60 (MQ=255) aCCTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAAt > 1:750987/1‑71 (MQ=255) ccTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAAt < 1:2370127/70‑1 (MQ=255) ccTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCa > 1:2614872/1‑54 (MQ=255) cTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGt < 1:3252579/39‑1 (MQ=255) cTGGGGTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGc < 1:1001659/71‑1 (MQ=255) ggggTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGc > 1:1653349/1‑69 (MQ=255) gggTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGcc < 1:576322/69‑1 (MQ=255) gggTTGTCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGcc < 1:1055412/69‑1 (MQ=255) ttGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCa < 1:1327066/47‑1 (MQ=255) ttGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCaa < 1:420899/57‑1 (MQ=255) ttGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATg < 1:3664833/64‑1 (MQ=255) ttGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATg < 1:2612303/64‑1 (MQ=255) gTCAGCAACAGTTTGCATGGATCGACAAAGTATTGAt > 1:2385461/1‑37 (MQ=255) gTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATa > 1:3938498/1‑47 (MQ=255) gTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGaa > 1:1768808/1‑60 (MQ=255) tCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATg < 1:1502988/61‑1 (MQ=255) tCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAg < 1:2969673/57‑1 (MQ=255) tCAGCAACAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTcc < 1:3590610/71‑1 (MQ=255) aGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGaa > 1:1483513/1‑57 (MQ=255) aGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTcccc > 1:3357160/1‑71 (MQ=255) aGCAACAGTTTGCATGGATCGACAAAGTATTGATTATCTGCATAGCCTTCAAAAGaa > 1:56127/1‑57 (MQ=255) cAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTg > 1:3169515/1‑71 (MQ=255) aCAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCaa < 1:2677714/48‑1 (MQ=255) aCAGTTTGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGc < 1:1749888/56‑1 (MQ=255) tttGCATGGATCGACAAAGTATTGATTCTCTGCATAGc < 1:2293483/38‑1 (MQ=255) tttGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACa > 1:822310/1‑70 (MQ=255) ttGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAg > 1:3803957/1‑54 (MQ=255) tGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTc > 1:2435833/1‑58 (MQ=255) tGCATAGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAAc > 1:3299530/1‑71 (MQ=255) gCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAAc < 1:2685407/70‑1 (MQ=255) gCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAAc < 1:3596375/70‑1 (MQ=255) cATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGaa > 1:23615/1‑45 (MQ=255) aTGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAgt < 1:1930063/51‑1 (MQ=255) aTGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTc < 1:1519808/55‑1 (MQ=255) aTGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTc < 1:1518202/55‑1 (MQ=255) aTGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTgg < 1:3959533/71‑1 (MQ=255) ggATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTccc > 1:3712129/1‑55 (MQ=255) | TACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGG > minE/442217‑442352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |