| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 454,989 | C→G | 10.4% | L32L (CTG→CTC) | ybgH ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 454,989 | 0 | C | G | 10.4% | 221.2 / 13.2 | 77 | L32L (CTG→CTC) | ybgH | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (33/36); new base G (4/4); total (37/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
CAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCG > minE/454921‑455058 | cAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTCGTTGATTGGTGAGATAGAGAATcag < 1:2849055/71‑1 (MQ=255)cAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag > 1:2636942/1‑71 (MQ=255)cAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag < 1:2689441/71‑1 (MQ=255)cAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag > 1:1569786/1‑71 (MQ=255)cAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag < 1:3778093/71‑1 (MQ=255) ggCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATca > 1:811000/1‑65 (MQ=255) cgcTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagca > 1:2224203/1‑66 (MQ=255) cTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag < 1:982885/62‑1 (MQ=255) cTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag < 1:2444174/62‑1 (MQ=255) cTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACg < 1:2228850/71‑1 (MQ=255) cTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACg < 1:61552/71‑1 (MQ=255) tAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag < 1:3327596/61‑1 (MQ=255) tAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag < 1:4038403/57‑1 (MQ=255) tAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGc < 1:1913923/71‑1 (MQ=255) tAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGc < 1:4097912/71‑1 (MQ=255) aaCTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGcc > 1:3240464/1‑71 (MQ=255) tCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag < 1:2466773/53‑1 (MQ=255) tCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCg > 1:3214852/1‑69 (MQ=255) tCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGt > 1:3580465/1‑70 (MQ=255) cGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTa > 1:2942984/1‑70 (MQ=255) gTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagca > 1:640421/1‑53 (MQ=255) gTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaa < 1:2377919/70‑1 (MQ=255) gTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTa < 1:2656889/69‑1 (MQ=255) gTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTa < 1:756171/69‑1 (MQ=255) gTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTa < 1:136861/69‑1 (MQ=255) ggCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGc > 1:3212056/1‑71 (MQ=255) gTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagca > 1:3908257/1‑47 (MQ=255) gTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagca > 1:1883470/1‑47 (MQ=255) tGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag < 1:3063919/44‑1 (MQ=255) tGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag < 1:1521846/44‑1 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagca > 1:3398756/1‑44 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATc > 1:1175394/1‑40 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACg < 1:1336543/51‑1 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACg < 1:3381634/51‑1 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACg < 1:4046149/51‑1 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa > 1:3014661/1‑70 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa < 1:700930/70‑1 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa < 1:1583992/70‑1 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa > 1:272347/1‑70 (MQ=255) aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAat < 1:704828/71‑1 (MQ=255) gTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAata > 1:1330484/1‑71 (MQ=255) gTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAata > 1:4131068/1‑71 (MQ=255) tATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACg < 1:1607978/49‑1 (MQ=255) tCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagca > 1:1014040/1‑40 (MQ=255) tCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATc > 1:671265/1‑36 (MQ=255) tCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACg < 1:1134709/47‑1 (MQ=255) tCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTc > 1:1368659/1‑71 (MQ=255) gTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagca > 1:3745011/1‑38 (MQ=255) gTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTcc > 1:2573458/1‑70 (MQ=255) ttGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACg < 1:55879/44‑1 (MQ=255) tATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGc < 1:3343843/38‑1 (MQ=255) aTTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAata > 1:2968897/1‑61 (MQ=255) aTTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAata > 1:3959543/1‑61 (MQ=255) ttttAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaa > 1:2994322/1‑57 (MQ=255) tttAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTg > 1:3699829/1‑71 (MQ=255) ttAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCa > 1:1472529/1‑39 (MQ=255) ttAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGc > 1:1468493/1‑51 (MQ=255) ttAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTg < 1:3326154/70‑1 (MQ=255) ttAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTg > 1:1661899/1‑70 (MQ=255) ttAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTg < 1:4121510/70‑1 (MQ=255) tAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTg > 1:1307684/1‑69 (MQ=255) gTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTATAAGCTAAAata < 1:3876936/55‑1 (MQ=255) gATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAata > 1:960131/1‑52 (MQ=255) gATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgcgc > 1:2511377/1‑70 (MQ=255) ttGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTa < 1:2313457/38‑1 (MQ=255) ttGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAAc < 1:1794726/71‑1 (MQ=255) ggTGAGATAGAGAATGAGTAAGGCACGCATGCCGTaa > 1:3891473/1‑37 (MQ=37) gTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGa < 1:1915790/70‑1 (MQ=255) tGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGa < 1:2368093/54‑1 (MQ=255) tGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGAc > 1:1052665/1‑70 (MQ=255) tGAGATAGAGAATCAG‑AGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGa < 1:206121/53‑1 (MQ=255) agaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGata > 1:3795297/1‑67 (MQ=255) agaTAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCATCGACa < 1:819345/69‑1 (MQ=255) gaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGAtata < 1:3559243/70‑1 (MQ=255) aTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACtg < 1:775266/37‑2 (MQ=19) aTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACtg < 1:1679394/37‑2 (MQ=19) aTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACtg < 1:3218011/37‑2 (MQ=19) aTAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAg > 1:1310295/1‑70 (MQ=255) aTAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGt < 1:1541908/71‑1 (MQ=255) tAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTcc < 1:2200551/45‑1 (MQ=255) tAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTa < 1:3613702/71‑1 (MQ=255) tAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTa < 1:9308/71‑1 (MQ=255) tAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTa < 1:83469/71‑1 (MQ=255) agagAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGAtcgcca > 1:352824/1‑58 (MQ=255) agagAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAat > 1:2821213/1‑39 (MQ=255) agagAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgc > 1:1399834/1‑56 (MQ=255) agagAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTa > 1:2988942/1‑70 (MQ=255) gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGc > 1:3460408/1‑52 (MQ=255) agaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa > 1:581079/1‑70 (MQ=255) agaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa > 1:708108/1‑70 (MQ=255) gaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTatatc > 1:3956993/1‑70 (MQ=255) gaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACAt > 1:822525/1‑63 (MQ=255) aaTCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGAc > 1:1784888/1‑60 (MQ=255) aTCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAg < 1:1540939/70‑1 (MQ=255) tCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTc < 1:315200/37‑1 (MQ=255) tCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTc < 1:3549167/37‑1 (MQ=255) tCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTGCCAGATTTGCAGCGCAACGACATAGTAAATAgcg > 1:1085761/1‑71 (MQ=255) tCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAAt < 1:3237565/67‑1 (MQ=255) tCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcg > 1:72838/1‑71 (MQ=255) | CAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCG > minE/454921‑455058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |