| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 473,506 | G→A | 53.1% | K356K (AAG→AAA) | mngA → | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 473,506 | 0 | G | A | 53.1% | ‑0.0 / 69.1 | 49 | K356K (AAG→AAA) | mngA | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components |
| Reads supporting (aligned to +/- strand): ref base G (12/11); new base A (17/9); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01 | |||||||||||
TCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGG > minE/473441‑473573 | tCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTg < 1:3301730/69‑1 (MQ=255) aTCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGcggcgg > 1:1254100/1‑71 (MQ=255) aTCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGcggcgg > 1:3782419/1‑71 (MQ=255) cTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGgc > 1:3474064/1‑71 (MQ=255) cGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCggg < 1:3238711/67‑1 (MQ=255) cGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGgc < 1:2132885/68‑1 (MQ=255) cTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCgg < 1:2384453/71‑1 (MQ=255) cTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCgg < 1:1083786/71‑1 (MQ=255) cAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAAt > 1:1648425/1‑71 (MQ=255) aGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGggcggc > 1:2245640/1‑55 (MQ=255) gATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAAtttt < 1:764585/71‑1 (MQ=255) cTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGAt > 1:1403180/1‑71 (MQ=255) tttAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCt > 1:3618411/1‑41 (MQ=255) tttAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAAtttt > 1:3460979/1‑65 (MQ=255) tttAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAAtttt > 1:480020/1‑65 (MQ=255) tttAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGAt < 1:3978929/68‑1 (MQ=255) tttAATGAAGAAAACTCCTGGC‑‑TGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGAt < 1:3372004/66‑1 (MQ=255) tAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGAt > 1:2660182/1‑66 (MQ=255) tAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGggcggc > 1:1631794/1‑46 (MQ=255) aaaTGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGgctgc > 1:2715238/2‑53 (MQ=255) aTGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTAcc < 1:2582037/70‑1 (MQ=255) tGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGgctg > 1:1526277/1‑49 (MQ=255) tGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGgctg > 1:1531125/1‑49 (MQ=255) tGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAAtttt < 1:3216055/60‑1 (MQ=255) gaagaaAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGAt < 1:1899664/62‑1 (MQ=255) gaaAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGa > 1:3438981/1‑58 (MQ=255) gaaAACTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTg > 1:803846/1‑57 (MQ=255) aaaaCTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAAtt < 1:918132/53‑1 (MQ=255) aaaaCTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAAtt < 1:2657710/53‑1 (MQ=255) aaCTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGaa > 1:3382522/1‑49 (MQ=255) aaCTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGaa > 1:798374/1‑49 (MQ=255) cTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATg > 1:1319474/1‑55 (MQ=255) cTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCgg < 1:3028821/71‑1 (MQ=255) cTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCgg < 1:2732039/71‑1 (MQ=255) cTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTg > 1:739767/1‑52 (MQ=255) cTCCTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCgg > 1:1849790/1‑71 (MQ=255) cTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGcc < 1:431439/70‑1 (MQ=255) cTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGcc < 1:3381861/70‑1 (MQ=255) cTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGcc < 1:2919703/70‑1 (MQ=255) cTGGCTGTGGATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGcc > 1:269703/1‑70 (MQ=255) tgGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAAttt < 1:688319/40‑1 (MQ=255) tgGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTAcc < 1:2460106/50‑1 (MQ=255) gATGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGcc > 1:3151170/1‑70 (MQ=255) tGTACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGATgg > 1:844027/1‑42 (MQ=255) tACCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGa > 1:2463525/1‑37 (MQ=255) aCCGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATtct > 1:3616388/1‑71 (MQ=255) ccGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATtctc > 1:3812150/1‑71 (MQ=255) ccGCAAACTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATtctc > 1:3262884/1‑71 (MQ=255) cAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTgg > 1:2787645/1‑71 (MQ=255) | TCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGG > minE/473441‑473573 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |