| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 505,424 | T→C | 8.3% | G128G (GGT→GGC) | ybhE → | 6‑phosphogluconolactonase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 505,424 | 0 | T | C | 8.3% | 159.5 / 6.5 | 60 | G128G (GGT→GGC) | ybhE | 6‑phosphogluconolactonase |
| Reads supporting (aligned to +/- strand): ref base T (24/31); new base C (2/3); total (26/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01 | |||||||||||
TGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTT > minE/505357‑505494 | tGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGc < 1:1186661/71‑1 (MQ=255) gAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGc > 1:91908/1‑70 (MQ=255) gAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGc > 1:31801/1‑70 (MQ=255) aGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGTGGCTGGACGGTTGCCa > 1:4034089/1‑71 (MQ=255) aGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGcc < 1:902417/70‑1 (MQ=255) aGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCa > 1:3219566/1‑71 (MQ=255) aGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCa < 1:134789/71‑1 (MQ=255) gCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTTGACGGCTGCCAt < 1:2310320/71‑1 (MQ=255) gCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCAt < 1:2866200/71‑1 (MQ=255) cGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCAt < 1:1932866/70‑1 (MQ=255) tAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTcc > 1:4134700/1‑71 (MQ=255) tAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTcc > 1:2707845/1‑71 (MQ=255) cgcgTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGcc < 1:1088997/71‑1 (MQ=255) cgcgTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGcc < 1:1415101/71‑1 (MQ=255) tCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGtt < 1:1745232/56‑1 (MQ=255) tCTGGAAGATGGCCTGCCAGTGGGCGTCGGCGATGTGGGCGAGGGGCTGGACGGTTGc < 1:3700325/58‑1 (MQ=255) tGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATAtc > 1:495321/1‑71 (MQ=255) gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATAtct < 1:3935198/70‑1 (MQ=255) gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATAtct < 1:1343170/70‑1 (MQ=255) gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATAtct > 1:2935586/1‑70 (MQ=255) aTGGCCTGCCAGTGGGCGTCGTCGATGTGTTCGAGGTGCTGGACGGTTGCCATTCCGCCAATATCTCACCg > 1:1371080/1‑71 (MQ=255) gCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGa > 1:3304726/1‑70 (MQ=255) gCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGa > 1:1121240/1‑70 (MQ=255) gCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGa > 1:939776/1‑70 (MQ=255) ccAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCg < 1:1731934/71‑1 (MQ=255) gTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTAc < 1:919116/71‑1 (MQ=255) tGGGCGGCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTAc < 1:1032755/70‑1 (MQ=255) ggCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGc < 1:2436825/70‑1 (MQ=255) gCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtg < 1:3844535/71‑1 (MQ=255) gCGTCGTCGATGTGGGCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtg < 1:1855220/71‑1 (MQ=255) cgtcgtCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtgt < 1:3590873/71‑1 (MQ=255) gtcgtcGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtgtg < 1:2463787/71‑1 (MQ=255) gtcgtcGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtgtg < 1:3814532/71‑1 (MQ=255) tcgtcgATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATAtctc < 1:2035392/49‑1 (MQ=255) gtcgATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAata > 1:3710745/1‑43 (MQ=255) gtcgATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATAtc > 1:789500/1‑45 (MQ=255) gtcgATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGt < 1:1020992/71‑1 (MQ=255) gtcgATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGt > 1:801258/1‑71 (MQ=255) gtcgATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGt > 1:4056240/1‑71 (MQ=255) cgATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTggggtt > 1:1233959/1‑68 (MQ=255) gATGTGGTCTAGGGGCTGGACGGTTGCCATTCCGCCaa > 1:991752/1‑38 (MQ=255) gATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCaa > 1:2506401/1‑38 (MQ=255) gATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtgt > 1:2696492/1‑64 (MQ=255) tgtgGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCgg < 1:1922203/71‑1 (MQ=255) gtgGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCgg > 1:1325666/1‑70 (MQ=255) tgGTCGAGGGGCTGGACGGTTGCCATTCCGCGAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCa < 1:3330334/71‑1 (MQ=255) tgGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCa < 1:3843048/71‑1 (MQ=255) gTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTa > 1:2491241/1‑52 (MQ=255) gTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTa > 1:2365700/1‑52 (MQ=255) gTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCAtt < 1:1819138/71‑1 (MQ=255) gTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCAtt < 1:698910/71‑1 (MQ=255) gTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCAt < 1:2595541/70‑1 (MQ=255) gTCGAGGGGCTGGACGGTTGCCATTACGCCAATATCTCACCGGACAACCGTa > 1:3527613/1‑52 (MQ=255) gggCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGc < 1:1815065/70‑1 (MQ=255) ggCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAg < 1:3637621/71‑1 (MQ=255) tGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTa < 1:948061/62‑1 (MQ=255) gACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGAtgg > 1:1218677/1‑69 (MQ=255) gACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCg < 1:3081932/71‑1 (MQ=255) gACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCg > 1:3392173/1‑71 (MQ=255) cGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCa < 1:1743799/71‑1 (MQ=255) cGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCa < 1:2396405/71‑1 (MQ=255) ttGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCAttt < 1:2464217/71‑1 (MQ=255) | TGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTT > minE/505357‑505494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |