| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 509,821 | C→T | 9.1% | intergenic (+46/‑137) | ybhI → / → ybhJ | predicted transporter/predicted hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 509,821 | 0 | C | T | 9.1% | 169.9 / 6.1 | 55 | intergenic (+46/‑137) | ybhI/ybhJ | predicted transporter/predicted hydratase |
| Reads supporting (aligned to +/- strand): ref base C (26/24); new base T (2/3); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATT > minE/509752‑509891 | cTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt < 1:3713231/71‑1 (MQ=255) tGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt > 1:2322320/1‑70 (MQ=255) tGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt > 1:3057471/1‑70 (MQ=255) tGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt > 1:783804/1‑70 (MQ=255) gATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCa > 1:1117018/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:4085570/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:3180230/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:3527853/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:1088306/1‑71 (MQ=255) ggCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc < 1:571318/70‑1 (MQ=255) ggCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:1725350/1‑70 (MQ=255) ggCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:1045325/1‑70 (MQ=255) ggCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcc < 1:694862/69‑1 (MQ=255) gCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:2873067/1‑69 (MQ=255) gCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:282098/1‑69 (MQ=255) ggAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt > 1:2699810/1‑70 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:1041318/69‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTc < 1:1411236/70‑1 (MQ=255) aaCATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa < 1:942315/70‑1 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcccc < 1:66095/62‑1 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcccc < 1:1474017/62‑1 (MQ=255) tGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcccc < 1:515978/61‑1 (MQ=255) tGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttac > 1:1219257/1‑71 (MQ=255) tGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttac > 1:1904882/1‑71 (MQ=255) cTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttactt < 1:3319265/71‑1 (MQ=255) cTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttactt < 1:3536703/71‑1 (MQ=255) cTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttact > 1:4086443/1‑70 (MQ=255) cTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttact > 1:782243/1‑70 (MQ=255) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt > 1:455418/1‑70 (MQ=39) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt < 1:1756737/70‑1 (MQ=39) tatTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTAACTTACTTATGTa < 1:1111222/69‑1 (MQ=25) ttCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa > 1:1728327/1‑55 (MQ=33) ttCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc < 1:3753678/70‑1 (MQ=33) ttCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCt < 1:2882048/71‑1 (MQ=32) tctcGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCt < 1:173473/70‑1 (MQ=31) gCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCtt < 1:1531962/70‑1 (MQ=11) cATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTg < 1:3442222/70‑1 (MQ=35) tgtaCTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCg > 1:3799583/1‑71 (MQ=11) tgctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGc > 1:590296/1‑70 (MQ=35) tgctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGc < 1:924492/70‑1 (MQ=35) ctgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCtt > 1:2569819/1‑71 (MQ=34) tgcgactgcgtTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCg < 1:2931044/59‑1 (MQ=11) gcgactgcgtTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGt > 1:3854158/1‑63 (MQ=11) cgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTcc < 1:2220993/70‑1 (MQ=35) gtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTg > 1:2208277/1‑71 (MQ=34) tctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGc < 1:2744968/71‑1 (MQ=34) actgcgtTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGc < 1:1384139/70‑1 (MQ=255) gcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCaa > 1:3056655/1‑70 (MQ=35) cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCaa > 1:796985/1‑69 (MQ=35) cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCaa < 1:253782/69‑1 (MQ=35) cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAAc < 1:513398/70‑1 (MQ=35) cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACt > 1:1684521/1‑71 (MQ=34) tCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGCTTGTCGCCTTCCTGCAACTCg > 1:4031425/1‑70 (MQ=25) gCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGaa < 1:2833512/70‑1 (MQ=35) gCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAat < 1:641259/71‑1 (MQ=34) cTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAatt < 1:3788106/71‑1 (MQ=34) | CTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATT > minE/509752‑509891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |