| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 518,289 | G→A | 18.6% | D105N (GAT→AAT) | bioC → | predicted methltransferase, enzyme of biotin synthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 518,289 | 0 | G | A | 18.6% | 159.9 / 28.3 | 70 | D105N (GAT→AAT) | bioC | predicted methltransferase, enzyme of biotin synthesis |
| Reads supporting (aligned to +/- strand): ref base G (34/23); new base A (6/7); total (40/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCG > minE/518220‑518359 | cAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGa < 1:3230205/71‑1 (MQ=255) gAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGAt > 1:2408037/1‑70 (MQ=255) gAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGAt > 1:1423549/1‑70 (MQ=255) aGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCtt > 1:4138256/1‑71 (MQ=255) gATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTg < 1:2389395/70‑1 (MQ=255) cGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTA‑‑GACTGCGACGTTCGATCTTGCATGGAg > 1:1944329/1‑70 (MQ=255) cAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGc < 1:561804/71‑1 (MQ=255) cAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGc < 1:4014692/71‑1 (MQ=255) cAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGc < 1:2662252/64‑1 (MQ=255) aggccATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCa < 1:451400/68‑1 (MQ=255) aGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCa < 1:1449823/71‑1 (MQ=255) aCCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCCACTGCGACGTTCg < 1:1699943/54‑1 (MQ=255) aCCATTATCTGGCGGGAGATATAGAATCCCTGCCGTTAGCGACTGCGACGTTCGATc > 1:815493/1‑57 (MQ=255) ccATAATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAAtc > 1:2811345/1‑71 (MQ=255) cATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCa > 1:3533218/1‑60 (MQ=255) ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATg < 1:1141432/60‑1 (MQ=255) ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAAtct > 1:1536406/1‑69 (MQ=255) ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCg < 1:2634982/71‑1 (MQ=255) ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCg > 1:2007936/1‑71 (MQ=255) ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCg > 1:345076/1‑71 (MQ=255) ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCg < 1:69013/71‑1 (MQ=255) ggCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCg > 1:367088/1‑44 (MQ=255) gCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGc > 1:1968469/1‑50 (MQ=255) gCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgcagtg < 1:3434001/69‑1 (MQ=255) gggAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgcagtgcagt < 1:455413/71‑1 (MQ=255) ggAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgca > 1:492385/1‑63 (MQ=255) gaTATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGAt > 1:2225484/1‑39 (MQ=255) atatCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgcagtgcag < 1:2153417/65‑1 (MQ=255) atatCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgcagtgcag < 1:2269026/65‑1 (MQ=255) atCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCa > 1:2889542/1‑42 (MQ=255) atCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCa > 1:2743168/1‑42 (MQ=255) atCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCa > 1:1313528/1‑42 (MQ=255) atCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg > 1:1612664/1‑71 (MQ=255) tCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAAt > 1:1079925/1‑50 (MQ=255) tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgcagtgcag < 1:2022379/62‑1 (MQ=255) gAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGa > 1:742500/1‑43 (MQ=255) gAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGa > 1:4130244/1‑43 (MQ=255) gAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCg < 1:3956721/52‑1 (MQ=255) gAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCg < 1:676633/52‑1 (MQ=255) gAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTaa > 1:2192894/1‑71 (MQ=255) gAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTaa > 1:1573534/1‑71 (MQ=255) aTCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt < 1:3709868/71‑1 (MQ=255) aTCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt > 1:2878295/1‑71 (MQ=255) tCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAttt < 1:3253522/71‑1 (MQ=255) tCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt > 1:3385211/1‑70 (MQ=255) tCCCTGCCGTTAGCGACTGCGACGTTCGATCTT‑CATGGa > 1:1576807/1‑39 (MQ=39) tGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAttt < 1:1907932/67‑1 (MQ=255) gCCGTTAGCGACTGCGACGTTCGATCTTGCATGGGGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa > 1:3357977/1‑71 (MQ=255) gCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa > 1:3271016/1‑71 (MQ=255) gCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa > 1:1263228/1‑71 (MQ=255) ccGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt > 1:1128314/1‑64 (MQ=255) cGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTa > 1:3620925/1‑60 (MQ=255) ttAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgcagtg < 1:1594129/45‑1 (MQ=255) ttAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACgg > 1:338412/1‑70 (MQ=255) ttAGCGACTGCGACGTTCAATCTTGCATTGAGCAATCTCgca > 1:2055005/1‑42 (MQ=255) ttAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc > 1:122175/1‑65 (MQ=255) ttAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACgg > 1:1589864/1‑70 (MQ=255) aGCGACTGCGACGTTCGATCTTGCATGGAGCAAtct > 1:2385726/1‑36 (MQ=255) aGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCAc > 1:389640/1‑71 (MQ=255) aGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCAc > 1:3125618/1‑71 (MQ=255) cGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTc < 1:248502/71‑1 (MQ=255) cGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTc < 1:2560008/71‑1 (MQ=255) aCTGCGACGTTCGATCTTGCATGGAGCAATCTCgcag > 1:273611/1‑37 (MQ=255) tGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc < 1:3699364/57‑1 (MQ=255) gCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTaa > 1:86602/1‑50 (MQ=255) gACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAttt < 1:2738943/51‑1 (MQ=255) gACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc < 1:3555310/54‑1 (MQ=255) cGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTAt > 1:847082/1‑51 (MQ=255) gTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGt < 1:634729/71‑1 (MQ=255) ttCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTa < 1:3837582/48‑1 (MQ=255) ttCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCTGCGAGCTGTa < 1:1089957/71‑1 (MQ=255) ttCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc < 1:2770073/50‑1 (MQ=255) gATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCg > 1:546575/1‑71 (MQ=255) | CAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCG > minE/518220‑518359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |