Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,090	T→G	9.8%	N722H (AAC→CAC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,090	0	T	G	9.8%	146.4 / 13.7	61	N722H (AAC→CAC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (24/31);  new base G (3/3);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

AGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATG  >  minE/608022‑608157                                                                     |                                                                    aGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGttt                                                                    <  1:1027952/70‑1 (MQ=255)  gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc                                                                  <  1:1618702/71‑1 (MQ=255)     aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt                                                               >  1:2956635/1‑71 (MQ=255)      gCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                                                                     <  1:3576736/64‑1 (MQ=255)         aGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcgcg                                                           <  1:2970336/71‑1 (MQ=255)             gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTc                                                         >  1:3850627/1‑69 (MQ=255)                aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                                                      >  1:255551/1‑69 (MQ=255)                   tACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc                                                                 >  1:2499353/1‑55 (MQ=255)                   tACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc                                                                 >  1:2191725/1‑55 (MQ=255)                     cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                               >  1:833715/1‑71 (MQ=255)                     cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                               <  1:2941602/71‑1 (MQ=255)                     cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                               >  1:3755243/1‑71 (MQ=255)                       gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                               >  1:789006/1‑69 (MQ=255)                       gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                               >  1:1781679/1‑69 (MQ=255)                          tAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGttt                                                                    <  1:658584/45‑1 (MQ=255)                            gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                         <  1:2782407/70‑1 (MQ=255)                            gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                         <  1:3759950/70‑1 (MQ=255)                            gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                         <  1:4059636/70‑1 (MQ=255)                            gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                         <  1:1554197/70‑1 (MQ=255)                            gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt                                        >  1:2263107/1‑71 (MQ=255)                             ccAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                       >  1:678018/1‑71 (MQ=255)                                gATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc                                                                  <  1:439395/41‑1 (MQ=255)                                  tACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                                                                     >  1:1817748/1‑36 (MQ=40)                                  tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                                                      >  1:2113819/1‑51 (MQ=255)                                  tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                           >  1:2117928/1‑62 (MQ=255)                                  tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatca                                  >  1:2134546/1‑71 (MQ=255)                                    cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                          <  1:3307487/61‑1 (MQ=255)                                    cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                 <  1:954018/70‑1 (MQ=255)                                      cGGATGGTCAGCT‑‑GGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt                                        >  1:3782096/1‑59 (MQ=38)                                       ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa                             <  1:2862875/71‑1 (MQ=255)                                       ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa                             <  1:2867888/71‑1 (MQ=255)                                        gATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGttga                            <  1:2025127/71‑3 (MQ=255)                                        gATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                            <  1:3732417/71‑1 (MQ=255)                                            gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                   >  1:2968005/1‑60 (MQ=255)                                            gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                        <  1:1895377/71‑1 (MQ=255)                                             tCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                       <  1:2209672/71‑1 (MQ=255)                                              cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                        >  1:341219/1‑69 (MQ=255)                                               aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                         >  1:873134/1‑51 (MQ=255)                                               aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                      <  1:428259/70‑1 (MQ=255)                                                   taggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAttt                                              >  1:1509493/3‑42 (MQ=12)                                                   taggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt                 >  1:2042885/3‑70 (MQ=255)                                                    cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                    <  1:3068170/51‑1 (MQ=255)                                                    cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACg                          <  1:1592085/61‑1 (MQ=255)                                                    cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                <  1:2993827/71‑1 (MQ=255)                                                    aggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgtt                >  1:1240154/2‑69 (MQ=255)                                                     ggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTg               <  1:808871/71‑1 (MQ=12)                                                      gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                       <  1:3237633/46‑1 (MQ=255)                                                      gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa              >  1:87146/1‑71 (MQ=255)                                                      gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAtt                                       <  1:2344523/46‑1 (MQ=12)                                                       atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                        <  1:2751272/60‑1 (MQ=255)                                                       atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGac             >  1:2458871/1‑71 (MQ=255)                                                          tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                    <  1:2823193/45‑1 (MQ=255)                                                            tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                      >  1:1845417/1‑57 (MQ=255)                                                            tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                >  1:3168975/1‑63 (MQ=255)                                                            tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc         >  1:747842/1‑70 (MQ=255)                                                               ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc      <  1:1257222/70‑1 (MQ=255)                                                               ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc      <  1:2698739/70‑1 (MQ=255)                                                                cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                  <  1:2694289/57‑1 (MQ=255)                                                                cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacc            <  1:1602284/63‑1 (MQ=255)                                                                  ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg  <  1:662534/71‑1 (MQ=255)                                                                  ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg  <  1:2205812/71‑1 (MQ=255)                                                                  ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg  <  1:757415/71‑1 (MQ=255)                                                                   gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                        <  1:3973057/48‑1 (MQ=255)                                                                   gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                        <  1:178005/48‑1 (MQ=255)                                                                   gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg  >  1:1728911/1‑70 (MQ=255)                                                                    ttttCCATCGCGTCGAGCATCATTTCACGGTTCATCACGTTAACGtt                        <  1:3039242/47‑1 (MQ=255)                                                                    ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                       <  1:2731694/48‑1 (MQ=255)                                                                     tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                 <  1:1306678/37‑1 (MQ=255)                                                                     tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                 <  1:2926582/37‑1 (MQ=255)                                                                     tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                 <  1:2931425/37‑1 (MQ=255)                                                                     |                                                                    AGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATG  >  minE/608022‑608157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑