| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,203 | T→G | 7.4% | S234A (TCC→GCC) ‡ | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,203 | 0 | T | G | 7.4% | 171.6 / 9.9 | 68 | S234A (TCC→GCC) ‡ | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (30/33); new base G (2/3); total (32/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGT > minE/189141‑189273 | gTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCAt > 1:706291/1‑63 (MQ=255)gTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCAt > 1:767364/1‑63 (MQ=255)gTTATATCGTTACCAACGGAACATCGACGTCGAACAAAAT‑‑TGGGTATGTACGCCGCGCCAt > 1:2869515/1‑61 (MQ=255) atatCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACg > 1:3513510/1‑71 (MQ=255) cGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGt > 1:653708/1‑71 (MQ=255) cGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGt > 1:2555742/1‑71 (MQ=255) ccAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc < 1:1946526/71‑1 (MQ=255) ccAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc < 1:2727966/71‑1 (MQ=255) cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:164692/71‑1 (MQ=255) gAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCg > 1:1014092/1‑70 (MQ=255) aaCATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGc > 1:1378356/1‑70 (MQ=255) aTCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTg < 1:1098583/58‑1 (MQ=255) tCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCt > 1:3628666/1‑53 (MQ=255) tCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGa < 1:1796754/58‑1 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATc > 1:1086150/1‑37 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCaa > 1:1885878/1‑64 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAt > 1:3197765/1‑65 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAt > 1:797214/1‑65 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCa > 1:3904913/1‑70 (MQ=255) gTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCAt > 1:3034066/1‑70 (MQ=255) tCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa < 1:2037503/40‑1 (MQ=255) tCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaa < 1:2770815/71‑1 (MQ=255) gcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTaac > 1:2405916/2‑62 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCaca < 1:273590/69‑3 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCaca < 1:3512941/69‑3 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaaa > 1:1401832/1‑71 (MQ=255) cGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa > 1:3220448/1‑39 (MQ=255) cGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaaa > 1:3138174/1‑71 (MQ=255) gAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACAAAt < 1:2307331/71‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGt > 1:2458660/1‑40 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTa > 1:2325206/1‑41 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc < 1:2889164/52‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:3339934/1‑71 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:1477894/1‑71 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:1496904/1‑71 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:1743851/1‑71 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt < 1:3273680/70‑1 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:496206/71‑1 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3982843/71‑1 (MQ=255) aaaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:3631015/48‑1 (MQ=255) aTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTgg < 1:1411818/70‑1 (MQ=255) aTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGgc < 1:2569125/71‑1 (MQ=255) aTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGgc > 1:2122675/1‑71 (MQ=255) tgGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCAt > 1:1077841/1‑71 (MQ=255) tATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTc < 1:1746820/50‑1 (MQ=255) tATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATa < 1:1588600/53‑1 (MQ=255) tATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTg > 1:457434/1‑70 (MQ=255) aTGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaaa > 1:2951659/1‑54 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGc < 1:2170685/57‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:2885797/70‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:657538/70‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:3136098/70‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt > 1:2830645/1‑70 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:2088505/70‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:1432597/70‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtg < 1:2062267/71‑1 (MQ=255) tGTACGCCGCGCAATCCGGCAGTACGCTGTTGATc < 1:2547372/35‑1 (MQ=255) tACGCCGCGCCATCCGGCAGTACGCTGTTGATCGa > 1:621255/1‑35 (MQ=255) tACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat < 1:844132/71‑1 (MQ=255) tACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat < 1:275260/71‑1 (MQ=255) tACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat < 1:2737414/71‑1 (MQ=255) tACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGAAATTGTCATAAATCGCTGGCGCATCTGTtgat > 1:840124/1‑71 (MQ=255) cgccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatg < 1:13200/70‑1 (MQ=255) cgccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatg < 1:1356474/70‑1 (MQ=255) cgccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatg < 1:18112/70‑1 (MQ=255) cgcgCCATCCGGCAGTACGCTGTTGATCGCCCGCAATTGTCATAAATCGCTGGCGCAt > 1:3565757/1‑58 (MQ=255) cgcgCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtga > 1:3408992/1‑65 (MQ=255) gcCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaa < 1:1910474/42‑1 (MQ=255) ccATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTg < 1:2507119/57‑1 (MQ=255) cATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGa > 1:376090/1‑70 (MQ=255) aTCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATg < 1:1523124/71‑1 (MQ=255) tCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATg > 1:2763529/1‑70 (MQ=255) tCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATg < 1:3182782/70‑1 (MQ=255) tCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGt < 1:503471/71‑1 (MQ=255) tCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGt < 1:2262521/71‑1 (MQ=255) tCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGt < 1:446770/71‑1 (MQ=255) | GTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGT > minE/189141‑189273 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |