Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,097	C→G	8.9%	A719A (GCG→GCC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,097	0	C	G	8.9%	197.8 / 8.1	67	A719A (GCG→GCC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (26/35);  new base G (3/3);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

AGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCT  >  minE/608030‑608163                                                                    |                                                                   aGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcgcg                                                                 <  1:2970336/71‑1 (MQ=255)     gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTc                                                               >  1:3850627/1‑69 (MQ=255)        aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                                                            >  1:255551/1‑69 (MQ=255)             cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                     <  1:2941602/71‑1 (MQ=255)             cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                     >  1:3755243/1‑71 (MQ=255)             cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                     >  1:833715/1‑71 (MQ=255)               gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                     >  1:789006/1‑69 (MQ=255)               gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                     >  1:1781679/1‑69 (MQ=255)                    gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                               <  1:2782407/70‑1 (MQ=255)                    gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                               <  1:1554197/70‑1 (MQ=255)                    gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                               <  1:4059636/70‑1 (MQ=255)                    gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                               <  1:3759950/70‑1 (MQ=255)                    gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt                                              >  1:2263107/1‑71 (MQ=255)                     ccAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                             >  1:678018/1‑71 (MQ=255)                          tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                                                            >  1:2113819/1‑51 (MQ=255)                          tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                                 >  1:2117928/1‑62 (MQ=255)                          tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatca                                        >  1:2134546/1‑71 (MQ=255)                            cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                                <  1:3307487/61‑1 (MQ=255)                            cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                       <  1:954018/70‑1 (MQ=255)                              cGGATGGTCAGCT‑‑GGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt                                              >  1:3782096/1‑59 (MQ=38)                               ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa                                   <  1:2867888/71‑1 (MQ=255)                               ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa                                   <  1:2862875/71‑1 (MQ=255)                                gATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGttga                                  <  1:2025127/71‑3 (MQ=255)                                gATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                  <  1:3732417/71‑1 (MQ=255)                                    gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                         >  1:2968005/1‑60 (MQ=255)                                    gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                              <  1:1895377/71‑1 (MQ=255)                                     tCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                             <  1:2209672/71‑1 (MQ=255)                                      cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                              >  1:341219/1‑69 (MQ=255)                                       aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                               >  1:873134/1‑51 (MQ=255)                                       aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                            <  1:428259/70‑1 (MQ=255)                                           taggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAttt                                                    >  1:1509493/3‑42 (MQ=12)                                           taggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt                       >  1:2042885/3‑70 (MQ=255)                                            cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                          <  1:3068170/51‑1 (MQ=255)                                            cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACg                                <  1:1592085/61‑1 (MQ=255)                                            cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                      <  1:2993827/71‑1 (MQ=255)                                            aggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgtt                      >  1:1240154/2‑69 (MQ=255)                                             ggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTg                     <  1:808871/71‑1 (MQ=12)                                              gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                             <  1:3237633/46‑1 (MQ=255)                                              gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                    >  1:87146/1‑71 (MQ=255)                                              gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAtt                                             <  1:2344523/46‑1 (MQ=12)                                               atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                              <  1:2751272/60‑1 (MQ=255)                                               atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGac                   >  1:2458871/1‑71 (MQ=255)                                                  tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                          <  1:2823193/45‑1 (MQ=255)                                                    tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                            >  1:1845417/1‑57 (MQ=255)                                                    tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                      >  1:3168975/1‑63 (MQ=255)                                                    tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc               >  1:747842/1‑70 (MQ=255)                                                       ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc            <  1:1257222/70‑1 (MQ=255)                                                       ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc            <  1:2698739/70‑1 (MQ=255)                                                        cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                        <  1:2694289/57‑1 (MQ=255)                                                        cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacc                  <  1:1602284/63‑1 (MQ=255)                                                          ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg        <  1:2205812/71‑1 (MQ=255)                                                          ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg        <  1:757415/71‑1 (MQ=255)                                                          ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg        <  1:662534/71‑1 (MQ=255)                                                           gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                              <  1:178005/48‑1 (MQ=255)                                                           gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                              <  1:3973057/48‑1 (MQ=255)                                                           gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg        >  1:1728911/1‑70 (MQ=255)                                                            ttttCCATCGCGTCGAGCATCATTTCACGGTTCATCACGTTAACGtt                              <  1:3039242/47‑1 (MQ=255)                                                            ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                             <  1:2731694/48‑1 (MQ=255)                                                             tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                       <  1:2926582/37‑1 (MQ=255)                                                             tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                       <  1:2931425/37‑1 (MQ=255)                                                             tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                       <  1:1306678/37‑1 (MQ=255)                                                              ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg    >  1:319676/1‑71 (MQ=255)                                                              ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg    >  1:1585647/1‑71 (MQ=255)                                                              ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat     >  1:3293199/1‑70 (MQ=255)                                                              ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg        <  1:1791464/67‑1 (MQ=255)                                                               tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc   >  1:994847/1‑71 (MQ=255)                                                                ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt  >  1:1545759/1‑71 (MQ=255)                                                                   tCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                        <  1:637657/46‑1 (MQ=255)                                                                    |                                                                   AGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCT  >  minE/608030‑608163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑